MITOCHONDRIAL SUPEROXIDE PRODUCTION AND MNSOD ACTIVITY FOLLOWING EXPOSURE TO AN AGONIST AND ANTAGONISTS OF IONOTROPIC GLUTAMATE RECEPTORS IN RAT BRAIN

¹LIDIJA RADENOVIĆ and ²VESNA SELAKOVIĆ

¹ Department of Physiology and Biochemistry, Faculty of Biology, University of Belgrade^{; 2} Institute for Medical Research, Military Medical Academy, 11000 Belgrade, Serbia and Montenegro

Abstract - The involvement of NMDA and AMPA/kainate receptors in the induction of superoxide production in the rat brain was examined after intrahippocampal injection of kainate, a non-NMDA receptor agonist; kainate plus CNQX, a selective AMPA/kainate receptor antagonist; or kainate plus APV, a selective NMDA receptor antagonist. The measurements took place at different times in the ipsi- and contralateral hippocampus, forebrain cortex, striatum, and cerebellum homogenates. The used glutamate antagonists both ensured sufficient neuroprotection in the sense of lowering superoxide production and raising MnSOD levels, but in the mechanisms and time dynamics of their effects were different. Our findings suggest that NMDA and AMPA/kainate receptors are differentially involved in superoxide production.

This article has been retracted. Retraction: 10.2298/ABS150318026E