Response of Virginia (flue-cured) tobacco genotypes to water-deficit stress


  • Lydia Shtereva Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences
  • Elisaveta Stoimenova Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences
  • Marina Drumeva-Yoncheva Tobacco and Tobacco Products Institute, Plovdiv
  • Bistra Michailova Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences
  • Tania Kartzeva Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences
  • Roumiana Vassilevska-Ivanova Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences


tobacco plant, drought stress, proline, hydrogen peroxide, lipid peroxidation


The effect of prolonged water deficit on four Virginia (flue-cured) tobacco genotypes, Line 842, Oxford 207, RG11 and Virgin D, was analyzed in whole plants. Drought stress was induced by withholding irrigation and subjecting plants to low, moderate and severe regimes. Some growth indices such as fresh weight, plant growth rate, number, color and area of new developed leaves, as well as proline, hydrogen peroxide (H2O2) and malondialdehyde (MDA) content as a measure of oxidative stress were investigated to examine the role of genotype in water-deficit tolerance. Under stress, the weight of the aboveground parts of plants, plant growth height, number of new developed leaves and leaf area index decreased with the severity of treatment. The stressed plants accumulated more proline, malonildialdehide and hydrogen peroxide than control non-stressed plants under water-deficit conditions. The results showed that among the genotypes, Virgin D (VD) was the most sensitive to drought, while L 842 and Oxford 207 were moderately tolerant; RG11 was drought-tolerant. This suggests that the correlation between the physiological traits and level of antioxidative response exists and therefore it could be used as a rapid screening test to evaluate the drought tolerance of tobacco.

DOI: 10.2298/ABS160202085S

Received: February 2, 2016; Revised: May 27, 2016; Accepted: June 24, 2016; Published online: September 23, 2016

How to cite this article: Shtereva L, Stoimenova E, Drumeva-Yoncheva M, Michailova B, Kartzeva T, Vassilevska-Ivanova R. Response of Virginia (flue-cured) tobacco genotypes to water-deficit stress. Arch Biol Sci. 2017;69(1):119-27.


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Author Biographies

Lydia Shtereva, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Applied Genetics and Biotechnology

Assoc. Prof. Dr.

Elisaveta Stoimenova, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Applied Genetics and Biotechnology


Marina Drumeva-Yoncheva, Tobacco and Tobacco Products Institute, Plovdiv

Tobacco Breeding

Research Fellow

Bistra Michailova, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Applied Genetics and Biotechnology

Research Fellow

Tania Kartzeva, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Applied Genetics and Biotechnology


Roumiana Vassilevska-Ivanova, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences

Applied Genetics and Biotechnology

Assoc. Prof.


Ashraf M. Inducing drought tolerance in plants: Recent advances. Biotechnol Adv. 2010; 28:169-83.

Tuteja N, Gill SS, Tuteja R. Improving crop productivity in sustainable agriculture. Wiley-Blackwell; 2012. 536 p.

Bray EA, Bailey-Serre J, Weretylnik E. Responses to abiotic stresses. In: Buchanan B, Gruissem W, Jones R, editors. Biochemistry and molecular biology of plants. Rockville: American society of plant physiologists; 2000. p. 1158-203.

Chaves MM, Maroco JP, Pereira JS. Understanding plant responses to drought - from genes to the whole plant. Funct Plant Biol. 2003;30(3):239-64.

Wang W, Vinocur B, Altman A. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta. 2003;218:1-14.

Farooq M. Wahid A, Kobayashi N, Fujita D, Basra SMA. Plant drought stress: effects mechanisms and management. Agron Sustainable Dev.2009;29:185-212.

Alscher RG, Donahue JL, Cramer CL. Reactive oxygen species and antioxidants: relationships in green cells. Physiol Plant 1997;100:224-33.

Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 2002;7:405-10.

Neill S, Desikan R, Hancock J. Hydrogen peroxide signaling. Curr Opin Plant Biol. 2002;5:388-95.

Imlay JA. Pathways of oxidative damage. Annu Rev Microbiol. 2003;57:395-418.

Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R. Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant Cell Environ. 2010;33:453-67.

Sakamoto A, Murata N. The role of glycine betaine in the protection of plants from stress: clues from transgenic plants. Plant Cell Environ. 2002;25:163-71.

Luna C, Garcia-Seffino L, Arias C, Taleisnik E. Oxidative stress indicators as selection tools for salt tolerance. Plant Breed. 2000;119(4):341-45.

Hernandez JA, Almansa MS. Short-term effects of salt stress on antioxidant systems and leaf water relations of pea leaves. Physiol Plant. 2002;115:251-57.

Meloni DA, Oliva MA, Martinez CA, Cambraia J. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Env Exp Bot. 2003; 49(1):69-76.

Filippou P, Bouchagier P, Skotti E, Fotopoulos V. Proline and reactive oxygen/nitrogen species metabolism is involved in the tolerant response of the invasive plant species Ailanthus altissima to drought and salinity. Env Exp Bot. 2014;97 (1):1-10

Sairam RK, Srivastava GC. Water stress tolerance of wheat (Triticum aestivum L.): variations in hydrogen peroxide accumulation and antioxidant activity in tolerant and susceptible genotypes. J Agron Crop Sci. 2001;186(1):63-70.

Sparrow GN, Miles JD, Stansell R. Irrigation of flue-cured tobacco. Transactions of the ASAE. 1966;9(1):132-34.

McNee P, Warrell LA, van den Muyzenberg EWB. Influence of water stress on yield and quality of flue-cured tobacco. Aust J Exp Agr Anim Husbandry. 1978;18:726-31.

Çakir R, Cebi U. Growth and dry matter accumulation dynamics of flue-cured tobacco under different soil moisture regimes. J Agron. 2006;5:79-86.

Maw BW, Stansell JR, Mullinix BG. Soil-plant-water relationships for flue-cured tobacco. University of Georgia. Res Bull Georgia Agricul Exp Station. 2009;427:1-40.

Biglouei MH, Assimi MH, Akbarzadeh A. Effect of water stress at different growth stages on quantity and quality traits of Virginia (flue-cured) tobacco type. Plant Soil Environ. 2010;56(2):67-75.

Wilkinson CA, Reed TD, Johns JL. Flue-cured tobacco variety information for 2002. Virginia: Polytechnic Institute and State University Press; 2002. 447 p.

Çakir R, Cebi U. Yield, water use and yield response factor of flue-cured tobacco under different levels of water supply at various growth stages. Irrig Drain. 2010;59:453-64.

Yuan YB, Chen DY, Shao XH, Li YH, Ding F, Kwizera C. Effects of different irrigation quantities on plant growth and photosynthesis characters of flue-cured tobacco. J Food Agric Environ. 2012;10(2):1160-63.

Chinchev B. Study of introduced and new varieties of tobacco Virginia. [dissertation].[Plovdiv]: Faculty of Agriculture, University of Plovdiv;1989. 297 p

Riga P, Vartanian N. Sequential expression of adaptive mechanisms is responsible for drought resistance in tobacco. Aust J Plant Physiol. 1999;26:211-20.

Tsai YJ, Maw BW. Effects of water deficit on tobacco growth: a modeling approach. ASAE. St. Joseph, Mich. 1988;19.

Celik O, Atak C. The effect of salt stress on antioxidative enzymes and proline content of two Turkish tobacco varieties. Turk J Biol. 2012;36:339-56.

Yonchev Y. Reaction of introduced tobacco varieties type Virginia to PVY and TMV − economically important virus diseases. Tobacco (Prilep, Macedonia). 2008;58(1-2):41-5.

Yonchev Y, Stoimenova E. Monitoring of viruses causing economically important diseases in tobacco. Plant Protect (Macedonia). 2009;20:111-6.

Hunt R. Growth analysis, individual plants. In: Thomas B, Murphy DJ, Murray D, editors. Encyclopaedia of applied plant sciences. London: Academic Press; 2003. p. 579-88.

Moustakas NK, Ntzanis H. Estimating flue-cured tobacco leaf area from linear measurements under Mediterranean conditions. Agric Med. 1998;128:226-31.

Ritchie SW., Nguyen HT, Holaday AS. Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Sci. 1990;30:105-11.

Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant Soil. 1973;39:205-7.

Alexieva V, Sergiev I, Mapelli S, Karanov E. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environ. 2001;24(12):1337-44.

Cakmak I, Horst WJ. Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiol Plant.1991;83:463-8.

Quan LJ, Zhang B, Shi WW, Li HY. Hydrogen peroxide in plants: a versatile molecule of the reactive oxygen species network. J Integrat Plant Biol. 2008;50:2-18.

Aspinall D, Paleg LG. Proline accumulation: physiological aspects. In: L. Paleg, D. Aspinall editors. The Physiology and Biochemistry of Drought Resistance in Plants. Sidney: Academic Press: 1981. p. 215-28.

Jaleel CA, Gopi R, Manivannan P, Gomathinayagam M, Riadh K, Ines J, Chang-Xing Z, Hong-Bo S, Panneerselvam R. Antioxidant defense responses: Physiological plasticity in higher plants under abiotic constraints. Acta Physiol Plant. 2009;31(3):427-36.

Liang X, Zhang L, Natarajan SK, Becker DF. Proline mechanisms of stress survival. Antioxidants Redox Signaling. 2013;19(9):998-1011.

Heuer B. Osmoregulatory role of proline in water and salt-stressed plants. In: Pessarakli, M. editor. Handbook of Plant and Crop Stress. New York: 1994. p. 363-81.

Sankar B, Jaleel CA, Manivannan P, Kishorekumar A, Somasundaram R, Panneerselvam R. Drought-induced biochemical modifications and proline metabolism in Abelmoschus esculentus (L.) Moench. Acta Bot Croat. 2007;61:43-56.

Szabados L, Savoure A. Proline: a multifunctional amino acid. Review. Trends Plant Sci. 2009;15(2):89 - 97.

Zaifnejad M, Clark RB, Sullivan CY. Aluminum and water stress effects on growth and proline of Sorghum. J Plant Physiol. 1997;150:338-44.

Hanson AD, Nelsen CE, Evanson EH. Evaluation of free proline accumulation as an index of drought resistance using two contrasting barley cultivars. Crop Sci. 1977;17:720-6.

Al-Karaki GN, Clark RB, Sullivan CY. Phosphorus nutrition and water stress effects on proline in sorghum and bean. J Plant Physiol. 1996;148:745-51.

Türkan I, Bor M, Ozdemir F, Koca H. Differential response of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci. 2005;168:223-31.

Dobra J, Vankova R, Havlova M, Burman AJ, Libus Т, Storchova H. Tobacco leaves and roots differ in the expression of proline metabolism-related genes in the course of drought stress and subsequent recovery. J Plant Physiol. 2011;168:1588-97.

Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4- hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med. 1991;11:81-128.

Tatar O, Gevrek MN. Influence of water stress on proline accumulation, lipid peroxidation and water content of wheat. Asian J Plant Sci. 2008;7(4):409-12.

Pandey HC, Baig MJ, Chandra A, Bhatt RK. Drought stress induced changes in lipid peroxidation and antioxidant system in genus Avena. Environ Biol. 2010;31:435-40.

Wu GQ, Zhang LN, Wang YY. Response of growth and antioxidant enzymes to osmotic stress in two different wheat (Triticum aestivum L.) cultivars seedlings. Plant Soil Environ. 2012;58(12):534-9.

Baloğlu MC, Kavas M, Aydın G, Öktem HA, Yücel AM. Antioxidative and physiological responses of two sunflower (Helianthus annuus) cultivars under PEG-mediated drought stress. Turk J Bot. 2012;36:707-14.

Khanna-Chopra R, Selote D. Acclimation to drought stress generates oxidative stress tolerance in drought-resistant than -susceptible wheat cultivar under field conditions. Environ Exp Bot. 2007;60(2):276-83.

Prasad TK, Anderson MD, Martin BA, Stewart CR. Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide. Plant Cell. 1994;6:65-74.

Foyer C, Lorez-Delgao H, Dat J, Scott I. Hydrogen peroxide- and glutathione-associated mechanisms of acclimatory stress tolerance and signaling. Physiol Plant. 1997;100:241-54.

Foyer CH, Noctor G. Oxidant and antioxidant signaling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell Environ. 2005;29:1056-57.




How to Cite

Shtereva L, Stoimenova E, Drumeva-Yoncheva M, Michailova B, Kartzeva T, Vassilevska-Ivanova R. Response of Virginia (flue-cured) tobacco genotypes to water-deficit stress. Arch Biol Sci [Internet]. 2017Mar.7 [cited 2024Apr.22];69(1):119-27. Available from: