Maize seedling performance as a potential index for drought tolerance
Keywords:
drought, grain yield, POD activity, proline content, Zea mays L.Abstract
Twenty-six maize landraces were tested in order to evaluate maize seedling performance as an index for drought tolerance in adult plants. Samples were subjected to polyethylene glycol-induced osmotic stress at the early seedling stage. Grain yield was obtained in field experiments under well-watered (OC) and a combination of drought and high plant density (HD) conditions. Osmotic stress caused a reduction in seedling growth (length, fresh and dry weight), and increases in the shoot and in particular the root proline contents in the majority of landraces, and variations in root peroxidase (POD) activity. Genotypes displaying more pronounced root growth reduction and higher proline contents exhibited decreased POD activity under osmotic stress. Direct positive correlations between the proline content and growth inhibition, and between the proline and soluble protein content were established. Correlations between the changes in POD activity and growth parameters were significant and positive, and significant but negative with the changes in the proline content. In the field, water stress led to a reduction in grain yield in all of the tested landraces. Correlations between grain yield from both experimental sets (OC and HD) and osmotic-induced changes in seedling root growth were negative, which was opposite to the highly significant and positive correlations between the changes in the seedling root proline content and yield. Also, genotypes with the highest seedling root proline content increase under osmotic stress, exhibited the highest stress tolerance index (STI) based on grain yield achieved under both field conditions. Our results indicate that lower changes in POD activity and especially an increased proline content after exposure to osmotic stress during the early seedling stage could be considered as useful indices to facilitate selection efficiency for drought tolerance in adult plants.
https://doi.org/10.2298/ABS170504036K
Received: May 4, 2017; Revised: August 31, 2017; Accepted: September 28, 2017; Published online: October 6, 2017
How to cite this article: Kravić N, Hadži-Tašković Šukalović V, Babić V, Srdić J, Mesarović J, Anđelković V. Maize seedling performance as a potential index for drought tolerance. Arch Biol Sci. 2018;70(1):167-77.
Downloads
References
Hirayama T, Shinozaki K. Research on plant abiotic stress responses in the post-genome era: past, present and future. Plant J. 2010;61:1041-52.
Foyer CH, Noctor G. Redox homeostasis and antioxidant signaling: a metabolic interface between perception and physiological responses. Plant Cell. 2005;17:1866-75.
Sharma P, Dubey RS. Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regul. 2005;46:209-21.
Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 2002;7:405-10.
Hadži-Tašković Šukalović V, Vuletić M, Marković K, Vučinić Z, Kravić N. Modification of antioxidant systems in cell walls of maize roots by different nitrogen sources. Span J Agric Res. 2016;14:e0808.
Kavi Kishor PB, Sangam S, Amrutha RN, Sri Laxmi P, Naidu KR, Rao KRSS, Reddy KJ, Theriappan P, Sreenivaslu N. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Curr Sci. 2005;88:424-38.
Szabados L, Savouré A. Proline: a multifunctional amino acid. Trends Plant Sci. 2010;15:89-97.
Matysik J, Alia A, Bhalu B, Mohanty P. Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Curr Sci. 2002;82:525-32.
Csiszár J, Gallé A, Horváth E, Dancsó P, Gombos M, Váry Z, Erdei L, Györgyey J, Tari I. Different peroxidase activities and expression of abiotic stress-related peroxidases in apical root segments of wheat genotypes with different drought stress tolerance under osmotic stress. Plant Physiol Biochem. 2012;52:119-29.
Passardi F, Cosio C, Penel C, Dunand C. Peroxidases have more functions than a Swiss army knife. Plant Cell Rep. 2005;24:255-65.
Liszkay A, van der Zalm E, Schopfer P. Production of reactive oxygen intermediates (O2-, H2O2, OH) by maize roots and their role in wall loosening and elongation growth. Plant Physiol. 2004;136:3114-23.
Cosio C, Dunand C. Specific functions of individual class III peroxidase gene. J Exp Bot. 2009;60:391-408.
Wu GQ, Zhang LN, Wang YY. Response of growth and antioxidant enzymes to osmotic stress in two different wheat (Triticum aestivum L.) cultivars seedlings. Plant Soil Environ. 2012;58:534-9.
Bibi A, Sadaqat HA, Tahir MHN, Akram HM. Screening of sorghum (Sorghum bicolor Var Moench) for drought tolerance at seedling stage in polyethylene glycol. J Anim Plant Sci. 2012;22:671-8.
Valentovič P, Luxová M, Kolarovič L, Gašparíková O. Effect of osmotic stress on compatible solutes content, membrane stability and water relations in two maize cultivars. Plant Soil Environ. 2006;52:186-91.
Tuberosa R, Sanguineti MC, Landi P, Giuliani MM, S. Salvi S, Conti S. Identification of QTLs for root characteristics in maize grown in hydroponics and analysis of their overlap with QTLs for grain yield in the field at two water regimes. Plant Mol Biol. 2002;48:697-712.
Landi P, Giuliani MM, Darrah LL, Tuberosa R, Conti S, Sanguineti MC. Variability for root and shoot traits in a maize population grown in hydroponics and in the field and their relationships with vertical root pulling resistance. Maydica. 2001;46:177-82.
Dodig D, Zorić M, Jović M, Kandić V, Stanisavljević R, Šurlan-Momirović G. Wheat seedlings growth response to water deficiency and how it correlates with adult plant tolerance to drought. J Agr Sci. 2015;153:466-80.
Hadži-Tašković Šukalović V, Vuletić M, Vučinić Ž. The role of p-coumaric acid in oxidative and peroxidative cycle of the ionically bound peroxidase of the maize root cell wall. Plant Sci. 2005;168:931-8.
Bates LS, Waldren SP, Teare ID. Rapid determination of free proline for water-stress studies. Plant Soil. 1973;39:205-7.
Lowry OH, Rosebrogh NJ, Farr AL, Randal RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265-75.
Akçura M, Partigoç F, Kaya Y. Evaluating of drought stress tolerance based on selection indices in Turkish bread wheat landraces. J Anim Plant Sci. 2011;21:700-9.
Grzesiak MT, Waligórski P, Janowiak F, Marcińska I, Hura K, Szczyrek P, Głąb T. The relations between drought susceptibility index based on grain yield (DSIGY) and key physiological seedling traits in maize and triticale genotypes. Acta Physiol Plant. 2013;35:549-65.
Sharp RE, Poroyko V, Hejlek LG, Spollen WG, Springer GK, Bohnert HJ, Nguyen HT. Root growth maintenance during water deficits; physiology to functional genomics. J Exp Bot. 2004;55:2343-51.
Wiegers BS, Cheer AY, Silk WK. Modeling the Hydraulics of Root Growth in Three Dimensions with Phloem Wates Sources. Plant Physiol. 2009;150:2092-103.
Lopes MS, Araus JL, van Heerden PDR, Foyer CH. Enhancing drought tolerance in C4 crops. J Exp Bot. 2011; 62:3135-53.
Bruce WB, Edmeades GO, Barker TC. Molecular and physiological approaches to maize improvement for drought tolerance. J Exp Bot. 2002;53:13-25.
Yang SL, Lan SS, Gong M. Hydrogen peroxide-induced proline and metabolic pathway of its accumulation in maize seedlings. J Plant Physiol. 2009;166:1694-9.
Claussen W. Proline as a measure of stress in tomato plants. Plant Sci. 2005;168:241-8.
Mwandzingeni L, Shimelis H, Tesfay S, Tsilo TJ. Screening of bread wheat genotypes for drought tolerance using phenotypic and proline analyses. Front Plant Sci. 2016;7:1276.
Takahama U. Oxidation of vacuolar and apoplastic phenolic substrates by peroxidase: physiological significance of the oxidation reactions. Phytochem Rev.2004;3:207-19.
Ralph J. Hydroxycinnamates in lignification. Phytochem Rev. 2010;9:65-83.
Vuletić M, Hadži-Tašković Šukalović V, Marković K, Dragišić Masimović J. Antioxidative system in maize roots as affected by osmotic stress and different nitrogen sources. Biol Plant. 2010;54:530-4.
Kravić N, Marković K, Anđelković V, Hadži-Tašković Šukalović V, Babić V, Vuletić M. Growth, proline accumulation and peroxidase activity in maize seedlings under osmotic stress. Acta Physiol Plant. 2013;35:233-9.
Fazeli F, Ghorbanli M, Niknam V. Effect of drought on biomass, protein content, lipid peroxidation and antioxidant enzymes in two sesame cultivars. Biol Plant. 2007;1:98-103.
Polesskaya OG, Kashirina EI, Alekhina ND. Changes in the activity of antioxidant enzymes in wheat leaves and roots as a function of nitrogen source and supply. Russ J Plant Physiol. 2004;51:615-20.
Vuletić M, Hadži-Tasković Šukalović V, Marković K, Kravić N, Vučinić Ž, Maksimović V. Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress. Plant Biol. 2014;16:88-96.
Singhal NC, Mehta SL, Sing MP. Peroxidase activities in relation to plant height and grain weight in bread wheat (Triticum aestivum L.). Theor Appl Genet.1979;55:87-92.
Moran JF, Becana M, Iturbe-Ormaetxe I, Frechilla S, Klucas RV, Aparicio-Tejo P. Drought induces oxidative stress in pea plants. Planta. 1994;194:346-52.
Egert M, Tevini M. Influence of drought on some physiological parameters symptomatic for oxidative stress in leaves of chives (Allium schoenoprasum). Environ Exper Bot. 2002;48:43-9.
Liu T, Zhang L, Yuan Z, Hu X, Lu M, Wang W et al. Identification of proteins regulated by ABA in response to combined drought and heat stress in maize roots. Acta Physiol Plant. 2013;35:501-13.
Riccardi F, Gazeau P, Vienne D, Zivy M. Protein changes in response to progressive water deficit in maize, quantitative variation and polypeptide identification. Plant Physiol. 1998;117:1253-63.
Ti-da GE, Fang-Gong-Sui S, Ping B, Yingyan LU, Guang-sheng Z. Effects of water stress on the protective enzymes and lipid peroxidation in roots and leaves of summer maize. Agric Sci China. 2006;5:291-8.
Mafakheri A, Siosemardeh A, Bahramnejad B, Struik PC, Sohrabi Y. Effect of drought stress and subsequent recovery on protein, carbohydrate contents, catalase and peroxidase activities in three chickpea (Cicer arietinum) cultivars. Austral J Crop Sci. 2011;5:1255-60.
Irigoyen JJ, Emerich DW, Sanchez-Diaz M. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol Plant. 1992;84:55-60.
Bänziger M, Edmeades GO, Beck D, Bellon M. Breeding for Drought and Nitrogen Stress Tolerance in Maize: From Theory to Practice. Mexico DF: CIMMYT; 2000. p. 68.
Adebayo MA, Menkir A, Blay E, Gracen V, Danquah E, Hearne S. Genetic analysis of drought tolerance in adapted x exotic crosses of maize inbred lines under managed stress conditions. Euphytica. 2014;196:261-70.
Blum A. Crop responces to drought and the interpretation of adaptation. Plant Growth Reg. 1996;20:135-48.
Vancetovic J, Bozinovic S, Ignjatovic-Micic D, Delic N, Kravic N, Nikolic A. A diallel cross among drought tolerant maize populations. Euphytica. 2015;205:1-16.
Downloads
Published
How to Cite
Issue
Section
License
Authors grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.
