Molecular characterization of <i>mariner</i>-like elements in <i> Bruchus pisorum</i> and <i>Bruchus rufimanus</i> (Coleoptera: Bruchidae)

Authors

  • Salma Djebbi Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis
  • Wiem Ben Amara Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis
  • Hanem Makni 1. Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis; 2. Institut Supérieur de l’Animation pour la Jeunesse et la Culture (ISAJC), Université de Tunis, Bir El Bey, Tunisia
  • Mohamed Makni Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis
  • Maha Mezghani-Khemakhem Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis

Keywords:

Mariner-like element, Coleoptera, Bruchus pisorum, B. rufimanus, mauritiana subfamily

Abstract

Mariner-like elements (MLEs) are Class-II transposons that are widely present in diverse organisms and encode a D,D34D transposase motif. MLE sequences from two coleopteran species, Bruchus pisorum and B. rufimanus were obtained using the terminal-inverted repeats (TIRs) of mariner elements belonging to the mauritiana subfamily as primer. The characterized elements were between 1073 and 1302 bp in length and are likely to be inactive, based on the presence of multiple stop codons and/or frameshifts. A single consensus of MLE was detected in B. pisorum and was named Bpmar1. This element exhibited several conserved amino acid blocks as well as the specific D,D(34)D signature. As for B. rufimanus, two MLE consensuses, designated Brmar1 and Brmar2, were isolated, both containing deletions overlapping the internal region of the transposase. Structural and phylogenetic analysis of these sequences suggested a relatively recent origin of Bpmar1 versus a more ancient invasion of Brmar1 and Brmar2 in their respective host genomes. Given that MLEs are potential mediators of insect resistance and have been used as vectors to transfer genes into host genomes, the MLEs characterized in this study will have valuable implications for selecting appropriate transposable elements in transgenesis.

https://doi.org/10.2298/ABS160407111D

Received: April 7, 2016; Revised: July 23, 2016; Accepted: July 26, 2016; Published online: November 9, 2016

How to cite this article: Djebbi S, Ben Amara W, Makni Hanem, Makni M, Mezghani-Khemakhem M. Molecular characterization of mariner-like elements in Bruchus pisorum and Bruchus rufimanus (Coleoptera: Bruchidae). Arch Biol Sci. 2017;69(2):353-60.

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Author Biographies

Salma Djebbi, Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis

Department of Biology

Wiem Ben Amara, Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis

Department of Biology

Mohamed Makni, Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis

Department of Biology

Maha Mezghani-Khemakhem, Unité de Recherche Génomique des Insectes Ravageurs des Cultures d’intérêt agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 El Manar, Tunis

Department of Biology

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2017-05-25

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Djebbi S, Ben Amara W, Makni H, Makni M, Mezghani-Khemakhem M. Molecular characterization of &lt;i&gt;mariner&lt;/i&gt;-like elements in &lt;i&gt; Bruchus pisorum&lt;/i&gt; and &lt;i&gt;Bruchus rufimanus&lt;/i&gt; (Coleoptera: Bruchidae). Arch Biol Sci [Internet]. 2017May25 [cited 2024Mar.28];69(2):353-60. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/433

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