Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach

Authors

  • Milan B. Dragićević Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bul. despota Stefana 142, 11000 Belgrade http://orcid.org/0000-0002-9422-2952
  • Katarina B. Ćuković Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, P.O. Box 33, 11030 Belgrade http://orcid.org/0000-0001-5697-6457
  • Snežana R. Zdravković-Korać Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bul. despota Stefana 142, 11000 Belgrade http://orcid.org/0000-0002-8912-6886
  • Ana D. Simonović Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bul. despota Stefana 142, 11000 Belgrade http://orcid.org/0000-0003-4909-4493
  • Milica D. Bogdanović Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bul. despota Stefana 142, 11000 Belgrade http://orcid.org/0000-0001-8034-2606
  • Slađana I. Todorović Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bul. despota Stefana 142, 11000 Belgrade http://orcid.org/0000-0002-8233-9037

Keywords:

germination, glutamine synthetase, grain filling, knockout mutant, phenotype

Abstract

Abstract: Glutamine synthetase (E.C. 6.3.1.2) is a key enzyme of plant nitrogen metabolism that assimilates ammonia into glutamine. The Arabidopsis thaliana genome encodes one chloroplastic (GLN2) and five cytosolic (GLN1;1 – GLN1;5) isoforms with different expression patterns, kinetic properties, regulation and functions. Physiological roles of different isoforms have been elucidated mainly by studying knockout mutants. However, the role of GLN1;5, which is expressed in dry seeds, remains unknown. To clarifty the function of GLN1;5, we studied a GLN1;5 knockout line (GLN1;5KO) homozygous for T-DNA insertion within the GLN1;5. GLN1;5 deficiency results in a phenotype with slightly delayed bolting and fewer siliques. The dry weight of GLN1;5KO seeds was 73.3% of wild-type (WT) seed weight, with seed length 90.9% of WT seeds. Finally, only 18.33% of the mutant seeds germinated in water within 10 days in comparison to 34.67% of WT seeds. KNO3 strongly stimulated germination of both GLN1;5KO and WT seeds, while germination in the presence of increasing NH4Cl concentrations potentiated the differences between the two genotypes. It can be concluded that GLN1;5 activity supports silique development and grain filling and that it has a role in ammonium reassimilation in the seed, as well as assimilation and/or detoxification of ammonium from the environment.

https://doi.org/10.2298/ABS190315026D

Received: March 15, 2019; Accepted: April 4, 2019; Published online: April 10, 2019

How to cite this article: Dragićević MB, Ćuković KB, Zdravković-Korać SR, Simonović AD, Bogdanović MD, Todorović SI. Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach. Arch Biol Sci. 2019;71(3):443-53.

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Author Biography

Snežana R. Zdravković-Korać, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bul. despota Stefana 142, 11000 Belgrade


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Published

2019-10-23

How to Cite

1.
Dragićević MB, Ćuković KB, Zdravković-Korać SR, Simonović AD, Bogdanović MD, Todorović SI. Elucidation of the role of glutamine synthetase seed isoform GLN1;5 in Arabidopsis thaliana (L.) with a reverse genetics approach. Arch Biol Sci [Internet]. 2019Oct.23 [cited 2022Sep.29];71(3):443-5. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/4099

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