miR-146a expression and the miR-146a gene variant rs2910164 as diagnostic and prognostic biomarkers of oral cancer
DOI:
https://doi.org/10.2298/ABS240711032VKeywords:
oral cancer, miR-146a-5p, rs2910164, biomarkerAbstract
Paper description:
- There is a growing interest in identifying new molecular biomarkers among miRNAs for diagnosis and prognosis of oral cancer.
- The study involved 35 oral cancer patients. The expression of miR-146a-5p in cancerous and adjacent non-cancerous tissue and the genotype of the rs2910164 variant were determined using real-time PCR.
- Oral cancer patients with high and low miR-146a-5p expression differed significantly in overall survival. Patients with the GG genotype and high miR-146a-5p expression had poor survival.
- A high miR-146a-5p level is a potential biomarker for poor survival in oral cancer patients.
Abstract: This study aimed to analyze the diagnostic and prognostic significance of miR-146a-5p in oral cancer and the association of the rs2910164 gene variant with miR-146a-5p expression. Thirty-five oral cancer patients participated in the study. Expression of miR-146a-5p in oral cancer, adjacent non-cancerous tissue, and the rs2910164 genotype variant were examined by real-time PCR. No difference was observed in miR-146a-5p expression between cancerous and non-cancerous tissues (P=0.272). It was concluded that miR-146a-5p is not a good diagnostic biomarker. There was no difference in miR-146a-5p expression depending on the rs2910164 genotype. The rs2910164 variant was associated with tumor histological grade (P=0.037), stage (P=0.036), and lymph node status (P=0.025). There was a difference in survival between oral cancer patients with high and low miR-146a-5p expression (P=0.026), but not between patients with the GG and GC genotypes (P=0.400). Oral cancer patients with the wild-type GG genotype and high miR-146a-5p expression had significantly shorter survival when compared to patients with miR-146a-5p expression below the optimal cut-off and the wild-type genotype (P=0.035). A high miR-146a-5p level could be considered a potential biomarker of poor survival in patients with oral cancer, especially in those with the wild-type GG genotype of the rs2910164 variant.
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