Ultrastructural evaluation of oocyte envelopes of zebrafish (Danio rerio) (Hamilton, 1822) after TiO2 nanoparticle exposure

Authors

  • Cansu Akbulut Sakarya University, Department of Biology, Sakarya
  • Tuğba Kotil İstanbul University, İstanbul Faculty of Medicine, Histology and Embryology Department, Istanbul
  • Nazan Deniz Yön Sakarya University, Department of Biology, Sakarya

Keywords:

titanium dioxide (TiO2) nanoparticle, ultrastructure, histopathology, oocyte, zebrafish

Abstract

Titanium dioxide (TiO2)is one of the most widely used nanoparticles, and aquatic organisms are especially exposed to it. To examine reproductive toxicology, zebrafish were exposed to different concentrations (1, 2 and 4 mg/L) of TiO2 nanoparticles. The ultrastructure of the theca cell, zona radiata structure and follicular epithelium were examined in detail by transmission electron microscopy (TEM). No abnormalities were observed in the control group; however, degeneration of pore and microvilli structures of the zona radiata, vacuolization in the ooplasm, mitochondrial swelling and mitotic catastrophe (the mechanism for eliminating mitosis-incompetent cells in eukaryotes) were detected in the exposure groups. These results indicate that TiO2 nanoparticle exposure causes paraptotic-type cell death in zebrafish oocytes, follicular and theca cells. In light of the observed histopathological changes, it was concluded that TiO2 exposure inhibited oogenesis and the reproductive capability in zebrafish.

https://doi.org/10.2298/ABS170303035A

Received: March 3, 2017; Revised: August 8, 2017; Accepted: September 20, 2017; Published online: October 3, 2017

How to cite this article: Akbulut C, Kotil T, Yön DN. Ultrastructural evaluation of oocyte envelopes of zebrafish (Danio Rerio) (Hamilton, 1822) after TiO2 nanoparticle exposure. Arch Biol Sci. 2018;70(1):159-65.

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Published

2018-03-13

How to Cite

1.
Akbulut C, Kotil T, Yön ND. Ultrastructural evaluation of oocyte envelopes of zebrafish (Danio rerio) (Hamilton, 1822) after TiO2 nanoparticle exposure. Arch Biol Sci [Internet]. 2018Mar.13 [cited 2024Dec.22];70(1):159-65. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/1568

Issue

Vol. 70 No. 1 (2018)

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Articles

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