Enrichment of Cxcl12 promoter with TET2: a possible link between promoter demethylation and enhanced gene expression in the absence of PARP-1

Authors

  • Anja Z. Tolić Department of Molecular Biology, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0003-4703-6214
  • Jovana J. Rajić Department of Molecular Biology, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-2808-8484
  • Marija B. Đorđević Department of Molecular Biology, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0001-9051-9583
  • Miloš M. Đorđević Department of Molecular Biology, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0001-9951-1932
  • Svetlana S. Dinić Department of Molecular Biology, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-7612-3117
  • Nevena M. Grdović Department of Molecular Biology, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0003-1504-4578
  • Jelena D. Arambašić Jovanović Department of Molecular Biology, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-8810-0668
  • Mirjana V. Mihailović Department of Molecular Biology, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-8097-1186
  • Goran Đ. Poznanović Department of Molecular Biology, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0003-0839-7017
  • Tomasz P. Jurkowski 1. Institute of Biochemistry, University of Stuttgart, Pfaffenwaldring 55, 70569 Stuttgart, Germany; 2. School of Biosciences, Cardiff University, Cardiff, Wales, Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AX, UK http://orcid.org/0000-0002-2012-0240
  • Melita S. Vidaković Department of Molecular Biology, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0001-8410-6264
  • Aleksandra S. Uskoković Department of Molecular Biology, Institute for Biological Research “Siniša Stanković“ – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-9616-1102

Keywords:

DNA demethylation, 5-aza, TET2, PARP-1, CXCL12

Abstract

Abstract: Previously, we described the link between C-X-C motif chemokine 12 (Cxcl12) gene induction and DNA hypomethylation in the absence of poly(ADP-ribose) polymerase 1 (PARP-1). We have now firmly established that demethylation is the primary cause of gene induction on the basis of Cxcl12 gene upregulation upon treatment with the demethylating agent 5-azacytidine (5-aza). Since the demethylation state of Cxcl12 is favored by PARP-1 absence, we investigated the presence of ten-eleven translocation (TET) proteins on the Cxcl12 promoter in order to corroborate the relationship between the demethylation process and increased gene expression that occurs in the absence of PARP-1. Analysis was performed on the promoter region within CpG islands of Cxcl12 from control mouse embryonic fibroblasts (NIH3T3) and Parp-1 knock-out mouse embryonic fibroblasts (PARP1-/-). The lack of PARP-1 increased the abundance of TET2 on the Cxcl12 promoter, suggesting that TET-mediated demethylation provoked by the absence of PARP-1 could account for the observed increased expression of this chemokine. Deciphering the regulation of DNA (de)methylation factors that control Cxcl12 expression may provide an additional therapeutic approach in pharmacological interventions where gene switching on or off based on targeted stimulation or inhibition is necessary.

https://doi.org/10.2298/ABS190404027T

Received: April 4, 2019; Accepted: April 18, 2019; Published online: April 18, 2019

How to cite this article: Tolić AZ, Rajić JJ, Đorđević MB, Đorđević MM, Dinić SS, Grdović NM, Arambašić-Jovanović JD, Mihailović MV, Poznanović GĐ, Jurkowski TP, Vidaković MS, Uskoković AS.  Enrichment of Cxcl12 promoter with TET2: A possible link between promoter demethylation and enhanced gene expression in the absence of PARP-1. Arch Biol Sci. 2019;71(3):455-62.

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Published

2019-10-23

How to Cite

1.
Tolić AZ, Rajić JJ, Đorđević MB, Đorđević MM, Dinić SS, Grdović NM, Arambašić Jovanović JD, Mihailović MV, Poznanović G Đ., Jurkowski TP, Vidaković MS, Uskoković AS. Enrichment of Cxcl12 promoter with TET2: a possible link between promoter demethylation and enhanced gene expression in the absence of PARP-1. Arch Biol Sci [Internet]. 2019Oct.23 [cited 2024Oct.9];71(3):455-62. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/4152

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