In vitro assessment of the nematicidal potential of Streptomyces violascens strain AS2 against root-knot nematodes (Meloidogyne sp.)

Authors

  • Latifa Azlay 1. Laboratory of Microbial Biotechnology, Agrosciences and, Environment, CNRST Labeled Research Unit N◦4, Department of Biology, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco; 2. Laboratory of Biotechnologies and Valorization of Natural Resources, Department of Biology, Faculty of Sciences Agadir, Ibn Zohr University, Agadir, Morocco
  • Ez-Zahra Oubassou 1. Laboratory of Microbial Biotechnology, Agrosciences and, Environment, CNRST Labeled Research Unit N◦4, Department of Biology, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco; 2. Institut de Biologie Moléculaire des Plantes (IBMP), CNRS, Université de Strasbourg, 12, rue du Général Zimmer, 67084 Strasbourg, France
  • Alexandre Berr Institut de Biologie Moléculaire des Plantes (IBMP), CNRS, Université de Strasbourg, 12, rue du Général Zimmer, 67084 Strasbourg, France
  • El Hassan Mayad Laboratory of Biotechnologies and Valorization of Natural Resources, Department of Biology, Faculty of Sciences Agadir, Ibn Zohr University, Agadir, Morocco
  • Mustapha Barakate 1. Laboratory of Microbial Biotechnology, Agrosciences and, Environment, CNRST Labeled Research Unit N◦4, Department of Biology, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco; 2. Biodiversity and Plant Sciences Program, AgroBioScience Department, College of Agriculture and Environmental Sciences, Mohammed VI Polytechnic University (UM6P), 43150 Benguerir, Morocco

DOI:

https://doi.org/10.2298/ABS240711026A

Keywords:

Actinomycetota, Meloidogyne sp., nematocidal activity, egg hatch inhibition, second-stage juvenile mortality

Abstract

Paper description:

  • Chemical nematicides are used in plant parasitic nematode management. Due to their harmful impact on the environment, sustainable solutions are needed.
  • Research focused on Actinobacteria which have diverse mechanisms of action. We studied the in vitro effects of Streptomyces violascens extracts on eggs and second-stage juveniles of Meloidogyne
  • Streptomyces violascens has a significant impact on nematode stages.
  • This work advocates the integration of Streptomyces violascens into sustainable agricultural practices, showing its effectiveness as a green solution on a large scale.

Abstract: Root-knot nematodes (RKNs, Meloidogyne spp.) present a major challenge to sustainable production. The detrimental environmental impacts and increasing resistance associated with synthetic nematicides have increased the need for biocontrol alternatives. Here, we report for the first time the nematicidal properties of Streptomyces violascens and its viability as a safe and effective solution against nematode infestation. In vitro assays showed that aqueous and ethyl acetate extracts of S. violascens significantly impeded Meloidogyne sp's reproductive and locomotive functions. The aqueous extract displayed remarkable efficacy, completely inhibiting egg hatching at a concentration of 600 µg/mL, with an LC50 of 168.38 µg/mL, and causing 99% immobility of second-stage juveniles (J2s) at 800 µg/mL within 72 h. Conversely, the organic extract exhibited a 29.3% hatching rate (LC50 of 849.26 µg/mL) and 79% immobility of J2s under comparable conditions. These findings point to the robust nematicidal potential of S. violascens extracts. Enzymatic profiles supported these results, with the selected isolate producing chitinase and protease enzymes capable of degrading nematode eggshells. Our results show that the S. violascens strain can be integrated into a sustainable nematode management practice as an important agent of RKN biocontrol.

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2024-10-25

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1.
Azlay L, Oubassou E-Z, Berr A, Mayad EH, Barakate M. In vitro assessment of the nematicidal potential of Streptomyces violascens strain AS2 against root-knot nematodes (Meloidogyne sp.). Arch Biol Sci [Internet]. 2024Oct.25 [cited 2024Dec.22];76(3):345-58. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/10041

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Vol. 76 No. 3 (2024)

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Copyright (c) 2024 latifa azlay, ez-zahra oubassou, alexandre berr, el hassan mayad, mustapha barakate

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