TY - JOUR AU - Wang, Dong-sheng AU - Erihemu, Erihemu AU - Yang, Bin PY - 2019/10/23 Y2 - 2024/03/28 TI - Diversity of bacterial communities in the rhizosphere of the endangered plant, Paeonia jishanensis JF - Archives of Biological Sciences JA - Arch Biol Sci VL - 71 IS - 3 SE - Articles DO - UR - https://serbiosoc.org.rs/arch/index.php/abs/article/view/3957 SP - 525-531 AB - <div><p class="author"><strong>Paper description:</strong></p><ul><li><em>Paeonia jishanensis</em> is a significant ancestral species and many cultivated tree peony species were bred from it. <em>P. jishanensis</em> is also a traditional Chinese herbal medicine. The species is facing extinction.</li><li>This study describes for the first time the rhizosphere microbial communities of <em>P. jishanensis</em> with a different growth status.</li><li>Our work suggests that the abundance of many potentially beneficial rhizospheric microbes have decreased with the decline of the plant<em>.</em></li><li>Inoculation of beneficial microbes could be a potential approach to protect those endangered plants.</li></ul><p class="author"><strong>Abstract: </strong>The microbial community in the rhizosphere is thought to provide plants with a second set of genomes, which plays a pivotal role in plant growth. In the present study, soil samples were collected from the rhizosphere of an endangered plant, <em>Paeonia jishanensis</em>. The plants were divided into three groups: well-growing plants, poor-growing plants and dead plants. Metagenomic DNA was isolated from rhizosphere soil samples of these plants and 16S rRNA genes were sequenced by the PacBio-RS II system. The results of taxonomic analysis showed that the dominant phyla were Proteobacteria, Acidobacteria, Planctomycetes, Bacteroidetes and Actinobacteria in all three sample types. Linear discriminate analysis Effect Size (LEfSe) showed that 5 species, <em>Hirschia baltica</em>, <em>Arcobacter aquimarinus</em>, <em>Gimesia maris</em>, <em>Magnetococcus marinus</em> and <em>Pseudoxanthobactor soli</em>, were significantly enriched in the rhizosphere of well-growing plants. Additionally, the results of PCA, MDS and clustering analysis indicated that the bacterial community in the rhizosphere of living <em>P. jishanensis</em> plants was similar. With the death of plants, the bacterial community changed considerably. These findings suggest that the abundance of many beneficial rhizospheric microbes declined with the death of <em>P. jishanensis</em>. This is a potential way to preserve endangered plants by inoculating declining species with beneficial microbes.</p><p><a href="https://doi.org/10.2298/ABS190203036W">https://doi.org/10.2298/ABS190203036W</a></p><p class="affiliation"><strong>Received:</strong> February 3, 2019; <strong>Revised:</strong> May 29, 2019; <strong>Accepted:</strong> May 31, 2019; <strong>Published online: </strong>June 7, 2019</p><p><strong>How to cite this article:</strong> Wang DS, Erihemu, Yang B. Diversity of bacterial communities in the rhizosphere of the endangered plant, <em>Paeonia jishanensis. </em>Arch Biol Sci. 2019;71(3):525-31.</p></div> ER -