Diversity of bacterial communities in the rhizosphere of the endangered plant, Paeonia jishanensis
Keywords:
Paeonia jishanensis, endangered plant, rhizosphere, microbial communities, metagenomic, next generation sequencingAbstract
Paper description:
- Paeonia jishanensis is a significant ancestral species and many cultivated tree peony species were bred from it. P. jishanensis is also a traditional Chinese herbal medicine. The species is facing extinction.
- This study describes for the first time the rhizosphere microbial communities of P. jishanensis with a different growth status.
- Our work suggests that the abundance of many potentially beneficial rhizospheric microbes have decreased with the decline of the plant.
- Inoculation of beneficial microbes could be a potential approach to protect those endangered plants.
Abstract: The microbial community in the rhizosphere is thought to provide plants with a second set of genomes, which plays a pivotal role in plant growth. In the present study, soil samples were collected from the rhizosphere of an endangered plant, Paeonia jishanensis. The plants were divided into three groups: well-growing plants, poor-growing plants and dead plants. Metagenomic DNA was isolated from rhizosphere soil samples of these plants and 16S rRNA genes were sequenced by the PacBio-RS II system. The results of taxonomic analysis showed that the dominant phyla were Proteobacteria, Acidobacteria, Planctomycetes, Bacteroidetes and Actinobacteria in all three sample types. Linear discriminate analysis Effect Size (LEfSe) showed that 5 species, Hirschia baltica, Arcobacter aquimarinus, Gimesia maris, Magnetococcus marinus and Pseudoxanthobactor soli, were significantly enriched in the rhizosphere of well-growing plants. Additionally, the results of PCA, MDS and clustering analysis indicated that the bacterial community in the rhizosphere of living P. jishanensis plants was similar. With the death of plants, the bacterial community changed considerably. These findings suggest that the abundance of many beneficial rhizospheric microbes declined with the death of P. jishanensis. This is a potential way to preserve endangered plants by inoculating declining species with beneficial microbes.
https://doi.org/10.2298/ABS190203036W
Received: February 3, 2019; Revised: May 29, 2019; Accepted: May 31, 2019; Published online: June 7, 2019
How to cite this article: Wang DS, Erihemu, Yang B. Diversity of bacterial communities in the rhizosphere of the endangered plant, Paeonia jishanensis. Arch Biol Sci. 2019;71(3):525-31.
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References
Hong D, Pan K, Zhou Z. Circumscription of Paeonia suffruticosa Andrews and identification of cultivated tree peonies. J Syst Evol. 2004;42(3):275-83.
Choi H, Seo H, Kim J, Um JY, Shin YC, Ko SG. Ethanol extract of Paeonia suffruticosa Andrews (PSE) induced AGS human gastric cancer cell apoptosis via fas-dependent apoptosis and MDM2-p53 pathways. J Biomed Sci. 2012;19(1):82-93.
Zhang F. The endangered causes of Paeonia suffruticosa var. spontanea, an endemic to China. Acta Ecologica Sinica. 2003;23(7):1436-41.
Saldajeno MGB, Ito M, Hyakumachi M. Interaction between the plant growth promoting fungus Phoma sp．GS8-2 and the arbuscular mycorrhizal fungus Glomus mosseae: Impact on biocontrol of soil-borne diseases, microbial population and plant growth. Australas Plant Path. 2012;41(3):271-81.
Bothe H, Turnau K, Regvar M. The potential role of arbuscular mycorrhizal fungi in protecting endangered plants and habitats. Mycorrhiza. 2010;20(7):445-57.
Mekonnen B, Yahya A, Alström S. Micro-organisms associated with endangered Cordeauxia edulis affect its growth and inhibit pathogens. Afr J Agr Res. 2010;5(24):3360-8.
Husna F, Tuheteru FD, Wigati E. Short Communication: Growth response and dependency of endangered nedun tree species (Pericopsis mooniana) affected by indigenous Arbuscular Mycorrhizal Fungi inoculation. Nusantara Bioscience. 2017;9(3):57-61.
Xu XX, Cheng FY, Xian HL, Peng LP. Genetic diversity and population structure of endangered endemic Paeonia jishanensis, in China and conservation implications. Biochem Syst Ecol. 2016;66(7):319-25.
Wu YN, Feng YL, Paré PW, Chen YL, Xu R, Wu S, Wang S, Zhao Q, Li H, Wang Y, Zhang J. Beneficial soil microbe promotes seed germination, plant growth and photosynthesis in herbal crop Codonopsis pilosula. Crop Pasture Sci. 2016;67(1):91-8.
Youssef NH, Elshahed MS. Diversity rankings among bacterial lineages in soil. ISME J. 2009;3(3): 305-13.
Harris J. Soil microbial communities and restoration ecology: facilitators or followers? Science, 2009;325(5940): 573-574.
Yanai RD, Fisk MC, Fahey TJ, Cleavitt NL, Park BB. Identifying roots of northern hardwood species: patterns with diameter and depth. Can J Forest Res. 2008;38(11):2862-9.
Ullah A, Akbar A, Luo Q, Khan AH, Manghwar H, Shaban M, Yang X. Microbiome diversity in cotton rhizosphere under normal and drought conditions. Microb Ecol. 2019;77(2):429-39.
Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R. QIIME allows analysis of highthroughput community sequencing data. Nat Methods. 2010;7(5):335-6.
Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Glöckner FO. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res. 2013;41(D1):590-6.
Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, Huttenhower C. Metagenomic biomarker discovery and explanation. Genome Biol. 2011;12(6):R60.
Kemp PF, Aller JY. Bacterial diversity in aquatic and other environments: what 16S rDNA libraries can tell us. FEMS Microbiol Ecol. 2004;47(2):161-77.
Ramette A. Multivariates analyses in microbial ecology. FEMS Microbiol Ecol. 2007;62(2):142-60.
Michaelis J, Diekmann M. Effects of soil types and bacteria inoculum on the cultivation and reintroduction success of rare plant species. Plant Ecol. 2018;219(4):441-53.
Finkel OM, Castrillo G, Paredes SH, Salas González I, Dangl JL. Understanding and exploiting plant beneficial microbes. Curr Opin Plant Biol. 2017;38(8):155-63.
Yan N, Marschner P, Cao W, Zuo C, Qin W. Influence of salinity and water content on soil microorganisms. J Soil Water Conserv. 2015;3(4):316-23.
Verbon EH, Liberman LM. Beneficial microbes affect endogenous mechanisms controlling root development. Trends Plant Sci. 2016;21(3):218-29.
Zhang H, Shangguan T. Comparison of element contents in Paeonia jishanensis of Jishan and Yongji, Shanxi, China. Chin J Appl Environ Biol. 2005;11(2):160-3.
Xue D, Huang X. Changes in soil microbial community structure with planting years and cultivars of tree peony (Paeonia suffruticosa). World J Microb Biot. 2014;30(2):389-97.
Islam MT , Hashidoko Y , Deora A, Ito T, Tahara S. Suppression of damping-off disease in host plants by the rhizoplane bacterium Lysobacter sp. strain SB-K88 is linked to plant colonization and antibiosis against soilborne Peronosporomycetes. Appl Environ Microb. 2005;71(7):3786-96.
Bazylinski DA, Williams TJ, Lefèvre CT, Berg RJ, Zhang CL, Bower SS. Magnetococcus marinus gen. nov. sp. nov. a marine, magnetotactic bacterium that represents a novel lineage (Magnetococcaceae fam. nov. Magnetococcales ord. nov.) at the base of the Alphaproteobacteria. Int J Syst Evol Micr. 2013;63(3):801-8.
Ferreira C, Soares AR, Lamosa P, Santos MA, da Costa MS. Comparison of the compatible solute pool of two slightly halophilic Planctomycetes species, Gimesia maris and Rubinisphaera brasiliensis. Extremophiles. 2016;20(6):1-10.
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