Molecular identification of Trichogramma egg parasitoids of Ostrinia nubilalis in northeastern Serbia

Authors

  • Aleksandar Ivezić Forecasting and Warning Service of Plant Protection in Serbia, Temerinska street 131, Novi Sad
  • Paul Rugman-Jones Department of Entomology, University of California Riverside, 900 University Ave, Riverside, California
  • Richard Stouthamer Department of Entomology, University of California Riverside, 900 University Ave, Riverside, California
  • Aleksandra Ignjatović-Ćupina Faculty of Agriculture, University of Novi Sad, Dositeja Obradovića square 8, Novi Sad

Keywords:

ITS2, Ostrinia nubilalis, PCR, Trichogramma spp., Wolbachia

Abstract

Paper description:

  • Internal transcribed spacer 2 (ITS2) sequences have proved to be useful in providing reliable identification of Trichogramma species.
  • Identification of resident populations of Trichogramma wasps to species level has not been done in Serbia and has limited the potential for large scale use of these parasitoids.
  • The current study used DNA sequences of the ITS2 gene to identify the most prevalent species in Vojvodina as Trischogramma brassicae.
  • Accurate identification of native populations of Trichogramma wasps should help in the choice of the most suitable species for mass rearing and field release.
 

Abstract: The European corn borer Ostrinia nubilalis (Hübner, 1796) (Lepidoptera: Pyralidae) is a major pest of corn (Zea mays Linnaeus, 1753) in Serbia. Regular monitoring of O. nubilalis in the region of Kikinda (province of Vojvodina, Serbia) demonstrated a high percentage of parasitized eggs of this pest. Preliminary identification of the egg parasitoids based on morphological traits revealed that they belong to the genus Trichogramma Westwood, 1833 (Hymenoptera, Trichogrammatidae). The objective of this study was to identify the autochthonous Trichogramma species present in the region of Kikinda by using sequences of ribosomal DNA. Specimens of Trichogramma were reared from parasitized eggs of O. nubilalis collected at different localities in the region of Kikinda. The ITS2 gene was sequenced and subjected to BLAST searches of GenBank NCBI (National Center for Biotechnology Information). The majority of collected specimens were identified as Trichogramma brassicae Bezdenko, 1968, and a single specimen as T. evanescens Westwood, 1833. Intraspecific variation of T. brassicae was assessed by amplifying the mitochondrial cytochrome oxidase I (COI) gene. Wasps were also tested for the presence of the endosymbiotic bacteria Wolbachia Hertig, 1936 using Wolbachia-specific 16S and wsp primers as a preliminary test for evidence of thelytoky. Understanding of the diversity and biology of autochthonous Trichogramma species is a key step in the potential commercial implementation of these egg parasitoid wasps in the protection of corn fields in Serbia.

https://doi.org/10.2298/ABS171103002I

Received: November 13, 2017; Revised: January 12, 2018; Accepted: January 18, 2018; Published online: January 23, 2018

How to cite this article: Ivezić A, Rugman-Jones P, Stouthamer R, Ignjatović-Ćupina A. Molecular identification of Trichogramma egg parasitoids of Ostrinia nubilalis in northeastern Serbia. Arch Biol Sci. 2018;70(3):…

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References

Ilić I, Jovanović S, Janković-Miličić V. Forecasting corn production in Serbia using Arima model. Econ Agricult. 2016;63(4):1141-56.

Čamprag D, Krnjaić Đ, Maceljski M, Maček J, Marić A, Vrabl S. Priručnik izveštajne i prognozne službe zaštite poljoprivrednih kultura. Beograd: Savez društava za zaštitu bilja Jugoslavije; 1983. 682 p.

Smith SM. Biological control with Trichogramma: advances, successes, and potential of their use. Annu Rev Entomol. 1996;41:375-406.

Van Lenteren JC. Success in biological control of arthropods by augmentation of natural enemies. In: Gurr G, Wratten S, editors. Biological control: measures of success. London: Kluwer Academic Publishers; 2000. p. 77-103.

Hassan SA. Mass-production and utilization of Trichogramma: 2. Four years successful biological control of the European corn borer. Mededelingen van de faculteit Land-bouwwetenschappen Rijksuniversiteit Gent. 1981;46(2):417-427.

Wang B, Ferro DN, Hosmer DW. Effectiveness of Trichogramma ostriniae and T. nubilale for controlling the European corn borer Ostrinia nubilalis in sweet corn. Entomol Exp App. 1999;91:297-303.

Beglyarov GA, Smitnik AI. Seasonal colonization of entomophages in the USSR. In: Ridgway RL, Vinson SB, editors. Biological Control by Augmentation of Natural Enemies. Insect and mite control with parasites and predators. New York: Plenum Press; 1977. p. 283-328.

Hassan SA. The mass rearing and utilization of Trichogramma to control lepidopterous pests: achievements and outlook. Pestic Sci. 1993; 37(4):387-391.

Bigler F. Mass production of Trichogramma maidis Pint. et Voeg. and its field application against Ostrinia nubilalis Hbn. in Switzerland. J App Entomol. 1986;101:23-9.

Van Schelt J, Ravensberg WJ. Some aspects on the storage and application of Trichogramma maidis in corn. In: Wajnberg E, Vinson SB, editors. The Third International Symposium on Trichogramma and other Egg Parasitoids; 1990 Sep 23-27; San Antonio, USA. Paris: Institut nationale de la recherche agronomique; 1991. p. 239-42

Silva IMMS, Honda J, Van Kan F, Hu SJ, Neto L, Pintureau B, Stouthamer R. Molecular differentiation of five Trichogramma species occurring in Portugal. BioControl. 1999;16(2):177-84.

Oztemiz S. Trichogramma species egg parasitoids of Lepidoptera in the eastern Mediterranean region of Turkey. Proc Entomol Soc Wash. 2007;109(3):718-20.

Pintureau B. Systématique évolutive du genre Trichogramma Westwood (Hymenoptera: Trichogrammatidae) en Europe. dissertation]. Paris]: Université Paris VII; 1987. 311 p.

Whitman D, Nordlund DA. Plant chemicals and the location of herbivorous arthropods by their natural enemies. In: Ananthakrishnan TN, editor. Functional Dynamics of Phytophagous Insects. New Delhi: Oxford and IBH Publishing; 1994. p. 207-48.

Stouthamer R, Hu J, Van Kan FJPM, Platner GR, Pinto JD. The utility of internally transcribed spacer 2 DNA sequences of the nuclear ribosomal gene for distinguishing sibling species of Trichogramma. BioControl. 1999;43(4):421-40.

Nagaratti S, Nagaraja H. Biosystematic studies on Trichogramma species: I. Experimental hybridization between Trichogramma austrialicum Girault, T. evanescens Westwood, and T. minutum Riley. Tech Bull. 1968;10:81-96.

Nagarkatti S, Nagaraja H. Redescriptions of some known species of Trichogramma showing the importance of the male genitalia as a diagnostic character. Bull Entl Res. 1971;61(1):13-31.

Pinto JD, Stouthomer R. Systematics of the Trichogrammatide with emphasis on Trichogramma. In: Wajberg E, Hassan SA, editors. Biological control with egg parasitoids. Wallingford: Cab International; 1994. p. 1-36.

Pinto JD, Koopmanschap AB, Platner GR, Stouthamer R. The North American Trichogramma parasitizing certain Tortricidae (Lepidoptera) on apple and pear with ITS2 DNA characterization and description of a new species. Biol Control. 2002;23(2):134-42.

Ciociola AI Jr, Zucchi RA, Stouthamer R. Molecular key to seven Brazilian species of Trichogramma using sequences of the ITS2 region and restriction analysis. Neotrop Entomol. 2001a;30(2):259-62.

Thomson L, Rundle BJ, Carew ME, Hoffmann AA. Identification and characterization of Trichogramma species from south-eastern using the internal transcribed spacer 2 (ITS2) region of the ribosomal gene complex. Entomol Exp App. 2003;106(3):235-40.

Borghuis A, Pinto JD, Platner GR, Stouthamer R. Partial cytochrome oxidase II sequences distinguish the sibling species Trichogramma minutum Riley and Trichogramma platneri Nagarkatti. Biol Control. 2004;30:90-4.

Chang SC, Hu NT, Hsin CY, Sun CN. Characterization of differences between Two Trichogramma wasps by molecular markers. Biol Control. 2001;21(2):75-8.

Ciociola AI Jr, Querıno RB, Zucchı RA, Stouthamer R. Molecular tool for identification of closely related species A molecular key to the common species of Trichogramma of Trichogramma: T. rojasi Nagaraja and T. lasallei Pinto. Neotrop Entomol. 2001b;30(4):575-8.

Dang XL, Wen SY, He XF, Pang XF. M-PCR: a powerful method for rapid molecular identification of Trichogramma wasps. Insect Science. 2005;12:77-85.

Sappal NP, Jeng RS, Hubbes M, Liu F. Restriction fragment length polymorphisms in polymerase chain reaction amplified ribosomal DNAs of three Trichogramma species. Genome 1995;38:419-25.

Stouthamer R, Gai Y, Koopmanschap AB, Pinto JD, Platner GR. ITS-2 sequences do not differ for the closely related species Trichogramma minutum and T. platneri. Entomol Exp Appl. 2000a;95(1):105-11.

Stouthamer R, Jochemsen P, Platner GR, Pinto JD. Crossing incompatibility between Trichogramma minutum and T. platneri (Hymenoptera: Trichogrammatidae): implications for application in biological control. Environ Entomol. 2000b;29:832-7.

Truett GE, Heeger P, Mynatt, RL, Truett AA, Walker JA, Warman ML. Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT). BioTechniques. 2000;29(1):52-4.

Hebert PDN, Cyminska A, Ball SL, deWaard JR. Biological identifications through DNA barcodes. Proc R Soc Lond B Biol Sci . 2003;270:313-21.

Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. DNA primers for amplification of mitochondrial cytochrome C oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol. 1994;3:294-9.

Werren JH, Windsor DM. Wolbachia infection frequencies in insects: evidence of a global equilibrium. Proc R Soc Lond B Biol Sci. 2000;1277-85.

Zhou W, Rousset F, O’Neill S. Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences. Proc R Soc Lond B Biol Sci. 1998;265:509-15.

Razinger J, Vasileiadis VP, Giraud M, Dijk W, Modic Š, Sattin M, Urek G: On-farm evaluation of inundative biological control of Ostrinia nubilalis (Lepidoptera: Crambidae) by Trichogramma brassicae (Hymenoptera: Trichogrammatidae) in three European maize-producing regions. Pest Manag Sci 2016;72:246-54.

DeBach P, Hagen KS. Manipulation entomophagous species. In: DeBach P, editor. Biological control of insect pests and weeds. London: Chapman and Hall; 1964. p. 429-58.

Van Driesche R, Bellows TS Jr. Biological control. New York: Chapman and Hall; 1996. 539 p.

Colinet H, Boivin G. Insect parasitoids cold storage: A comprehensive review of factors of variability and consequences. Biol Control. 2011;58:83-95.

Burbutis PP, Curl, GD, Davis CP. Host searching behavior by Trichogramma nubilaleon corn. Environ Entomol. 1977;6(3):400-2.

Kot J. Analysis of factors affecting the phytophage reduction by Trichogramma Westw. species. Pol Ecol Stud. 1979;5:5-59.

Greenberg S. Evolution techniques for Trichogramma quality. In: Bigler F, editor. Proceedings of the 5th Workshop of the IOBC Global Working Group “Quality Control of Mass Reared Arthropods”; 1991 Mar 25-28; Wageningen, Netherland. Zurich: Swiss Federal Research Station for Agronomy; 1991. p. 138-45.

Kazmer DJ, Luck RF. Field test of the size-fitness in the egg parasitoid Trichogramma pretiosum. Ecology. 1995;76(2):412-25.

Cerutti F, Bigler F. Quality assessment of Trichogramma brassicae in the laboratory. Entomol Exp App. 1995;75(1):19-26.

Drost YC, Qiu YT, Posthuma-Doodeman CJAM, Van Lenteren JC. Comparison of searching starategies of five parasitoid species of Bemisia argentifolli Bellow and Perring (Hom., Aleyrodidae). J Appl Entomol. 2000;124:105-12.

Suverkropp BP, Bigler F, Van Lenteren JC. Temperature influences walking speed and walking activity of Trichogramma brassicae (Hym., Trichogrammatidae). J Appl Entomol. 2001;125:303-7.

De Almeida RP. Trichogramma and its relationship with Wolbachia: Identification of Trichogramma species, phylogeny, transfer and costs of Wolbachia symbionts dissertation]. Wageningen]: Wageningen University; 2004. 150 p.

Nazeri M, Ashouri A, Hosseini M. Can Wolbachia infection improve qualitative characteristics of Trichogramma brassicae reared on cold stored eggs of the host? Int J Pest Manag. 2015;61(3):243-9.

Stouthamer R, Luck RF. Influence of microbe-associated parthenogenesis on the fecundity of Trichogramma deion and T. pretiosum. Entomol Exp Appl. 1993;67:183-92.

Wang Z, Smith SM. Phenotypic differences between thelytokous and arrhenotokous Trichogramma minutum from Zeiraphera canadensis. Entomol Exp Appl. 1996;78:315-23.

Silva IMMS, Van Meer MMM, Roskam MM, Hoogenboom A, Gort G, Stouthamer R. Biological control potential of Wolbachia-infected versus uninfected wasps: laboratory and greenhouse evaluation of Trichogramma cordubensis and T. deion Strains. Biocontrol Sci Technol. 2000;10:223-38.

Hohmann CL, Luck RF, Stouthamer R. Effect of Wolbachia on the survival and reproduction of Trichogramma kaykai Pinto & Stouthamer (Hymenoptera: Trichogrammatidae). Neotrop Entomol. 2001;30:607-12.

Huigens ME, Stouthamer R. Parthenogenesis associated with Wolbachia. In: Bourtzis K, Miller TA, editors. Insect symbiosis. Boca Raton: CRC press; 2003. p. 247-66.

Rechcigl JE, Rechcigl NA. Biological and biotechnological control of insect pests. Boca Raton: CRC Press; 2000. 375 p.

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Published

2018-08-20

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Ivezić A, Rugman-Jones P, Stouthamer R, Ignjatović-Ćupina A. Molecular identification of Trichogramma egg parasitoids of Ostrinia nubilalis in northeastern Serbia. Arch Biol Sci [Internet]. 2018Aug.20 [cited 2024Apr.19];70(3):425-32. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/2365

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Vol. 70 No. 3 (2018)

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