In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra

Authors

  • Vanja Vuksanović University of Novi Sad, Faculty of agriculture, 21000 Novi Sad
  • Branislav Kovačević University of Novi Sad, Institute of Lowland Forestry and Environment, 21000 Novi Sad
  • Marina Katanić University of Novi Sad, Institute of Lowland Forestry and Environment, 21000 Novi Sad
  • Saša Orlović University of Novi Sad, Institute of Lowland Forestry and Environment, 21000 Novi Sad
  • Dragana Miladinović Institute of Field and Vegetable Crops, Novi Sad

Keywords:

European black poplar, phytoextraction, low pH, tissue culture, microwave sterilization,

Abstract

Phytoextraction is an efficient and cheap way to extract copper from soils in riparian zones. In this work five genotypes of the endangered tree species Populus nigra L. were tested for their copper tolerance and accumulation in vitro when cultivated on media with three Cu concentrations: 10-3, 10-4 and 10-7 M (buffered with citric acid/Na-citrate buffer, pH 3 before sterilization). After five-weeks cultivation of rooted shoots, the highest increases in morphological and biomass parameters were observed at 10-7 M Cu2+. As the medium with 10-3 M Cu2+ exhibited a toxic effect, the effect of 10-4 M Cu2+ and pH 3 was used for further genotype evaluation. According to the measured morphological and parameters of photosynthetic pigment contents, the best performance was achieved by the genotype Populus nigra cl. DN3. The highest copper accumulation on the same medium was achieved by genotype Populus nigra cl. BN5. The obtained data point to the considerable potential of the applied method in the evaluation of Populus nigra genotypes for use in projects of copper phytoextraction.

https://doi.org/10.2298/ABS170210014V

Received: February 10, 2017; Revised: April 6, 2017; Accepted: April 26, 2017; Published online: May 15, 2017

How to cite this article: Vuksanović V, Kovačević B, Katanić M, Orlović S, Miladinović D. In vitro evaluation of copper tolerance and accumulation in Populus nigra. Arch Biol Sci. 2017;69(4):679-87.

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References

Arora M, Kiran B, Rani S, Rani A, Kaur B, Mittal N. Heavy metal accumulation in vegetables irrigated with water from different sources. Food Chem. 2008;111:811-15.

Van Assche F, Clijsters H. Effects of metals on enzyme activity in plants. Plant Cell Environ. 1990;13:195-206.

Burzyński M, Buczek J. Uptake and assimilation of ammonium ions by cucumber seedlings from solutions with different pH and addition of heavy metals. Acta Soc Bot Pol. 1998;76:197-200.

Oleksyn J, Karolewski P, Giertych MJ, Werner A, Tjoelker MG, Reich PB. Altered root growth and plant chemistry of Pinus sylvestris seedlings subjected to aluminum in nutrient solution. Trees. 1996;10:135-44.

Landis TD, Van Steenis E. Micronutrients: Copper. Tree Plant Notes. 2000;49(3):44-8.

Sakan SM, Đorđević DS, Manojlović DD, Polić PS. Assessment of heavy metal pollutants accumulation in the Tisza river sediments. J Environ Manage. 2009;90:3382-90.

Antonijević M, Marić M. Determination of the content of heavy metals in pyrite contaminated soil and plants. Sensors. 2008;8:5857-65.

Bozkurt MA, Yarilga T. The effects of sewage sludge applications on the yield, growth, nutrition and heavy metal accumulation in apple trees growing in dry conditions. Turk J Agric For. 2003;27:285-92.

Pivetz BE. Ground water issue: Phytoremediation of contaminated soil and ground water at hazardous waste sites. Washington, DC: U.S. Environmental Protection Agency, Technology Innovation Office, Office of Solid Waste and Emergency Response; 2001. 36 p.

Barcelo J, Poschenrieder C. Phytoremediation: principles and perspectives. Contrib Sci. 2003;2:333-44.

Ghosh M, Singh SP. A review on phytoremediation of heavy metals and utilization of its byproducts. Appl Ecol Env Res. 2005;3:1-18.

Pulford ID, Watson C. Phytoremediation of heavy metal-contaminated land by trees - a review. Environ Int. 2003;29:529-40.

Di Lonardo S, Capuana M, Arnetoli M, Gabbrielli R, Gonnelli C. Exploring the metal phytoremediation potential of three Populus alba L. clones using an in vitro screening. Environ Sci Pollut R. 2011; 18: 82-90.

Borghi M, Tognetti R, Monteforti R, Sebastiani L. Responses of Populus×euramericana (P. deltoides×P. nigra) clone Adda to increasing copper concentrations. Environ Exp Bot. 2007;61:66-73.

Aitchison EW, Kelley SL, Alvarez PJJ, Schoor JL. Phytoremediation of 1,4-dioxane by hybrid poplar trees. Water Environ Res. 2000;72:313-21.

Kovačević B, Tomović Z, Štajner D, Katanić M, Drekić M, Stojnić S. Restoration of autochthonous poplar species (Populus sp.) in riparian zone – genofond establishment. Topola (Poplar). 2010;185/186:61-8. Serbian.

Castiglione S, Franchin C, Fossati T, Lingua G, Torrigiani P, Biondi S. High zinc concentrations reduce rooting capacity and alter metallothionein gene expression in white poplar (Populus alba L. cv. Villafranca). Chemosphere. 2007;67:1117-26.

Rani V, Raina SN. Genetic fidelity of organized meristem-derived micropropagated plants: A critical reappraisal. In Vitro Cell Dev Biol Plant. 2000;36:319-30.

Confalonieri M, Balestrazzi A, Bisoffi S, Carbonera D. In vitro culture and genetic engineering of Populus spp.: synergy for forest tree improvement. Plant Cell Tissue Org Cult. 2003;72:109-38.

Ahuja MR. A commercially feasible micropropagation method for aspen. Silvae Genetica. 1984;32:174-6.

Kovačević B, Miladinović D, Katanić M, Tomović Z, Pekeč S. The effect of low initial me¬dium pH on in vitro white poplar growth. Bull Fac Forest. 2013;108:67-80.

Skirvin RM, Chu MC, Mann ML, Young H, Sullivan J, Fermanian T. Stability of tissue culture medium pH as a function of autoclaving, time and cultured plant material. Plant Cell Rep. 1986;5:292-94.

Wettstein D. Chlorophyll-letale und der submikroskopische Formwechsel der Plastiden. Exp Cell Res. 1957;12:427-506.

R Development Core Team. R: A language and environment for statistical computing. Vienna: R Foundation for Statistical Computing; 2008. 3501 p.

Do Nascimento CWA, Amarasiriwardena D, Xing B. Comparison of natural organic acids and synthetic chelates at enhancing phytoextraction of metals from a multi-metal contaminated soil. Environ Pollut. 2006;140:114-23.

Evangelou MWH, Ebel M, Schaeffer A. Chelate assisted phytoextraction of heavy metals from soil. Effect, mechanism, toxicity, and fate of chelating agents. Chemosphere. 2007;68:989-1003.

Chen YX, Lin Q, Luo YM, He YF, Zhen SJ, Yu YL, Tian GM, Wong MH. The role of citric acid on the phytoremediation of heavy metal contaminated soil. Chemosphere. 2002;50:807-11.

Maksymiec W. Signaling responses in plants to heavy metal stress. Acta Physiol Plant. 2007;29:177-87.

Watson C, Pulford ID, Riddell-Black D. Screening of willow species for resistance to heavy metals: Comparison of performance in a hydroponics system and field trials. Int J Phytoremediat. 2003;5:351-65.

Pulford ID, Riddell-Black D, Stewart C. Heavy metal uptake by willow clones from sewage sludge-treated soil: the potential for phytoremediation. Int J Phytoremediat. 2002;4:59-72.

Doran PM. Application of Plant Tissue Cultures in Phytoremediation Research: Incentives and Limitations. Biotechnol Bioeng. 2009;103:60-76.

Capuana M. Heavy metals and woody plants - biotechnologies for phytoremediation. iForest. 2011;4:7-15.

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Published

2017-10-18

How to Cite

1.
Vuksanović V, Kovačević B, Katanić M, Orlović S, Miladinović D. In vitro Evaluation of Copper Tolerance and Accumulation in Populus nigra. Arch Biol Sci [Internet]. 2017Oct.18 [cited 2024Apr.19];69(4):679-87. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/1463

Issue

Vol. 69 No. 4 (2017)

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Articles

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Authors grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.

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