DNA methylation alteration is a major consequence of genome doubling in autotetraploid Brassica rapa

Authors

  • Yanhao Xu 1. Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland and Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, Hubei 434025; 2. Hubei Collaborative Innovation Center for Grain Industry, College of Agriculture, Yangtze University, Jingzhou, Hubei 434002
  • Wenying Zhang 1. Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland and Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, Hubei 434025; 2. Hubei Collaborative Innovation Center for Grain Industry, College of Agriculture, Yangtze University, Jingzhou, Hubei 434002
  • Gonghai Chen Jingzhou Academy of Agricultural Sciences, Jingzhou, 434100, Hubei
  • Jianbo Wang State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072

Keywords:

autotetraploid, Brassica rapa, DNA methylation, gene expression, genome doubling

Abstract

Polyploids are typically classified as autopolyploids or allopolyploids based on the origin of their chromosome sets. Autopolyploidy is much more common than traditionally believed. Allopolyploidization, accompanied by genomic and transcriptomic changes, has been well investigated. In this study, genetic, DNA methylation and gene expression changes in autotetraploid Brassica rapa were investigated. No genetic alteration was detected using an amplified fragment length polymorphism (AFLP) approach. Using a cDNA-AFLP approach, approximately 0.58% of fragments showed changes in gene expression in autotetraploid B. rapa. The methylation-sensitive amplification polymorphism (MSAP) analysis showed that approximately 1.7% of the fragments underwent DNA methylation changes upon genome doubling, with hypermethylation and demethylation changes equally affected. Fragments displaying changes in gene expression and methylation status were isolated and then sequenced and characterized, respectively. This study showed that variation in cytosine methylation is a major consequence of genome doubling in autotetraploid Brassica rapa.

https://doi.org/10.2298/ABS170131015X

Received: January 31, 2017; Revised: April 6, 2017; Accepted: May 4, 2017; Published online: May 23, 2017

How to cite this article: Xu Y, Zhang W, Chen G, Wang J. DNA methylation alteration is a major consequence of genome doubling in autotetraploid Brassica rapa. Arch Biol Sci. 2017;69(4):689-97.

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Published

2017-10-18

How to Cite

1.
Xu Y, Zhang W, Chen G, Wang J. DNA methylation alteration is a major consequence of genome doubling in autotetraploid Brassica rapa. Arch Biol Sci [Internet]. 2017Oct.18 [cited 2024Dec.22];69(4):689-97. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/1431

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Vol. 69 No. 4 (2017)

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