In vivo and in silico investigations of the toxicological and analgesic properties of unprocessed Aloe vera gel in experimental rat models
Keywords:
Aloe vera, nociception, molecular docking, Aloe-emodin, hepatitis B viral proteinAbstract
Paper description:
- The Aloe vera plant gel is one of the most commonly used ethno-pharmacological herbal resources containing different medicinal activities.
- We examined the toxicity of the plant gel when consumed orally for an extended time in an experimental rat model and by in silico approaches.
- The present work provides novel information regarding the safety of A. vera gel. Its analgesic property is also examined.
Abstract: Aloe vera is a commonly used plant in both food and medicine industry. The potential toxicological side-effects of prolonged intake of Aloe extract have not been evaluated in detail. This work presents an in-depth toxicological study of the crude unprocessed A. vera gel in experimental rats. Acute and sub-chronic toxicity was evaluated in a 1 to 28-day long feeding schedule of the aqueous homogenized gel material. Hemoglobin, total protein, high density lipoprotein (HDL), low density lipoprotein (LDL), cholesterol, triglyceride, serum creatinine, serum alanine transaminase (SGPT), aspartate transaminase (SGOT) and alkaline phosphatase were examined and kidney and liver histology was performed. In the acute toxicity test, the behavioral aspects were also considered. A molecular docking assay was performed to investigate the binding affinities of pure A. vera compounds with liver and kidney toxicological marker enzymes, in order to assess the probable mode of action of selected Aloe constituents. Solubility factors for the active constituents were also studied to determine their possible miscibility with body fluids. The results from in vivo tests provided no major toxicological indications. Crude Aloe gel consumption up to 4 g/kg body weight (b.w.) showed no toxicological side effects. From the structural standpoint, Aloe-based bioactive molecules, such as Aloe-emodin, acetophenone, β-sitosterol, cholestenol and squalene showed promising binding affinity to qualify as alternative and complementary medicines. The synergistic roles of all A. vera constituents remain to be validated in human disease models.
https://doi.org/10.2298/ABS180524036P
Received: May 24, 2018; Revised: July 11, 2018; Accepted: July 13, 2018; Published online: August 9, 2018
How to cite this article: Paul S, Chakraborty AK, Modak D, Sen A, Bhattacharjee S. In vivo and in silico investigations of the toxicological and analgesic properties of unprocessed Aloe vera gel in experimental rat models. Arch Biol Sci. 2018;70(4):727-35.
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References
Atiba A, Nishimura M, Kakinuma S. Aloe vera oral administration accelerates acute radiation-delayed wound healing by stimulating transforming growth factor-2 and fibroblast growth factor production. Am J Surgery. 2011;201(6):809-18.
Foster M, Hunter D, Samman S. Evaluation of the nutritional and metabolic effects of Aloe vera. In: Benzie IFF, Wachtel-Galor S, editors. Herbal Medicine: Biomolecular and Clinical Aspects. 2nd edition. Boca Raton (FL): CRC Press/Taylor & Francis; 2011. Chapter 3..
Reynolds T, Dweck AC. Aloe vera leaf gel: a review update. J Ethnopharmacol. 1999;68(1):3-37.
Paul S, Dutta T, Chaudhuri TK, Bhattacharjee S. Curative and protective properties of crude gel of Aloe vera from sub-Himalayan West Bengal in chronic and acute inflammatory rat models. Ind J Trad Knowl. 2017;16(1):121-7.
Vogler BK, Ernst E. Aloe vera: a systematic review of its clinical effectiveness. Br J Gen Pract. 1999;49(447):823-8.
Nejatzadeh-Barandozi F. Antibacterial activities and antioxidant capacity of Aloe vera. Org Med Chem Letters. 2013;3(1):1-5.
Guo X, Mei N. Aloe vera: A review of toxicity and adverse clinical effects. J Environ Sci Health, Part C. 2016;34:77-96.
Gupta R, Flora SJS. Protective value of Aloe vera against some toxic effects of arsenic in rats. Phytother Res. 2005;1:23-8.
Anilakumar KR, Sudarshanakrishna KR, Chandramohan G, Ilaiyaraja N, Khanum F, Bawa AS. Effect of Aloe vera gel extract on antioxidant enzymes and azoxymethane-induced oxidative stress in rats.Ind J Exp Biol. 2010;48(8):837-42.
Arosio B. Aloe emodinquinine pretreatment reduces acute liver injury induced by carbon tetrachloride. Basic Clin Pharmacol Toxicol. 2010;87:229-33.
National Toxicology Program. NTP Toxicology and Carcinogenesis Studies of EMODIN (CAS NO. 518-82-1) Feed Studies in F344/N Rats and B6C3F1 Mice National Toxicology Program technical report series. Natl Toxicol Program Tech Rep Ser. 2001;493:1-278..
Saljooghianpour M, Javaran TA. Identification of phytochemical components of Aloe plantlets by gas chromatography-mass spectrometry. Afr J Biotech. 2013;12(49):6876-80.
Sharif HB, Mukhtar MD, Mustapha Y, Baba G, Lawal AO. Acute and subchronic toxicity profile of Euphorbia pulcherrima methanol extract on Wistar albino rats. Adv Pharm. 2015;2015:539646.
Yuet Ping K, Darah I, Chen Y, Sreeramanan S, Sasidharan S. Acute and subchronic toxicity study of Euphorbia hirta L. methanol extract in rats. BioMed Res Int. 2013;2013:182064.
Wheeler-Aceto H, Porreca F, Cowan A. The rat paw formalin test: comparison of noxious agents. Pain. 1990;40:229-38.
Wheeler-Aceto H, Cowan A. Standardization of the rat paw formalin test for the evaluation of analgesics. Psychopharmacology. 1991;104:35-44.
Hunskaar S, Hole K. The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain. 1987;30:103-14.
Trott O, Olson AJ. Auto Dock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J Comput Chem. 2010;31(2):455-61.
Tetko IV, Tanchuk VY. Application of associative neural networks for prediction of lipophilicity in ALOGPS 2.1 program. J Chem Info Comp Sci. 2002;42(5):1136-45.
Roopashree TS, Raman D, Rani RHS, Narendra C. Acute oral toxicity studies of antipsoriatic herbal mixture comprising of aqueous extracts of Calendula officinalis, Momordica charantia, Cassia tora and Azadirachta indica seed oil. Thai J Pharm Sci. 2009;33:74-83.
Raza M, Al-Shabanah OA, El-Hadiyah TM, Al-Majed AA. Effect of prolonged vigabatrin treatment on hematological and biochemical parameters in plasma, liver and kidney of Swiss albino mice. Scientia Pharmaceutica. 2002;70:135-45.
Brautbar N, Williams J. Industrial solvents and liver toxicity: risk assessment, risk factors and mechanisms. Int J Hygiene Eenviron Health. 2002;205:479-91.
Giboney PT. Mildly elevated liver transaminase levels in the asymptomatic patient. Am Fam Physician. 2005;71:1105-10.
Kew MC. Hepatitis B virus x protein in the pathogenesis of hepatitis B virus-induced hepatocellular carcinoma. J Gastroenterol Hepatol. 2011;26:144-52.
Abdalla SI, Sanderson IR, Fitzgerald RC. Effect of inflammation on cyclooxygenase (COX) -2 expression in benign and malignant oesophageal cells. Carcinogenesis. 2005;26:1627-33.
Paul S, Dutta S, Chaudhuri TK, Bhattacharjee S. Anti-inflammatory and protective properties of Aloe vera leaf crude gel in carrageenan induced acute inflammatory rat models. Int J Pharm Pharm Sci. 2014;6(9):368-71.
Guha P, Paul S, Das A, Halder B, Bhattacharjee S, Chaudhuri TK. Analyses of human and rat clinical parameters in rheumatoid arthritis raise the possibility of use of crude Aloe vera gel in disease amelioration. Immunome Res. 2014;10:1.
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