Ultrasound-assisted extraction of peanut shell by-product: chemical properties, antioxidant, and anti-inflammatory effects

Authors

DOI:

https://doi.org/10.2298/ABS240704025K

Keywords:

peanut shell, ultrasound-assisted extraction, luteolin, antioxidant, anti-inflammatory

Abstract

Paper description:

  • Peanut shell extracts have been investigated for their potential bioactive compounds, focusing on antioxidant and anti-inflammatory properties.
  • This study utilized ultrasound-assisted extraction (UAE) to extract these compounds from peanut shells and to evaluate their effects on RAW 264.7 cells. Increased bioactivation and luteolin content via HPLC analysis was confirmed.
  • Ultrasound-assisted peanut shell extract demonstrated enhanced bioactivation, resulting in elevated luteolin levels. The extract exhibited antioxidant and anti-inflammatory effects, as evidenced by increased radical scavenging activities and suppression of NO, PGE2, IL-6, iNOS, and COX-2 expression.
  • Peanut shell extract, obtained through UAE, showed increased antioxidant and anti-inflammatory activities.

Abstract:  Peanut shell by-products have been explored for their pharmacological potential, particularly through applications developed from their utilization. This study aimed to investigate the effects of peanut shell extract (UPE) obtained via ultrasound-assisted extraction (UAE) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. High-performance liquid chromatography analysis revealed elevated levels of luteolin in the ultrasound-extracted peanut shell extract (UPE). UPE demonstrated significant in vitro antioxidant activity, as evidenced by its ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals. The anti-inflammatory effects of UPE were assessed using the nitric oxide (NO) Griess assay, prostaglandin E2 (PGE2), and interleukin-6 (IL-6) enzyme-linked immunosorbent assay (ELISA). Western blot analysis and reverse transcription polymerase chain reaction (RT-PCR) were used to evaluate the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). UPE significantly reduced NO, PGE2, and IL-6 levels in LPS-treated RAW 264.7 cells, suggesting potent anti-inflammatory properties. Furthermore, UPE downregulated the expression of iNOS and COX-2, thereby suppressing NO and PGE2 production. These findings indicate that peanut shell extracts obtained through UAE have therapeutic potential due to their enhanced antioxidant and anti-inflammatory effects, likely attributed to increased levels of luteolin.

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Author Biographies

Jung Wook Kang, College of Fusion and Convergence, Seowon University, 377-3 Musimseoro, Seowon-gu, Cheongju, Chungbuk 28674, Republic of Korea

 

     

In Chul Lee, Department of Cosmetic Science and Technology, Seowon University, 377-3 Musimseoro, Seowon-gu, Cheongju, Chungbuk 28674, Republic of Korea

 

 

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Published

2024-10-25

How to Cite

1.
Kang JW, Lee IC. Ultrasound-assisted extraction of peanut shell by-product: chemical properties, antioxidant, and anti-inflammatory effects. Arch Biol Sci [Internet]. 2024Oct.25 [cited 2024Oct.30];76(3):335-43. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/10023

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Vol. 76 No. 3 (2024)

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Articles

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