Genome-wide identification and characterization of stress-associated protein (SAP) gene family encoding A20/AN1 zinc-finger proteins in Medicago truncatula

Authors

  • Yong Zhou Department of Biochemistry and Molecular Biology, College of Science, Jiangxi Agricultural University, Nanchang 330045 http://orcid.org/0000-0002-8432-6678
  • Liming Zeng Department of Biochemistry and Molecular Biology, College of Science, Jiangxi Agricultural University, Nanchang 330045 http://orcid.org/0000-0001-8307-626X
  • Rongrong Chen Department of Biochemistry and Molecular Biology, College of Science, Jiangxi Agricultural University, Nanchang 330045 http://orcid.org/0000-0002-4489-4179
  • Yihua Wang Department of Biochemistry and Molecular Biology, College of Science, Jiangxi Agricultural University, Nanchang 330045 http://orcid.org/0000-0003-0993-2210
  • Jianbo Song 1. Department of Biochemistry and Molecular Biology, College of Science, Jiangxi Agricultural University, Nanchang 330045; 2. Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Science, Shenzhen University, Shenzhen 518060 http://orcid.org/0000-0003-0158-2527

Keywords:

Medicago truncatula, stress associated protein (SAP), gene family, expression analysis, abiotic stress

Abstract

Stress associated proteins (SAPs) play important roles in developmental processes, responses to various stresses and hormone stimulation in plants. However, little is known about the SAP gene family in Medicago truncatula. In this study, a total of 17 MtSAP genes encoding A20/AN1 zinc-finger proteins were characterized. Out of these 17 genes, 15 were distributed over all 8 chromosomes at different densities, and two segmental duplication events were detected. The phylogenetic analysis of these proteins and their orthologs from Arabidopsis and rice suggested that they could be classified into five out of the seven groups of SAP family genes, with genes in the same group showing similar structures and conserved domains. The cis-elements of the MtSAP promoters were studied, and many cis-elements related to stress and plant hormone responses were identified. We also investigated the stress-responsive expression patterns of the MtSAP genes under various stresses, including drought, exposure to NaCl and cold. The qRT-PCR results showed that numerous MtSAP genes exhibited transcriptional responses to multiple abiotic stresses. These results lay the foundation for further functional characterization of SAP genes. To the best of our knowledge, this is the first report of a genome-wide analysis of the SAP gene family in Medicago truncatula.

https://doi.org/10.2298/ABS170529028Z

Received: May 29, 2017; Revised: July 28, 2017; Accepted: July 31, 2017; Published online: August 24, 2017

How to cite this article: Zhou Y, Zeng L, Chen R, Wang Y, Song J. Genome-wide identification and characterization of stress-associated protein (SAP) gene family encoding A20/AN1 zinc-finger proteins in Medicago truncatula. Arch Biol Sci. 2018;70(1):87-98.

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Published

2018-03-13

How to Cite

1.
Zhou Y, Zeng L, Chen R, Wang Y, Song J. Genome-wide identification and characterization of stress-associated protein (SAP) gene family encoding A20/AN1 zinc-finger proteins in Medicago truncatula. Arch Biol Sci [Internet]. 2018Mar.13 [cited 2024Nov.21];70(1):087-98. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/1826

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Vol. 70 No. 1 (2018)

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