Antidiabetic, hematoprotective and nephroprotective effects of the aqueous extract of Falcaria vulgaris in diabetic male mice

Authors

  • Mohammad Mahdi Zangeneh 1. Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah; 2. Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam
  • Akram Zangeneh 1. Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah; 2. Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam
  • Reza Tahvilian Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah
  • Rohallah Moradi Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam

Keywords:

Falcaria vulgaris, aqueous extract, hypoglycemic activity, nephroprotective activity, streptozotocin

Abstract

Paper description:

  • The antidiabetic effects of Falcaria vulgaris aqueous extract collected in Iran has not been evaluated.
  • We examined the hematological and renal changes of this extract in streptozotocin-induced diabetic mice by stereology. Our findings show that the aqueous extract of F. vulgaris produces favorable antidiabetic, hematoprotective and nephroprotective effects.
  • The obtained results recommend examination of the extract in clinical studies on advancing medical therapy of diabetes mellitus.

Abstract: Falcaria vulgaris has been used in medicine as an antimicrobial agent. The aims of this study were to evaluate the hypoglycemic and nephroprotective activities of the aqueous extract of F. vulgaris in diabetic mice. Diabetes was experimentally induced by intraperitoneal injection of streptozotocin (STZ). The mice were divided randomly into 6 groups as follows: nondiabetic, untreated diabetic, a group that received orally 0.5 mg/kg glibenclamide, and three groups that were given orally 200, 600 and 1800 µg/kg of F. vulgaris, respectively, for 20 days. On the 20th day, the mice were dissected, and blood and kidney samples were collected for analysis of hematological, biochemical and stereological parameters. F. vulgaris at all doses, particularly 1800 µg/kg, significantly (p≤0.05) reduced the levels of urea, creatinine, WBC, eosinophils, basophiles, platelet and increased RBC, Hb, PCV, MCV, MCH, MCHC, lymphocytes and monocytes that were raised after diabetes induction, compared to the untreated diabetic group. Multiple doses of F. vulgaris, particularly1800 µg/kg, significantly (p≤0.05) decreased the total volumes of the kidney cortex, medulla, vessels and renal tubules, as well as the lengths of the vessels and renal tubules, compared to the untreated diabetic group. Also, F. vulgaris at all doses significantly prevented glomerular hypertrophy and reduction of glomeruli numbers in comparison with the untreated diabetic group. In conclusion, F. vulgaris possesses antidiabetic, hematoprotective and nephroprotective properties and can improve renal structural and blood biomarkers in STZ-induced nephrotoxicity in mice.

https://doi.org/10.2298/ABS180222027Z

Received: February 22, 2018; Revised: June 11, 2018; Accepted: June 11, 2018; Published online: June 15, 2018

How to cite this article: Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R. Antidiabetic, hematoprotective and nephroprotective effects of the aqueous extract of Falcaria vulgaris in diabetic male mice. Arch Biol Sci. 2018;70(4):655-64.

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References

Le Devehat C, Khodabandehlou T, Vimeux M. Impaired hemorheological properties in diabetic patients with lower limb arterial ischaemia. Clin Hemorheol Microcirc. 2001;25(2):43-8.

Musabayane CT. The effects of medicinal plants on renal function and blood pressure in diabetes mellitus. Cardiovasc J Afr. 2012;23(8):462-8.

Hostetter TH, Troy JL, Brenner BM. Glomerular hemodynamic in experimental diabetes. Kidney Int. 1981;19(3):410-5.

Mogensen CE, Anderson MJF. Increased kidney size and glomerular filtration rate in untreated juvenile diabetes: normalization by insulin-treatment. Diabetologia. 1975;11(3):221-4.

Christiansen JS, Gammelgaard J, Tronier B, Svendsen PA, Parving HH. Kidney function and size in diabetes before and during initial insulin treatment. Kidney Int. 1982;21(5):683-8.

Wiseman MJ, Saunders AJ, Keen H, Viberti GC. Effect of blood glucose control on increased glomerular filtration rate and kidney size in insulin dependent diabetes. N Engl J Med. 1985;312(10):617-21.

Barnes A, Willars E. “Diabetes” in Clinical Hemorheology. In: Chien S, Dormandy J, Ernst E, A Matrai A, editors. Dordrecht: Martinus Nijhoff; 1987. p. 275-309.

Rahimi R, Nikfar S, Larijani B, Abdollahi M. A review on the antioxidants in the management of diabetes and its complications. Biomed Pharmacother 2005;59(7):365-73.

Abdel-Barry JA, Abdel-Hassan IA, Al-Hakiem MHH. Hypoglycaemic and antihyperglycaemic effects of Trigonella foenum-graecum leaf in normal and alloxan induced diabetic rats. J Ethnopharmacol. 1997;58(3):149-55.

Pushparaj P, Tan CH, Tan BKH. Effects of Averrhoa Bilimbi leaf extract on blood glucose and lipids in streptozotocin-diabetic rats. J Ethnopharmacol. 2000;72(1):69-76.

WHO Expert Committee on Diabetes mellitus. Technical Report Series 646, Second Report. World Health Organization Geneva, 1981.

Najafi F, Tahvilian R, Zangeneh MM, Zangeneh A, Moradi R. Screening of essential oil of Allium sativum for antibacterial effects against Bacillus subtilis. Int J Rec Sci Res. 2016;7(11):14172-6.

Tahvilian R, Moradi R, Hajialiani M, Zangeneh MM, Zangeneh A, Yazdani H, Zhaleh H. Evaluation of Antibacterial properties of essential oil of Pistacia khinjuk. Int J Res Pharm Sci. 2017;7(1):1-6.

Poorshamohammad C, Souri N, Amini Z, Kosari F, Jamshidpour R, Zangeneh MM, Zangeneh A. Cucurbita moschata: A plant with antibacterial properties. Int J Curr Med Pharm Res. 2017;3(2):1356-9.

Faramarzi E, Zangeneh MM, Zangeneh A, Moradi R. Effect of Cinnamomum zelanicumon oil on hyponeophagia anxiety test in Balb C male mice. Onl J Vet Res. 2017;21(2):77-80.

Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zamgeneh A, Tahvilin R, Moradi R. Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol. 2017;26 (2):455-63.

Najafi F, Goodarzi N, Zangeneh MM, Zangeneh A, Hagh-Nazari L. Antidiabtic and Hepatoprotective Effects of Bitter Fraction of Stevia rebaudiana Alcoholic Extract on Streptozotocin-Induced Diabetic Male Mice. J Rafsanjan Univ Med Sci. 2017;16(6):493-504.

Shojaii A, Hashem-Dabaghian F, Goushegir A, Abdollahi-Fard M. Antidiabetic Plants of Iran. Acta Medica Iranica. 2011;49(10):637-42.

Ahvazi M, Khalighi-Sigaroodi F, Charkhchiyan MM, Mojab F, Mozaffarian VA, Zakeri H. Introduction of medicinal plants species with the most traditional usage in Alamut region. Iran J Pharm Res. 2012;11(1):185-94.

Jaberian H, Piri K, Nazari J. Phytochemical composition and in vitro antimicrobial and antioxidant activities of some medicinal plants. Food Chem. 2013;136(1):237-44.

Shakibaie D, Pasharavesh L, Khoshboo S, Kaboodi B. The Effect of the “Falcaria Vulgaris” on Deep Skin Wound Remodeling Time and Skin Tension Power in Rats. J Kermanshah Univ Med Sci. 2007;10(3):187-94.

Jivad N, Bahmani M. A review of important medicinal plants native to Iran effective on recovery from peptic ulcer. Der Pharm Lett. 2016;8(2):347-52.

Braendgaard H, Gundersen HJ. The impact of recent stereological advances on quantitative studies of the nervous system. J Neurosci Methods. 1986;18(1-2):39-78.

Gundersen HJ, Bendtsen TF, Korbo L, Marcussen N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A, West MJ. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS. 1992;96(5):379-94.

Nyengaard JR. Stereologic methods and their application in kidney research. J Am Soc Nephrol. 1999;10(5):1100-23.

Mandarim-de-Lacerda CA. Stereological tools in biomedical research. An Acad Bras Cienc. 2003;75(4):469-86.

Ahmed D, Kumar V, Verma A, Gupta PS, Kumar H, Dhingra V, Mishra V, Sharma M. Antidiabetic, renal/hepatic/pancreas/cardiac protective and antioxidant potential of methanol/dichloromethane extract of Albizzia Lebbeck Benth. stem bark (ALEx) on streptozotocin induced diabetic rats. Complementary altern med. 2014;14:243-60.

Tahvilian R, Moradi R, Zhaleh H, Zangeneh MM, Zangeneh A, Yazdani H, Hajialiani M. Chemical composition and screening of antibacterial activity of essential oil of Pistacia khinjuk against two selected pathogenic bacteria. Ann Trop Med Public Health. 2017;10(5):1159-64.

Foroughi A, Zangeneh MM, Zangeneh A, Kazemi N. A survey on antibacterial activities of Allium eriophyllum alcoholic extract: an ethnomedicinal plant. Iran J Publ Health. 2016;45(2):32.

Moradi R, Hajialiani M, Zangeneh MM, Zangeneh A, Faizi S, Zoalfaghari M, Marabi A. Study a plant extract as an antibacterial agent. Int J Curr Med Pharm Res. 2017;3(2):1360-2.

Ghashghaii A, Hashemnia M, Nikousefat Z, Zangeneh MM, Zangeneh A. Wound healing potential of methanolic extract of Scrophularia striata in rats. Pharm Sci. 2017;23(4):256-63.

Zangeneh MM, Najafi F, Tahvilian R, Salmani S, Haghnazari L, Zangeneh A, Moradi R. Ethnomedicinal plants: in vitro antibacterial effects of ethanolic extract of Stevia rebaudiana. Int J Ayu Pharm Chem. 2017;6(1):251-9.

Miles RE, Davy P. On the choice of quadrats in stereology. J Microsc. 1977;110(1): 27-44.

Cruz-Orive LM. On the estimation of particle number. J Microsc. 1980;120(1): 15-27.

Breyer MD, Bottinger E, Brosius FC, Coffman TM, Harris RC, Heilig CW, Sharma K. Mouse models of diabetic nephropathy. J Am Soc Nephrol. 2005;16(1);27-45.

Brosius FC, Alpers CE, Bottinger EP, Breyer MD, Coffman TM, Gurley SB, Harris RC, Kakoki M, Kretzler M, Leiter EH, Levi M, McIndoe RA, Sharma K, Smithies O, Susztak K, Takahashi N, Takahashi T. Mouse models of diabetic nephropathy. J Am Soc Nephrol. 2009;20(12):2503-12.

Tesch GH, Allen TJ. Rodent models of streptozotocininduced diabetic nephropathy. Nephrology (Carlton). 2007;1(3):261-6.

Lenzen S. The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia. 2008;51(2):216-26.

Weiss RB. Streptozocin: a review of its pharmacology, efficacy, and toxicity. Cancer Treat Rep. 1982;66(3):427-38.

Rerup CC. Drugs producing diabetes through damage of the insulin secreting cells. Pharmacol Rev. 1970;22(4):485-518.

Tay YC, Wang Y, Kairaitis L, Rangan GK, Zhang C, Harris DCH. Can murine diabetic nephropathy be separated from superimposed acute renal failure? Kidney Int. 2005;68(1):391-38.

Kraynak AR, Storer RD, Jensen RD, Kloss MW, Soper KA, Clair JH, DeLuca JG, Nichols WW, Eydelloth RS. Extent and persistence of streptozotocin-induced DNA damage and cell proliferation in rat kidney as determined by in vivo alkaline elution and BrdUrd labeling assays. Toxicol Appl Pharmacol. 1995;135(2):279-86.

Palm F, Ortster H, Hansell P, Liss P, Carlsson PO. Differentiating between effects of streptozotocin per se and subsequent hyperglycemia on renal function and metabolism in the streptozotocin diabetic rat model. Diabetes Metab Res Rev. 2004;20(6): 452-9.

Rafiey Z, Jalili F, Sohrabi M, Salahshoor M, Jalili C. Effects of hydro- alcoholic extract of Falcaria vulgaris on pancreas tissue in streptozotocin-induced diabetic rats. Iranian J Endocrinol Metab. 2017;9(2):91-9.

Saba AB, Oyagbemi AA, Azeez OI. Amelioration of carbon tetrachloride-induced hepatotoxicity and haemotoxicity by aqueous leaf extract of Cnidoscolus aconitifolius in rats. Nig J Physiol Sci. 2010;25(2):139-47.

Huff GR, Huff WE, Balog JM, Rath NC, Anthony NB, Nestor KE. Stress response differences and disease susceptibility reflected by heterophil to lymphocyte ratio in turkeys selected for increased body weight. Poult Sci. 2005;84(5):709-17.

Larson CT, Gross WB, Davis JW. Social stress and resistance of chicken and swine to Staphylococcus aureus challenge infections. Can J Comp Med. 1985;49(2):208-10.

Halliwell B, Gutteridge J. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J. 1984;219(1):1-14.

Masuda Y. Learning toxicology from carbon tetrachloride-induced hepatotoxicity. Yakugaku Zasshi. 2006;126(10):885-99.

Rood AS, McGavran PD, Aanenson JW, Till JE. Stochastic estimates of exposure and cancer risk from carbon tetrachloride released to the air from the rocky flats plant. Risk Anal. 2001;21(4):675-95.

Slater TF. Free radical mechanisms in tissue injury. Biochem J. 1984;222(1):1-15.

Mishra S, Ranjan-Pani S, Sahoo S. Anti-nephrotoxic activity of some medicinal plants from tribal rich pockets of Odisha. Pharmacogn Res. 2014;6(3):210-7.

Khanahmadi M, Shahrezaei F. Review and identify the chemical constituents of volatile oils of Falcaria vulgaris Bernh. J Med Plants. 2008;6(3):52-7.

Shafaghat A. Volatile oil constituents and antibacterial activity of different parts of Falcaria vulgaris Bernh. growing wild in two localities from Iran. Nat Prod Res. 2011;25(4):368-73.

Zangeneh MM, Goodarzi N, Zangeneh A, Najafi F, Tahvilian R. Hypoglycemic and nephroprotective effects of aqueous extract of Stevia rebaudiana (sweet fraction) in streptozotocin-induced diabetic mice. J Ardabil Univ Med Sci. 2018;17(4):437-46.

Kishore L, Kaur N, Randhir S. Nephroprotective effect of Paeonia emodi via inhibition of advanced glycation end products and oxidative stress in streptozotocin–nicotinamide induced diabetic nephropathy. J Food Drug Anal. 2017;25(3):576-88.

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Published

2018-12-04

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1.
Zangeneh MM, Zangeneh A, Tahvilian R, Moradi R. Antidiabetic, hematoprotective and nephroprotective effects of the aqueous extract of Falcaria vulgaris in diabetic male mice. Arch Biol Sci [Internet]. 2018Dec.4 [cited 2024Oct.30];70(4):655-64. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/2686

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Vol. 70 No. 4 (2018)

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