Camellia sinensis leaf extracts lacking catechins exert depigmentary effects through ERK-dependent, MiTF-mediated tyrosinase downregulation in melan-a cells and a human skin equivalent

Authors

  • Donghyun Kim Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi do 17104
  • Nok Hyun Park Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si
  • Jeong Ah Hwang Amorepacific R&D Center, Yongin-si
  • Jeongkee Kim Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si
  • Yong-Joo Na Amorepacific R&D Center, Yongin-si
  • Jae Sung Hwang Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si
  • Chang Seok Lee Department of Beauty and Cosmetic Science, Eulji University, Seongnam-si
  • Deok Chun Yang 1. Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi do 17104; 2. Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si

Keywords:

Camellia sinensis, catechins, melanogenesis, tyrosinase

Abstract

Paper description:

  • Green tea (Camelia sinensis) polyphenol catechins have been studied extensively for their health benefits and for their anti-melanogenic properties. Bioactive components such as polysaccharides, amino acids and lipids have received little attention.
  • We examined the anti-melanogenic properties of tea leaf extract, which is mostly comprised of polysaccharides.
  • We describe the anti-melanogenesis mechanism by melan-a cells and a human skin equivalent. The fraction of tea extract without catechins regulates melanin synthesis by ERK-dependent degradation of MiTF and subsequent downregulation of tyrosinase expression.
  • C. sinensis leaf extracts without catechins could be developed as a new skin-lightening agent.


Abstract: Green tea from Camellia sinensis, a popular beverage worldwide, is also considered a herbal medicine. The bioactive compounds of green tea include polyphenols, polysaccharides, amino acids, and vitamins. Tea polyphenols are composed of various catechins such as epigallocatechin-3-gallate (EGCG), whereas polysaccharides include complex pectic substances and glycoproteins. Unfractionated C. sinensis leaf extracts show various pharmacological effects that are attributable to catechins, which include antimelanogenic properties. Most studies have focused on the biological function of catechins in green tea, and the effects of C. sinensis leaf extracts lacking catechins (CSLE-LC) have received little research attention. We examined the skin-whitening properties of the fraction lacking catechins. The melanin content in melan-a cells was significantly reduced, as has been shown for unfractionated C. sinensis extracts and catechins. We also elucidated the molecular mechanism underlying the antimelanogenic effects of CSLE-LC on skin melanocytes. Our results show that CSLE-LC acts through inhibition of MiTF and subsequent activation of extracellular-signal regulated kinase (ERK) to reduce tyrosinase protein levels. We confirmed the whitening ability of CSLE-LC using a human skin equivalent. Our findings provide the first evidence that CSLE-LC could exert efficient antimelanogenesis activity, and suggest that polysaccharides as well as catechins contribute to the whitening efficacy of C. sinensis leaf extracts.

https://doi.org/10.2298/ABS190510031K

Received: May 10, 2019; Revised: May 23, 2019; Accepted: May 24, 2019; Published online: May 28, 2019

How to cite this article: Kim D, Park NH, Hwang JA, Kim J, Na YJ, Hwang JS, Lee CS, Yang DC. Camellia sinensis leaf extracts lacking catechins exert depigmentary effects through ERK-dependent, MiTF-mediated tyrosinase downregulationin melan-a cells and a human skin equivalent. Arch boil Sci. 2019;71(3):483-8.

Downloads

Download data is not yet available.

References

Reygaert WC. Green Tea Catechins: Their Use in Treating and Preventing Infectious Diseases. Biomed Res Int. 2018;2018:9105261.

Janssens PL, Hursel R and Westerterp-Plantenga MS. Nutraceuticals for body-weight management: The role of green tea catechins. Physiol Behav. 2016;162:83-7.

Afaq F, Adhami VM, Ahmad N, Mukhtar H. Inhibition of ultraviolet B-mediated activation of nuclear factor kappaB in normal human epidermal keratinocytes by green tea Constituent (-)-epigallocatechin-3-gallate. Oncogene. 2003;22(7):1035-44.

Broadhurst CL, Polansky MM, Anderson RA. Insulin-like biological activity of culinary and medicinal plant aqueous extracts in vitro. J Agric Food Chem. 2000;48(3):849-52.

Cho SY, Park PJ, Shin HJ, Kim YK, Shin DW, Shin ES, Lee HH, Lee BG, Baik JH, Lee TR. (-)-Catechin suppresses expression of Kruppel-like factor 7 and increases expression and secretion of adiponectin protein in 3T3-L1 cells. Am J Physiol Endocrinol Metab. 2007;292(4):E1166-72.

Kao YH, Hiipakka RA, Liao S. Modulation of endocrine systems and food intake by green tea epigallocatechin gallate. Endocrinology. 2000;141(3):980-7.

A Waheed, FS Hasid, N Ahmad, B Mand Khan. An Over View of Tea Plantation in Pakistan. Asian J Plant Sci. 2002:1(4):495-8.

Camila TS, Lauro MD, Yanna DR, Nessana D, Simone MMP, Guilherme L, Philip AJG, Marcello I. Polysaccharides from green and black teas and their protective effect against murine sepsis. Food Res Int. 2013;53(2):780-5.

Xu P, Wu J, Zhang Y, Chen H, Wang YF. Physicochemical characterization of puerh tea polysaccharides and their antioxidant and alpha-glycosidase inhibition. J Funct Foods. 2014;6:545-54.

Wang YF, Shao SH, Xu P, Chen H, Lin-Shiau SY, Deng YT, Lin JK. Fermentation process enhanced production and bioactivities of oolong tea polysaccharides. Food Res Int. 2012;46(1):158-66.

Swalwell H, Latimer J, Haywood RM, Birch-Machin MA. Investigating the role of melanin in UVA/UVB- and hydrogen peroxide-induced cellular and mitochondrial ROS production and mitochondrial DNA damage in human melanoma cells. Free Radic Biol Med. 2012;52:626-34.

Briganti S, Camera E, Picardo M. Chemical and instrumental approaches to treat hyperpigmentation. Pigment Cell Res. 2003;16:101-10.

Hearing VJ, Tsukamoto K. Enzymatic control of pigmentation in mammals. FASEB J. 1991;5(14):2902-9.

Kim YC, Choi SY, Park EY. Anti-melanogenic effects of black, green, and white tea extracts on immortalized melanocytes. J Vet Sci. 2015;16(2):135-43.

Kim DS, Park SH, Kwon SB, Li K, Youn SW, Park KC. (-)-Epigallocatechin-3-gallate and hinokitiol reduce melanin synthesis via decreased MITF production. Arch Pharm Res. 2004;27(3):334-9.

Sato K, Toriyama M. Depigmenting effect of catechins. Molecules. 2009;14(11):4425-32.

Wu M, Hemesath TJ, Takemoto CM, Horstmann MA, Wells AG, Price ER, Fisher DZ, Fisher DE. c-Kit triggers dual phosphorylations, which couple activation and degradation of the essential melanocyte factor Mi. Genes Dev. 2000;14(3):301-12.

Xu W, Gong L, Haddad MM, Bischof O, Campisi J, Yeh ET, Medrano EE. Regulation of microphthalmia-associated transcription factor MiTF protein levels by association with the ubiquitin-conjugating enzyme hUBC9. Exp Cell Res. 2000;15;255(2):135-43.

Kim HS, Quon MJ, Kim JA. New insights into the mechanisms of polyphenols beyond antioxidant properties; lessons from the green tea polyphenol, epigallocatechin 3-gallate. Redox Biol. 2014;2:187-95.

Du LL, Fu QY, Xiang LP, Zheng XQ, Lu JL, Ye JH, Li QS, Polito CA, Liang YR. Tea Polysaccharides and Their Bioactivities. Molecules. 2016;21(11):E1449.

Downloads

Published

2019-10-23

How to Cite

1.
Kim D, Park NH, Hwang JA, Kim J, Na Y-J, Hwang JS, Lee CS, Yang DC. Camellia sinensis leaf extracts lacking catechins exert depigmentary effects through ERK-dependent, MiTF-mediated tyrosinase downregulation in melan-a cells and a human skin equivalent. Arch Biol Sci [Internet]. 2019Oct.23 [cited 2024Nov.24];71(3):483-8. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/4236

Issue

Vol. 71 No. 3 (2019)

Section

Articles

License

Authors grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.