ANTIOXIDANT ENZYMES IN THE NEEDLES OF DIFFERENT OMORIKA LINES
JELENA BOGDANOVIĆ1, TANJA DUČIĆ1, N. MILOSAVIĆ2, Z. VUJČIĆ3, MIRJANA ŠIJAČIĆ4, V. ISAJEV4 and KSENIJA RADOTIĆ1
1Center for Multidisciplinary Studies, University of Belgrade,
11000 Belgrade, Serbia and Montenegro2Institute of Chemistry, Technology and Metalurgy, Department of Chemistry
, 11000 Belgrade, Serbia and Montenegro3Faculty of Biochemistry, University of Belgrade
, 11000 Belgrade, Serbia and Montenegro4Faculty of Forestry, University of Belgrade,
11000 Belgrade, Serbia and MontenegroAbstract - Picea omorika (Panè.) Purkynì (Serbian spruce) is a Balkan endemic coniferous species. We studied soluble peroxidase, catalase, polyphenol oxidase, and superoxide-dismutase activity in the needles of five omorika lines grown in a generative seed orchard. The peroxidase and polyphenol oxidase isoenzyme patterns were also investigated. Activity of the studied enzymes varied among different lines. The highest activity of peroxidase, catalase and polyphenol oxidase was found in the A3 ("borealis") and B5 ("semidichotomous") lines. Four acidic and two basic peroxidase isoenzymes and one polyphenol oxidase isoenzyme were detected. There was no variation in either the peroxidase or the polyphenol oxidase isoenzyme pattern among the different lines.