IMPACT OF APPLICATION OF ZINC OXIDE NANOPARTICLES ON CALLUS INDUCTION, PLANT REGENERATION, ELEMENT CONTENT AND ANTIOXIDANT ENZYME ACTIVITY IN TOMATO (SOLANUM LYCOPERSICUM MILL.) UNDER SALT STRESS
Abstract
The properties of nanomaterials and their potential applications have been given considerable attention by researchers in various fields, especially agricultural biotechnology. However, not much has been done to evaluate the role or effect of zinc oxide nanoparticles (ZnO-NPs) in regulating physiological and biochemical processes in response to salt-induced stress. For this purpose, some callus growth traits, plant regeneration rate, mineral element (sodium, potassium, phosphorous and nitrogen) contents and changes in the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in tissues of five tomato cultivars were investigated in a callus culture exposed to elevated concentrations of salt (3.0 and 6.0 g L-1NaCl), and in the presence of zinc oxide nanoparticles (15 and 30 mg L-1). The relative callus growth rate was inhibited by 3.0 g L-1 NaCl; this was increased dramatically at 6.0 g L-1. Increasing exposure to NaCl was associated with a significantly higher sodium content and SOD and GPX activities. Zinc oxide nanoparticles mitigated the effects of NaCl, and in this application of lower concentrations (15 mg L-1) was more effective than a higher concentration (30 mg L-1). This finding indicates that zinc oxide nanoparticles should be investigated further as a potential anti-stress agent in crop production. Different tomato cultivars showed different degrees of tolerance to salinity in the presence of ZnO-NPs. The cultivars Edkawy, followed by Sandpoint, were less affected by salt stress than the cultivar Anna Aasa.
Key words: Nano biotechnology, in vitro culture, protein, salinity, superoxide dismutase, glutathione peroxidase
Received: November 5, 2015; Revised: February 11, 2016; Accepted: February 12, 2016; Published online: March 21, 2016
How to cite this article: Alharby HF, Metwali EMR, Fuller MP, Aldhebiani AY. Impact of application of zinc oxide nanoparticles on callus induction, plant regeneration, element content and antioxidant enzyme activity in tomato (Solanum lycopersicum Mill.) under salt stress. Arch Biol Sci. 2016;68(4):723-35.
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References
FAOSTAT database. Production Crops [Internet]. Food and Agriculture Organization of the United Nations. 1998- [cited 2015 Jun 22]. Available from: http://faostat3.fao.org/browse/Q/QC/E.
Manaa A, Faurobert M, Valot B, Bouchet J, Grasselly D, Cause M, Ahmed H. Effect of salinity and calcium on tomato fruit proteome. Omics. 2013;17(6):338-52.
Tester M, Langridge P. Breeding technologies to increase crop production in a changing world. Sci. 2010; 327(5967):818-822.
Wieczorek A. 2003. Use of biotechnology in agriculture – benefits and risks. Honolulu (HI): University of Hawaii. 6 p. (Biotechnology; BIO-3).
Debergh PC, Read PE. Micropropagation. In: Debergh PC, Zimmerman RH, editors. Micropropagation. Dordrecht, Boston, London: Kluwer Academic Publishers; 1991. p. 1-13.
Shibli R, Kushad M, Yousef G, Lila MA. Physiological and biochemical responses of tomato microshoots to induce salinity stress with associated ethylene accumulation. Plant Growth Regul. 2007;51(2):159-69.
Chebet DK, Okeno JA, Mathenge P. Biotechnological approaches to improve horticultural crop production. Acta Hortic. 2003;625: 473-7.
Shatnawi M, Johnson K, Torpy F. In vitro propagation and cryostorage of Syzygium francisii (Myrtaceae) by encapsulation-dehydration method. In vitro Cell Dev Biol-Plant. 2004;40 (4):403-7.
Nair R, Varghese SH, Nair BG, Maekawa T, Yoshida Y, Kumar DS. Nanoparticulate material delivery to plants. Plant Sci. 2010;179(3):154-63.
Reynolds GH. Forward to the future nanotechnology and regulatory policy. Pacific Res Inst. 2002;24:1-23.
Sheikh N, Hassanzadeh G, Baghestani M, Zabd B. Study the effect of zinc foliar application on the quantitative yield of grain maize under water stress. Electro J Crop Prod. 2009;2(2):59-74.
Prasad T, Sudhakar P, Sreenivasulu Y, Latha P, Munaswamy V, Raja Reddy K, Sreeprasad TS, Sajanlal PR, Pradeep T. Effect of nanoscale zinc oxide particles on the germination, growth and yield of peanut. J Plant Nutr. 2012;35(6):905-27.
Munns R, Tester M. Mechanisms of salinity tolerance. Annu Rev Plant Biol. 2008;59:651-81.
Hasanuzzaman M, Hossain MA, da Silva JAT, Fujita M. Plant responses and tolerance to abiotic oxidative stress: antioxidant de- fense is a key factor. In: Venkateshwarulu B, Shanker AK, Shanker C, Mandapaka M, editors. Crop stress and its management: perspectives and strategies. Springer, Berlin. 2012. p. 261–315.
Gill SS, AnjumNA, Gill R, Yadav S, Hasanuzzaman M, Fujita M, Mishra P, Sabat SC, Tuteja N. Superoxide dismutase-mentor of abiotic stress tolerance in crop plants. Environ Sci Pollut Res. 2015;22(14):10375-94.
Gautier H, Lopez-Lauri F, Massot C, Murshed R, Marty I, Grasselly D, Keller C, Sallanon H, Genard M. Impact of ripening and salinity on tomato fruit ascorbate content and enzymatic activities related to ascorbate recycling. Funct Plant Sci Biotechnol. 2010;4:66-75.
Sekmena AH, Türkan I, Takio S. Differential responses of antioxidative enzymes and lipid peroxidation to salt stress in salt-tolerant Plantago maritima and salt-sensitive Plantago media. Physiol Plant. 2007;131(3):399-411.
Lopez F, Vansuyt G, Casse-Delbart F, Fourcroy P. Ascorbate peroxidase activity, not the mRNA level, is enhanced in salt stressed Raphanus sativus plants. Physiol Plant. 1996;97(1):13-20.
Jbir N, Chaibi W, Ammar S, Jemmali A, Ayadi A. Root growth and lignification of two wheat species differing in their sensitivity to NaCl, in response to salt stress. Comptes Rendus Acad Sci III. 2011;324:863-8.
Helaly MN, El-Metwally MA, El-Hoseiny H, Omar SA, El-Sheery NI. Effect of nanoparticles on biological contamination of in vitro cultures and organogenic regeneration of banana. Aust J Crop Sci. 2014;8(4):612-24.
Metwali EMR. Improving abiotic stress resistance in cauliflower (Brassica oleracea var. botrytis L.) by mutagenesis and Agrobacterium mediated transformation. [PhD thesis]. [Plymouth]: School of Biological Science, University of Plymouth, 2006. 110 p.
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15(3):4734-97.
Metwali M R E, Hemaid I E Soliman, Fuller M P, Omar A A. Improving fruit quality in tomato (Lycopersicum esculantum L.) under heat stress by silencing the Vir1 gene using small interfering RNA technology. Plant Cell Tiss Org Cult. 2015;121(1):153-66.
El-Sayed A, Mahdia G, Fouad H, Mona Ismail. Responses to NaCl Salinity of Tomato Cultivated and Breeding Lines Differing in Salt Tolerance in Callus Cultures. Int J Agric Biol. 2004;6(1):19-26.
Soliman I E S, Metwali M R E, Omar A A. Micropropagation of Stevia rebaudiana Betroni and assessment of genetic stability of in vitro regenerated plants using inter simple sequence repeat (ISSR) marker. Plant Biotechnol NAR. 2014;31(3):249-56
Chen JY, Yue RQ, Xu HX, Chen XJ. Study on plant regeneration of wheat mature embryos under endosperm-supported culture. Agric Sci China. 2006;5(8):572-8.
Soliman I A, Mohamed H. Selection for Drought Tolerance Genotypes in Durum Wheat (Triticum durum Desf.) Under In vitro Conditions. Middle-East J Sci Res. 2013;14:69-78.
El-Wanis A, Mona M, Abdel-Baky M, Salman S. Effect of grafting and salt stress on the growth, yield and quality of cucumber grown in NFT system. J Appl Sci Res. 2012;8(10):5059-67.
Jackson M. Soil chemical analysis. Englewood Cliffs, New Jersey: Prentic-Hall Inc.; 1958. p. 498.
Richards LA. Diagnosis and improvement of saline and alkali soils. Handbook United States Department of Agriculture, Washington DC; 1954. 160 p.
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1-2):248-54.
Beyer WF, Fridovich I. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem. 1987;161(2):559-66.
Dhindsa RS, Plumb-Dhindsa P, Thorpe TA. Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase. J Exp Bot. 1981;32(1):93-101.
Roxas VP, Smith RK, Allen ER, Allen RD. Overexpression of glutathione S-transferase/ glutathione peroxidase enhances the growth of transgenic tobacco seedlings during stress. Nat Biotechnol. 1997;15(10):988-91.
Ghormade V, Deshpande MV, Paknikar KM. Perspectives for nano-biotechnology enabled protection and nutrition of plants. Biotechnol Adv. 2011;29(6):792-803.
Nair R, Varghese SH, Nair BG, Maekawa T, Yoshida Y, Kumar DS. Nanoparticulate material delivery to plants. Plant Sci. 2010;179(3):154-63.
Chen H, Yada R. Nanotechnologies in agriculture: New tools for sustainable development. Trends Food Sci Tech. 2011;22(11):585-94.
Ahmad MSA, Javed F, Ashraf M. Iso-osmotic effect of NaCl and PEG on growth, cations and free proline accumulation in callus tissue of two indica rice (Oryza sativa L.) genotypes. Plant Growth Regul. 2007;53(1):53-63.
Mahdi1 E, Idris T. The effects of NaCl pre-treatment on salt tolerance of tomato (Lycopersicon esculentum Mill.) callus grown under elevated saline conditions. Int Res J Biotechnol. 2013;4(3):61-7.
Babu MA, Singh D, Gothandam KM The effect of salinity on growth, hormones and mineral elements in leaf and fruit of tomato cultivar PKM1. J Anim Plant Sci 2012;22(1):159-64.
May MJ, Vernoux T, Leaver C, Van Montagu M, Inze D. Glutathione homeostasis in plants: implications for environmental sensing and plant development. J Exp Bot. 1998;49(321):649-67.
Alpaslan M, Inal A, Gunes A, Cıkılı Y, Ozcan H. Effect of zinc treatment on the alleviation of sodium and chloride injury in tomato (Lycopersicum esculentum L.) Mill. cv. Lale) grown under salinity. Turk J Agric For. 1999;23(1):1-6.
Alloway BJ. Zinc in soils and crop nutrition. Brussels: International zinc association; 2004. p 28.
Weisany W, Sohrabi Y, Heidari G, Siosemardeh A, Ghassemi-Golezani K. Changes in antioxidant enzymes activity and plant performance by salinity stress and zinc application in soybean (Glycine max L.). Plant Omics J. 2012;5(2):60-7.
Keisling T C, Lauer D A, Walker M E, Henning R 1. Visual, tissue, and soil factors associated with Zn toxicity of peanuts. Agron J. 1977;69(5):765-9.
Davis-Carter JG, Parker MB, Gaines TP. Interaction of soil zinc, calcium and pH with zinc toxicity in peanuts. In: Wright R, Baligar C, Murrmann R, editors. Plant-soil interactions at low pH. Dordecht, Boston, London: Kluwer Academic Publisher; 1991. p. 339-47.
Camp AF. Zinc as a nutrient in plant growth. Soil Sci. 1945;60(2):57-164.
Gall O E, Barnette R M. Toxic limits of replaceable zinc to corn and cowpeas grown on three Florida soils. J Am Soc Agron. 1940;32(1):23-32.
Sokolov PS, Baranov AN, Dobrokhotova ZV, Solozhenko VL. Synthesis and thermal stability of rock-salt ZnO in salt nanocomposites. Russ Chem Bull. 2010;59(2):325-8.
Tari I, Csiszár J, Horváth E, Poór P, Takács Z, Szepesi A. The Alleviation of the Adverse Effects of Salt Stress in the Tomato Plant by Salicylic Acid Shows A Time-and Organ-Specific Antioxidant Response. Acta Biol Crac Ser Bot. 2015;57(1):1-10.
Lokhande VH, Nikam TD, Suprasanna P Biochemical, physiological and growth changes in response to salinity in callus cultures of Sesuvium portulacastrum L. Plant Cell Tiss Organ Cult. 2010;102(1):17-25.
Murshed R, Lopez-Lauri F, Sallanon H. Effect of water stress on antioxidant systems and oxidative parameters in fruits of tomato (Solanum Lycopersicon L, cv. Micro-tom). Physiol Mol Biol Plants. 2014;20(1):15-29.
Avsian-Kretchmer O, Eshdat Y, Gueta-Dahan Y, Ben- Hayyim G. Regulation of stress induced phospholipid hydroperoxide glutathione peroxidase expression in citrus. Planta, 1999;209(4):469-77.
Srinieng K, Saisavoey T, Karnchanatat A. Effect of salinity stress on antioxidative enzyme activities in tomato cultured in vitro. Pak J Bot. 2015;47(1):1-10.
Tavallali V, Rahemi M, Eshghi S, Kholdebarin B, Ramezanian A. Zinc alleviates salt stress and increases antioxidant enzyme activity in the leaves of pistachio seedlings. Turkey J Agric For. 2010;34(4):349-59.
Parida AK, Das AB. Salt tolerance and salinity effects on plants: a review. Ecotoxicol Environ Safe. 2005;60(3):324-49.
Tavallali V, Rahemi M, Maftoun M, Panahi B, Karimi S, Ramezanian A, Vaezpour M. Zinc influence and salt stress on photosynthesis, water relations, and carbonic anhydrase activity in pistachio. Sci Hortic. 2009;123(2):272-9.
Cakmak I. Possible roles of zinc in protecting plant cells from damage by reactive oxygen species. New Phytol. 2000;146(2):185-205.
Raskar SV, Laware SL. Effect of zinc oxide nanoparticles on cytology and seed germination in onion. Int J Curr Microbiol App Sci. 2014;3(2):467-73.
Mazaherinia S, Astaraei AR, Fotovat A, Monshi A. Nano iron oxide particles efficiency on Fe, Mn, Zn and Cu concentrations in wheat plant. Word App Sci J. 2010;7(1):36-40.
Abdul-Qados AMS. Mechanism of nanosilicon-mediated alleviation of salinity stress in faba bean (Vicia faba L.) Plants. Am J Exp Agric. 2015;7(2):78-95.
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