Prevention of diet-induced obesity in rats by oral application of collagen fragments

Authors

  • Nataliia G. Raksha Educational and Scientific Center “Institute of Biology and Medicine” Taras Shevchenko National University of Kyiv
  • Pavlo Y. Potalitsyn Educational and Scientific Center “Institute of Biology and Medicine” Taras Shevchenko National University of Kyiv
  • Alona V. Yurchenko Educational and Scientific Center “Institute of Biology and Medicine” Taras Shevchenko National University of Kyiv
  • Tetyana I. Halenova Educational and Scientific Center “Institute of Biology and Medicine” Taras Shevchenko National University of Kyiv
  • Oleksii M. Savchuk Educational and Scientific Center “Institute of Biology and Medicine” Taras Shevchenko National University of Kyiv
  • Lydmila I. Ostapchenko Educational and Scientific Center “Institute of Biology and Medicine” Taras Shevchenko National University of Kyiv

Keywords:

collagen fragments, obesity, oxidative stress, inflammation, insulin resistance

Abstract

The aim of the present study was to determine whether orally applied collagen fragments (CFs) could affect the development of obesity in obese rats. To this end, experimental rats that were exposed to a high-calorie diet (HCD) for four weeks were randomly divided into two groups: HCD and HCD+CFs, with both groups continuing to receive the HCD. However, rats from the HCD+CFs group were also provided with CFs in a 0.05-M citrate buffer (pH 5.0) (1 g·kg-1 of body weight) by intragastric administration, every other day for the next six weeks. Selected parameters associated with obesity development and insulin resistance, as well as serum markers of oxidative stress and the cytokine profile were assessed at the end of the 10th week. Supplementation with CFs resulted in a decrease in body weight and body mass index when compared to animals exposed to a HCD. The observed changes were assumed to be caused by a lower food intake and increased water intake by obese rats treated with CFs. Enhanced activity of superoxide dismutase (SOD), catalase (CAT) and decreased malondialdehyde (MDA) concentration were detected in the HCD+CF group of animals when compared to untreated HCD-fed rats. Administration of CFs also lowered the serum concentrations of the proinflammatory cytokines IL-1β and IL-12, whereas the concentration of the anti-inflammatory cytokine IL-10 was significantly increased and the concentration of cytokine IL-4 was near the control value. Decreased concentrations of fasting blood glucose, glycated hemoglobin (GHbA1c) and serum insulin and increased tolerance to glucose in the oral glucose tolerance test (OGTT) were observed in the HCD+CF group of animals when compared to rats in the HCD group. We concluded that CFs mediated a therapeutic effect on obesity development in rats exposed to a HCD by affecting pathways involved in obesity pathogenesis.

https://doi.org/10.2298/ABS170401027R

Received: April 1, 2017; Revised: June 1, 2017; Accepted: June 13, 2017; Published online: August 1, 2017

How to cite this article: Raksha NG, Potalitsyn PY, Yurchenko AV, Halenova TI, Savchuk OM, Ostapchenko LI. Prevention of diet-induced obesity in rats by oral application of collagen fragments. Arch Biol Sci. 2018;70(1):77-86.

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References

Hossain P, Kawar B, Nahas M. Obesity and diabetes in the developing world - a growing challenge. N Engl J Med. 2007;356(3):213-5.

Reaven G.M. Insulin resistance: the link between obesity and cardiovascular disease. Endocrinol Metab Clin North Am. 2008;37:581-601.

Mokdad AH, Ford ES, Bowman BA, Dietz WH, Vinicor F, Bales VS, Marks JS. Prevalence of obesity, diabetes, and obesity-related health risk factors, 2001. JAMA. 2003;289:76-9.

Aneiros A, Garateix A. Bioactive peptides from marine sources: pharmacological properties and isolation procedures. J Chromatogr B Analyt Technol Biomed Life Sci. 2004;803(1):41-53.

Ngo D-H, Vo T-S, Ngo D-N, Wijesekara I, Kim S-K. Biological activities and potential health benefits of bioactive peptides derived from marine organisms. Int J Biol Macromol. 2012;51:378-83.

Harnedy PA, Richard J. FitzGerald RJ. Bioactive peptides from marine processing waste and shellfish: A review. J Func Foods. 2012;4:6-24.

Kim S-K, Mendis E. Bioactive compounds from marine processing byproducts - A review. Food Res Int. 2006;39:383-93.

Nagai T, Izumi M, Ishii M. Fish scale collagen. Preparation and partial characterization. Int J Food Sci Tech. 2004;39:239-44.

Wang H, Fu Z, Han C. The potential applications of marine bioactives against diabetes and obesity. American J Marine Sci. 2014;2(1):1-8.

Zhang H, Dong Y, Qi B, Liu L, Zhou G, Bai X, Yang Ch, Zhao D, Zhao Y. Preventive effects of collagen peptide from deer sinew on bone loss in ovariectomized rats. Evid Based Complement Alternat Med. 2014;2014:627285.

Wang B, Wang YM, Chi CF, Luo HY, Deng SG, Ma JY. Isolation and characterization of collagen and antioxidant collagen peptides from scales of croceine croaker (Pseudosciaena crocea). Marine Drugs. 2013;11(11):4641-61.

Zhu C-F, Li G-Z, Peng H-B, Zhang F, Chen Y, Li Y. Treatment with marine collagen peptides modulates glucose and lipid metabolism in Chinese patients with type 2 diabetes mellitus. Appl Physiol Nutr Metab. 2010;35:797-804.

Furukawa S, Takuya Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, Nakayama O, Makishima M, Morihiro M, Shimomura I. Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest. 2004;114:1752-61.

Xu H, Barnes GT, Yang Q, Tan G, Yang D, Chou CJ, Sole J, Nichols A, Ross JS, Tartaglia LA, Chen H. Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance. J Clin Investig. 2003;112:1821-30.

Zhang F, Wang A, Li Z, He S, Shao L. Preparation and characterisation of collagen from freshwater fish scales. Food Nutr Scie. 2011;2:818-23.

Huang Q, Li S, Teng H, Jin Y, Ma M, Song H. Optimizing preparation conditions for angiotensin-I-converting enzyme inhibitory peptides derived from enzymatic hydrolysates of ovalbumin. Food Sci Biotechnol. 2015;24(6):2193-98.

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680-85.

Shen X-H, Tang Q-Y, Huang J, Cai W. Vitamin E regulates adipocytokine expression in a rat model of dietary-induced obesity. Exp Biol Med. 2010;235:47-51.

Bradford MM. A rаpid and sensitive method for quantities of utilizing the principle of protein binding. Anal Biochem. 1976;86:193-200.

Nedzvetsky V, Andrievsky G, Chachibaia T, Tykhomyrov A. Differences in antioxidant/protective efficacy of hydrated C60 fullerene nanostructures in liver and brain of rats with streptozotocin-induced diabetes. J Diabetes Metab. 2012;3(8):1-9.

Sirota TV. A novel approach to study the reaction of adrenaline autooxidation: A possibility for polarographic determination of superoxide dismutase activity and antioxidant properties of various preparations. Biochem (Moscow) Suppl Series B: Biomed Chem. 2011;5(3):253-59.

Korolyuk MA, Ivanov LI, Mayorova IG, Tokarev VE. Method for determining the activity of catalase. Lab Delo.1988;1:16-9.

Halenova TI, Vareniuk IM, Roslova NM, Dzerzhynsky ME, Savchuk OM, Ostapchenko LI, Prylutskyy YuI, Ritter U, Scharff P. Hepatoprotective effect of orally applied water-soluble pristine C60 fullerene against CCl4-induced acute liver injury in rats. RSC Adv. 2016;6:100046-55.

Aoyama T, Fukui K, Nakamori T, Hashimoto Y, Yamamoto T, Takamatsu K. Effect of soy and milk whey protein isolates and their hydrolysates on weight reduction in genetically obese mice. Biosci Biotech Biochem. 2000;64:2594-00.

Karpovets TP, Konopelnyuk VV, Savchuk OM, Ostapchenko LI. Food behavior of rats under development of obesity. Res J Pharmaceut Biol Chem Sci. 2014;5(5):253-9.

Nоvеllі Е, Dіnіz Y, Gаlhаrdі C. Аnthrоpоmеtrіcаl pаrаmеtеrsаnd mаrkеrsоf оbеsіtyіn rаts. Lаb Аnіmаls. 2007;41:111-19.

Sеаlе P, Lаzаr M. Brоwn fаt іn humаns: turnіng up thе hеаt оn obеsіty. Dіаbеtеs. 2009;58(7):1482-4.

Moran TH. Cholecystokinin and satiety: current perspectives. Nutr. 2000; 16(10):858-65.

Higdon J, Frei B. Obesity and oxidative stress: A direct link to CVD? Arterioscler Tromb Vasc. Biol. 2003;23:365-67.

Sarmadi BH, Ismail A. Antioxidative peptides from food proteins: A review. Peptides. 2010;31:1949-56.

Qian ZJ, Jung WK, Byun HG, Kim SK. Protective effect of an antioxidative peptide purified from gastrointestinal digests of oyster, Crassostrea gigas against free radical induced DNA damage. Biores Technol. 2008;99:3365-71.

Mendis E, Rajapakse N, Byun HG, Kim SK. Investigation of jumbo squid (Dosidicus gigas) skin gelatin peptides for their in vitro antioxidant effects. Life Sci. 2005;77:2166-78.

Ranathunga S, Rajapakse N, Kim S-K. Purification and characterization of antioxidative peptide derived from muscle of conger eel (Conger myriaster). Eur Food Res Technol. 2006;222:310-5.

Grosser N, Oberle S, Berndt G, Erdmann K, Hemmerle A, Schröder H. Antioxidant action of L-alanine: heme oxygenase-1 and ferritin as possible mediators. Biochem Biophys Res Commun. 2004;314(2):351-5.

Himaya SW, Ryu B, Ngo DH, Kim SK. Peptide isolated from Japanese flounder skin gelatin protects against cellular oxidative damage. J Agric Food Chem. 2012;60:9112-9.

Kang JH. Modification and inactivation of Cu,Zn-superoxide dismutase by the lipid peroxidation product, acrolein. BMB Rep. 2013;46(11):555-60.

Ishizaki-Koizumi S, Sonaka I, Fujitani S, Nishiguchi S. Mechanisms of the protective effect of L-alanine to D-galactosamine-induced hepatocellular injury: comparative studies of L-alanine and pyruvate. Biochem Biophys Res Commun. 2002;291:738-43.

Lin L, Li B. Radical scavenging properties of protein hydrolysates from Jumbo flying squid (Dosidicus eschrichitii Steenstrup) skin gelatin. J Sci Food Agric. 2006;86:2290-5.

Liu GQ, Zhu CF, Li YF, Peng HB, Lu H, Guo SC. Effect of marine collagen peptide on the expression of overoxidation stress markers in rats with type 2 diabetes. J Clin Rehabil Tissue Eng Res. 2008;12:4469-72.

Lin B, Zhang F, Yu Y, Jiang Q, Zhang Z, Wang J, Li Y. Marine collagen peptides protect against early alcoholic liver injury in rats. British J Nutr. 2012;107:1160-6.

Stienstra R, Tack CJ, Kanneganti TD, Joosten LA, Netea MG. The inflammasome puts obesity in the danger zone. Cell Metab. 2012;15:10-8.

McArdle MA, Finucane OM, Connaughton RM, McMorrow AM, Roche HM. Mechanisms of obesity-induced inflammation and insulin resistance: insights into the emerging role of nutritional strategies. Front Endocrinol. 2013;4:1-23.

Fonseca-Alaniz MH, Takada J, Alonso-Vale MI, Lima FB. Adipose tissue as an endocrine organ: From theory to practice. J Pediatr. 2007;83:192-203.

Rasouli N, Kern PA. Adipocytokines and the metabolic complications of obesity. J Clin Endocrinol Metab. 2008;93(1):S64-S73.

Ahn CB, Cho YS, Je JY. Purification and anti-inflammatory action of tripeptide from salmon pectoral fin byproduct protein hydrolysate. Food Chem. 2015;168:151-6.

Kahn SE, Hull RL, Utzschneider KM. Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature. 2006;444:840-6.

Kahn BB, Flier JS. Obesity and insulin resistance. J Clin Invest. 2000;106(4):473-81.

Ouellet V, Marois J, Weisnagel SJ, Jacques H. Dietary cod protein improves insulin sensitivity in insulin-resistant men and women: a randomized controlled trial. Diabetes Care. 2007; 30:2816-21.

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Published

2018-03-13

How to Cite

1.
Raksha NG, Potalitsyn PY, Yurchenko AV, Halenova TI, Savchuk OM, Ostapchenko LI. Prevention of diet-induced obesity in rats by oral application of collagen fragments. Arch Biol Sci [Internet]. 2018Mar.13 [cited 2024Dec.22];70(1):077-86. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/1636

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Vol. 70 No. 1 (2018)

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