Ultrastructural evaluation of oocyte envelopes of zebrafish (Danio rerio) (Hamilton, 1822) after TiO2 nanoparticle exposure

Authors

  • Cansu Akbulut Sakarya University, Department of Biology, Sakarya
  • Tuğba Kotil İstanbul University, İstanbul Faculty of Medicine, Histology and Embryology Department, Istanbul
  • Nazan Deniz Yön Sakarya University, Department of Biology, Sakarya

Keywords:

titanium dioxide (TiO2) nanoparticle, ultrastructure, histopathology, oocyte, zebrafish

Abstract

Titanium dioxide (TiO2)is one of the most widely used nanoparticles, and aquatic organisms are especially exposed to it. To examine reproductive toxicology, zebrafish were exposed to different concentrations (1, 2 and 4 mg/L) of TiO2 nanoparticles. The ultrastructure of the theca cell, zona radiata structure and follicular epithelium were examined in detail by transmission electron microscopy (TEM). No abnormalities were observed in the control group; however, degeneration of pore and microvilli structures of the zona radiata, vacuolization in the ooplasm, mitochondrial swelling and mitotic catastrophe (the mechanism for eliminating mitosis-incompetent cells in eukaryotes) were detected in the exposure groups. These results indicate that TiO2 nanoparticle exposure causes paraptotic-type cell death in zebrafish oocytes, follicular and theca cells. In light of the observed histopathological changes, it was concluded that TiO2 exposure inhibited oogenesis and the reproductive capability in zebrafish.

https://doi.org/10.2298/ABS170303035A

Received: March 3, 2017; Revised: August 8, 2017; Accepted: September 20, 2017; Published online: October 3, 2017

How to cite this article: Akbulut C, Kotil T, Yön DN. Ultrastructural evaluation of oocyte envelopes of zebrafish (Danio Rerio) (Hamilton, 1822) after TiO2 nanoparticle exposure. Arch Biol Sci. 2018;70(1):159-65.

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References

Dayal N, Singh D, Patil P, Thakur M, Vanage G, Joshi DS. Effect of bioaccumulation of gold nanoparticles on ovarian morphology of female Zebrafish (Danio rerio). World J Pathol. 2017;6:1.

Schrand AM, Rahman MF, Hussain SM, Schlager JJ, Smith DA, Syed AF. Metal-based nanoparticles and their toxicity assessment. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010;2(5):544-68.

Wang J, Zhu X, Zhang X, Zhao Z, Liu H, George R, Wilson-Rawls J, Chang Y, Chen Y. Disruption of zebrafish (Danio rerio) reproduction upon chronic exposure to TiO2 nanoparticles. Chemosphere. 2011;83(4):461-7.

Monteiro-Riviere NA, Wiench K, Landsiedel R, Schulte S, Inman AO, Riviere JE. Safety evaluation of sunscreen formulations containing titanium dioxide and zinc oxide nanoparticles in UVB sunburned skin: an in vitro and in vivo study. Toxicol Sci. 2011;123(1):264-80.

Gamer AO, Leibold E, van Ravenzwaay B. The in vitro absorption of microfine zinc oxide and titanium dioxide through porcine skin. Toxicol In Vitro. 2006;20(3):301-7.

Aitken RJ, Chaudhry MQ, Boxall ABA, Hull M. Manufacture and use of nanomaterials: current status in the UK and global trends. Occup Med. 2006;56:300-6.

Taylor U, Tiedemann D, Rehbock C, Kues WA, Barcikowski S, Rath D. Influence of gold, silver and gold-silver alloy nanoparticles on germ cell function and embryo development. Beilstein J Nanotechnol. 2015;6:651-64.

Çağlar AB, Saral S. Kozmetolojide Toksisite Sorunu. Turk J Dermatol. 2014;4:248-51.

Hanna SK, Miller RJ, Muller EB, Nisbet RM, Lenihan HS. Impact of Engineered Zinc Oxide Nanoparticles on the Individual Performance of Mytilus galloprovincialis. Plos One. 2013;8(4):e61800.

Hao LH, Chen L, Hao JM, Zhong N. Bioaccumulation and sub-acute toxicity of zinc oxide nanoparticles in juvenile carp (Cyprinus carpio): A comparative study with its bulk counterparts. Ecotoxicol Environ Safety 2013;91:52-60.

Pinheiro T, Moita L, Silva L, Mendonca E, Picado A. Nuclear microscopy as a tool in TiO2 nanoparticles bioaccumulation studies in aquatic species. Nucl Instrum Methods Phys Res B. 2013;306:117-20.

Ramsden CS, Henry TB, Handy RD. Sub-lethal effects of titanium dioxide nanoparticles on the physiology and reproduction of zebrafish. Aquat Toxicol. 2013;126:404-13.

Bourrachot S, Brion F, Pereira S, Floriani M, Camilleri V, Cavalié I, Palluel O, Adam-Guillermin C. Effects of depleted uranium on the reproductive success and F1 generation survival of zebrafish (Danio rerio). Aquat Toxicol. 2014;54:1-11.

Tsukue N, Tsubone H, Suzuki AK. Diesel exhaust affects the abnormal delivery in pregnant mice and the growth of their young. Inhal Toxicol. 2002;14:635-51.

Zhu RR, Wang SL, Chao J, Shi DL, Zhang R, Sun XY, Yao SD. Bio-effects of nano-TiO2 on DNA and cellular ultrastructure with different polymorph and size. Mater Sci Eng C. 2009;29:691-6.

Kim HR, Park YJ, Shin DY, Oh SM, Chung KH. Appropriate In Vitro methods for Genotoxicity testing of silver Nanoparticles. Environ Health Toxicol. 2013;28:e2013003.

Shi H, Magave R, Castranova V, Zhao J. Titanium dioxide nanoparticles: a review of current toxicological data. Part Fibre Toxicol. 2013;10:15.

Park HM, Yeo MK. Effects of TiO2 nanoparticles and nanotubes on zebrafish caudal fin regeneration. Mol Cell Toxicol. 2013;9(4):375-83.

Komatsu T, Tabata M, Kubo-Irie M, Shimizu T, Suzuki K, Nihei Y, Takeda K. The effects of nanoparticles on mouse testis Leydig cells in vitro. Toxicol In Vitro. 2008;22:1825-31.

Federici G, Shaw BJ, Handy RD. Toxicity of titanium dioxide nanoparticles to rainbow trout (Oncorhynchus mykiss): Gill injury, oxidative stress, and other physiological effects. Aquat Toxicol. 2007;84:415-30.

Prasad RT, Wallace K, Daniel KM, Tennant AH, Zucker RM, Strickland J, Dreher K,. Kligerman AD, Blackman CF, DeMarini DM. Effect of Treatment Media on the Agglomeration of Titanium Dioxide Nanoparticles: Impact on Genotoxicity, Cellular Interaction, and Cell Cycle. ACS Nano. 2013;7(3):1929-42.

Tiedemann D, Taylor U, Rehbock C, Jakobi J, Klein S, Kues WA, Barcikowski S, Rath D. Reprotoxicity of gold, silver, and gold–silver alloy nanoparticles on mammalian gametes. Analyst. 2014;139(5):931-42.

Suganthi P, Murali M, Sadiq-Bukhari A, Syed-Mohamed HE, Basu H, Singhal RK. Behavioural and Histological variations in Oreochromis mossambicus after exposure to ZnO Nanoparticles. Int J Appl Res. 2015;1(8):524-31.

Rather MA, Sharma R, Gupta S, Ferosekhan S, Ramya VL, Jadhao SB. Chitosan-Nanoconjugated Hormone Nanoparticles for Sustained Surge of Gonadotropins and Enhanced Reproductive Output in Female Fish. Plos One. 2013;8(2):e57094.

Chen SX, Yang XY, Deng Y, Huang J, Li Y, Sun Q, Yu C, Zhu Y, Hong WS. Silver nanoparticles induce oocyte maturation in zebrafish (Danio rerio). Chemosphere. 2017;170:51-60.

Sperandio S, deBelle I, Bredesen DE. An alternative, nonapoptotic form of programmed cell death. Proc Natl Acad Sci USA. 2000;97:14376-81.

Danaila L, Popescu I, Pais V, Riga D, Riga S, Pais E. Apoptosis, paraptosis, necrosis, and cell regeneration in posttraumatic cerebral arteries. Chirurgia (Bucur). 2013;108(3):319-24.

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Published

2018-03-13

How to Cite

1.
Akbulut C, Kotil T, Yön ND. Ultrastructural evaluation of oocyte envelopes of zebrafish (Danio rerio) (Hamilton, 1822) after TiO2 nanoparticle exposure. Arch Biol Sci [Internet]. 2018Mar.13 [cited 2024Nov.21];70(1):159-65. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/1568

Issue

Vol. 70 No. 1 (2018)

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Articles

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