Examination of RAD51 gene G135C polymorphism in gastric cancer patients in northeastern Anatolia

Authors

  • Ilhami Gok Department of Bioengineering, Faculty of Engineering and Architecture, Kafkas University, Kars
  • Ozkan Ozden Department of Bioengineering, Faculty of Engineering and Architecture, Kafkas University, Kars

Keywords:

gastric cancer, RAD51 gene, genetic polymorphism, RFLP, DNA repair

Abstract

Paper description:

  • Gastric cancer is the third most common cause of cancer mortality, with cancer incidence varying among populations. RAD51 is a DNA repair gene associated with different cancer types in different populations.
  • G135C polymorphism of the RAD51 gene among gastric cancer patients and healthy individuals was genotyped to test the association between gene polymorphism and cancer prevalence in the northeastern Anatolian population for the first time.
  • Both genotypic and allelic frequencies of the polymorphism differed significantly between heathy and gastric cancer patients, implying an association between the C allele in the RAD51 gene and gastric cancer.

Abstract: Polymorphisms of DNA repair and genome integrity genes are associated with DNA repair capacity and elevated cancer risk. To establish an association between the pattern of polymorphism and the incidence of any type of cancer, studies across different populations are required. Polymorphic regions have been identified in the RAD51 repair gene in various cancer types; however, the influence of specific genetic variants on gastric cancer prevalence has not been empirically demonstrated. We conducted a case-control study with 76 gastric cancer patients and 78 healthy individuals from northeastern Anatolia to examine the association between polymorphism and gastric cancer. We genotyped the previously identified G135C polymorphism of RAD51 in all individuals and estimated the allele and genotype frequencies in the two groups. Our results indicated that the two groups differed both in allele and genotype frequencies. Additionally, a significant and elevated odd ratio (3.53) of gastric cancer for the C allele of RAD51 was observed. The genotypes GC and CC had also significant and high odd ratios (>3.75). Our results indicate that G135C polymorphism of the RAD51 gene was associated with an increased risk of gastric cancer in the examined population.

https://doi.org/10.2298/ABS181121002G

Received: November 21, 2018; Revised: December 25, 2018; Accepted: January 18, 2019; Published online: January 23, 2019

How to cite this article: Gok I, Ozden O. Examination of RAD51 gene G135C polymorphism in gastric cancer patients in northeastern Anatolia. Arch Biol Sci. 2019;71(2):209-13.

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Published

2019-06-04

How to Cite

1.
Gok I, Ozden O. Examination of RAD51 gene G135C polymorphism in gastric cancer patients in northeastern Anatolia. Arch Biol Sci [Internet]. 2019Jun.4 [cited 2024Dec.26];71(2):209-13. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/3793

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Vol. 71 No. 2 (2019)

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