Analysis of putative sclerotia maturation-related gene expression in Rhizoctonia solani AG1-IA

Authors

  • Bo Liu 1. Institute of Plant Immunology, Shenyang Agricultural University, Shenyang, Liaoning; 2. College of Life Sciences, Yan’an University, Yan’an, Shaanxi http://orcid.org/0000-0002-8342-8613
  • Zhoujie Ma Institute of Plant Immunology, Shenyang Agricultural University, Shenyang, Liaoning
  • Xiaotong Gai Institute of Plant Immunology, Shenyang Agricultural University, Shenyang, Liaoning
  • Yanqiu Sun Institute of Plant Immunology, Shenyang Agricultural University, Shenyang, Liaoning
  • Yanfeng Wang College of Life Sciences, Yan’an University, Yan’an, Shaanxi
  • Shidao He Institute of Plant Immunology, Shenyang Agricultural University, Shenyang, Liaoning
  • Zenggui Gao Institute of Plant Immunology, Shenyang Agricultural University, Shenyang, Liaoning

Keywords:

Rhizoctonia solani AG1-IA, expressed sequence tags, qRT-PCR, sclerotial formation, sheath blight of maize, cDNA library

Abstract

Paper description:

  • A cDNA library of Rhizoctonia solani AG1-IA, a major soil-borne fungal pathogen of maize, was constructed and qRT-PCR analysis was performed to determine the expression of twelve R. solani AG1-IA genes during three stages of sclerotial formation (mycelium, initiation of sclerotium and maturation of sclerotium).
  • The results of our study provide new findings related to the methods of prevention and control of disease caused by R. solani AG1-IA.


Abstract：Rhizoctonia solani AG1-IA (R. solani AG1-IA) is a major soil-borne fungal pathogen of maize that causes significant yield losses in all maize-growing regions worldwide. The sclerotium produced by R. solani AG1-IA can overwinter in grass roots or diseased plants and infect crops the following year. The molecular mechanism underlying sclerotium formation in R. solani is poorly understood. In this study, we constructed the cDNA library of the R. solani AG1-IA pathogenic strain WF-9, from which 329 high-quality expressed sequence tags (ESTs) were obtained. Of the 250 clustered unigenes, 12 genes were selected for further expression analysis during the three stages of sclerotial growth (mycelium, initiation of sclerotium, and maturation of sclerotium). The results of expression analysis support the previously suggested roles of chitin synthase D and exo-beta-1,3-glucanase in facilitating sclerotial growth through preservation of water content and energy. In addition, cytochrome P450, NADPH oxidase, catalase (CAT), acyl-CoA oxidase 1 (ACOX1), mitogen-activated protein kinase (MAPK), mitogen-activated protein kinase HOG1 (HOG 1), and the G-protein α subunit play important roles in balancing reactive oxygen species (ROS) levels during sclerotial development. The findings of this study can help understand the molecular mechanism of sclerotial development further.

https://doi.org/10.2298/ABS180106026L

Received: January 6, 2018; Revised: May 1, 2018; Accepted: May 4, 2018; Published online: Jun 14, 2018

How to cite this article: Liu B, Ma Z, Gai X, Sun Y, Wang Y, He S, Gao Z. Analysis of putative sclerotia maturation-related gene expression in Rhizoctonia solani AG1-IA. Arch Biol Sci. 2018;70(4):647-53.

Downloads

Download data is not yet available.

Author Biographies

Bo Liu, 1. Institute of Plant Immunology, Shenyang Agricultural University, Shenyang, Liaoning; 2. College of Life Sciences, Yan’an University, Yan’an, Shaanxi


Zhoujie Ma, Institute of Plant Immunology, Shenyang Agricultural University, Shenyang, Liaoning


Yanqiu Sun, Institute of Plant Immunology, Shenyang Agricultural University, Shenyang, Liaoning


References

Zheng A, Lin R, Zhang D, Qin P, Xu L, Ai P, Ding L, Wang Y, Chen Y, Liu Y. The evolution and pathogenic mechanisms of the rice sheath blight pathogen. Nat Commun. 2013;4:1424.

Ogoshi A. Ecology and pathogenicity of anastomosis and intraspecific groups of Rhizoctonia solani Kuhn. Annu Rev Phytopathol. 1987;25(1):125-43.

Sattari A, Fakheri B, Noroozi M. Breeding for resistance to sheath blight in rice. Intl J Farm Alli Sci. 2014;3(9):970-79.

Gautam H, Bhardwaj M, Kumar R. Climate change and its impact on plant diseases. Curr Sci. 2013;105(12):1685-91.

Renfro B, Ullstrup A. A comparison of maize diseases in temperate and in tropical environments. PANS. 1976;22(4):491-8.

Prasanna B, Pixley K, Warburton ML, Xie CX. Molecular marker-assisted breeding options for maize improvement in Asia. Mol Breed. 2010;26(2):339-56.

Bruce A, McDonald, Celeste L. Pathogen population genetics, evolutionary potential, and durable resistance. Annu Rev Phytopathol. 2002;40(1):349-79.

Moni ZR, Ali MA, Alam MS, Rahman MA, Bhuiyan MR, Mian MS, Iftekharuddaula KM, Latif MA, Khan MA. Morphological and genetical variability among Rhizoctonia solani isolates causing sheath blight disease of rice. Rice Sci. 2016;23(1):42-50.

Townsend BB, Willetts HJ. The development of sclerotia in certain fungi. Trans Br Mycol Soc. 1954;37(3):213-21.

Kwon YS, Kim SG, Chung WS, Bae H, Jeong SW, Shin SC, Jeong MJ, Park SC, Kwak YS, Bae DW. Proteomic analysis of Rhizoctonia solani AG-1 sclerotia maturation. Fungal Biol. 2014;118(5-6):433-43.

Charoensopharat K, Aukkanit N, Thanonkeo S, Saksirirat W, Thanonkeo P, Akiyama K. Targeted disruption of a G protein α subunit gene results in reduced growth and pathogenicity in Rhizoctonia solani. World J Microbiol Biotechnol. 2008;24(3):345-51.

Peraza L, Hansberg W. Neurospora crassa catalases, singlet oxygen and cell differentiation. Biol Chem. 2002;383(3-4):569-75.

Kim HJ, Chen C, Kabbage M, Dickman MB. Identification and characterization of Sclerotinia sclerotiorum NADPH oxidases. Appl Environ Microbiol. 2011;77(21):7721-9.

Soares MB, Bonaldo MF, Jelene P, Su L, Lawton L, Efstratiadis A. Construction and characterization of a normalized cDNA library. Proc Nati Acad Sci. 1994;91(20):9228-32.

Gasic K, Hernandez A, Korban SS. RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction. Plant Mol Biol Rep. 2004;22(4):437-8.

Wiemann S, Mehrle A, Bechtel S, Wellenreuther R, Pepperkok R, Poustka A, Consortium GC. cDNAs for functional genomics and proteomics: the German Consortium. C R Biol. 2003;326(10):1003-9.

Zhu Y, Machleder E, Chenchik A, Li R, Siebert P. Reverse transcriptase template switching: A SMART™ approach for full-length cDNA library construction. Biotechniques. 2001;30(4):892-7.

Gunning P, Ponte P, Okayama H, Engel J, Blau H, Kedes L. Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed. Mol Cell Biol. 1983;3(5):787-95.

Seki M, Narusaka M, Ishida J, Nanjo T, Fujita M, Oono Y, Kamiya A, Nakajima M, Enju A, Sakurai T. Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J. 2002;31(3):279-92.

Rioux R, Manmathan H, Singh P, De los Reyes B, Jia Y, Tavantzis S. Comparative analysis of putative pathogenesis-related gene expression in two Rhizoctonia solani pathosystems. Curr Genet. 2011;57(6):391-408.

Zhulidov P, Bogdanova E, Shcheglov A, Shagina I, Wagner L, Khazpekov G, Kozhemyako V, Lukyanov S, Shagin D. A method for the preparation of normalized cDNA libraries enriched with full-length sequences. Russ J Bioorganic Chem. 2005;31(2):170-7.

Huang X,Madan A. CAP3: A DNA Sequence Assembly Program. Genome Res. 1999;9(9):868-77.

Martin DM, Bohlmann J. Identification of Vitis vinifera (−)-α-terpineol synthase by in silico screening of full-length cDNA ESTs and functional characterization of recombinant terpene synthase. Phytochem. 2004;65(9):1223-9.

Tatusov RL, Galperin MY, Natale DA, Koonin EV. The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res. 2000;28(1):33-6.

Tatusov RL, Fedorova ND, Jackson JD, Jacobs AR, Kiryutin B, Koonin EV, Krylov DM, Mazumder R, Mekhedov SL, Nikolskaya AN. The COG database: an updated version includes eukaryotes. BMC Bioinformatics. 2003;4(1):41.

Georgiou CD, Patsoukis N, Papapostolou I, Zervoudakis G. Sclerotial metamorphosis in filamentous fungi is induced by oxidative stress. Integr Comp Biol. 2006;46(6):691-712.

Morais S, Knoll-Gellida A, André M, Barthe C, Babin PJ. Conserved expression of alternative splicing variants of peroxisomal acyl-CoA oxidase 1 in vertebrates and developmental and nutritional regulation in fish. Physiol Genomics. 2007;28(3):239.

Aguirre J, Rios-Momberg M, Hewitt D, Hansberg W. Reactive oxygen species and development in microbial eukaryotes. Trends Microbiol. 2005;13(3):111-8.

Willetts HJ, Bullock S. Developmental biology of sclerotia. Mycol Res. 1992;96(10):801-16.

Downloads

Published

2018-12-04

How to Cite

1.
Liu B, Ma Z, Gai X, Sun Y, Wang Y, He S, Gao Z. Analysis of putative sclerotia maturation-related gene expression in Rhizoctonia solani AG1-IA. Arch Biol Sci [Internet]. 2018Dec.4 [cited 2024Dec.22];70(4):647-53. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/2526

Issue

Vol. 70 No. 4 (2018)

Section

Articles

License

Authors grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.

Most read articles by the same author(s)