All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells

Authors

  • Jelena Marjanović Vićentić Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO BOX 23, 11010 Belgrade
  • Marija Schwirtlich Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO BOX 23, 11010 Belgrade
  • Nataša Kovačević-Grujičić Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO BOX 23, 11010 Belgrade
  • Milena Stevanović 1. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO BOX 23, 11010 Belgrade; 2. University of Belgrade, Faculty of Biology, Studentski trg 16, 11000 Belgrade; 3. Serbian Academy of Sciences and Arts, Knez Mihailova 35, 11001 Belgrade
  • Danijela Drakulić Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, PO BOX 23, 11010 Belgrade

Keywords:

glioblastoma, ATRA, differentiation, viability, cell migration

Abstract

Glioblastoma (GBM) is one of the most aggressive and deadly forms of cancer. Literature data reveals that all-trans retinoic acid (ATRA) has anticancer effects on different types of tumor cells. However, data about the effects of ATRA on glioblastoma cells are contradictory. In this study, we examined whether ATRA treatment affects features of human glioblastoma U251 cells. To that end, the cells were treated with different concentrations of ATRA. Results obtained by MTT and the crystal violet assays imply that ATRA affected the viability of U251 glioblastoma cells in a dose- and time-dependent manner. Fluorescence staining of microtubule cytoskeleton protein α-tubulin revealed that ATRA induced changes in cell morphology. Using semi-quantitative RT-PCR we found that the expression of SOX3 and GFAP genes, as markers of neural differentiation, was not changed upon ATRA treatment. Thus, the observed changes in cell morphology after ATRA treatment are not associated with neural differentiation of U251 glioblastoma cells. The scratch-wound healing assay revealed that ATRA changed the mode of U251 cell migration from collective to single cell motility. The cell-matrix adhesion assay demonstrated that the pharmacologically relevant concentration of ATRA lowered the cell-matrix adhesion capability of U251 cells. In conclusion, our results imply that further studies are needed before ATRA could be considered for the treatment of glioblastoma.

https://doi.org/10.2298/ABS170327016M

Received: March 27, 2017; Revised: May 23, 2017; Accepted: June 2, 2017; Published online: June 12, 2017

How to cite this article: Marjanović Vićentić J, Schwirtlich M, Kovačević-Grujičić N, Stevanović M, Drakulić D. All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells. Arch Biol Sci. 2017;69(4):699-706.

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Published

2017-10-18

How to Cite

1.
Marjanović Vićentić J, Schwirtlich M, Kovačević-Grujičić N, Stevanović M, Drakulić D. All-trans retinoic acid influences viability, migration and adhesion of U251 glioblastoma cells. Arch Biol Sci [Internet]. 2017Oct.18 [cited 2024Nov.24];69(4):699-706. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/1617

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Vol. 69 No. 4 (2017)

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