Optimization of Bacillus aerius strain JS-786 cell dry mass and its antifungal activity against Botrytis cinerea using response surface methodology

Authors

  • Jamil Shafi Department of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110161, Liaoning
  • Ji Mingshan Department of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110161, Liaoning
  • Qi Zhiqiu Department of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110161, Liaoning
  • Li Xiuwei Department of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110161, Liaoning
  • Gu Zumin Department of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110161, Liaoning
  • Li Xinghai Department of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110161, Liaoning
  • Zhang Yang Department of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110161, Liaoning
  • Qin Peiwen Department of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110161, Liaoning
  • Tian Hongzhe Department of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110161, Liaoning
  • Che Wunan Department of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110161, Liaoning
  • Wang Kai Department of Pesticide Science, Plant Protection College, Shenyang Agricultural University, Shenyang 110161, Liaoning

Keywords:

antifungal activity, Bacillus aerius, Botrytis cinerea, cell dry mass, response surface methodology

Abstract

The optimization of fermentation conditions is necessary for field application of biological control agents. The present study was designed to optimize the fermentation conditions for the Bacillus aerius strain, JS-786 in terms of cell dry mass and its antifungal activity against Botrytis cinerea with response surface methodology. A strain of bacteria with strong antifungal activity was isolated from the phyllosphere of tomato plant and identified as B. aerius JS-786 based on the sequence homology of its 16S rRNA gene. After the success of preliminary antifungal activity tests, response surface methodology was used to optimize the fermentation conditions (medium pH, gelatin percentage, incubation period, rotatory speed and incubation temperature) to maximize the cell dry mass and antifungal activity against B. cinerea. A 25 factorial central composite design was employed and multiple response optimization was used to determine the desirability of the operation. The results of regression analysis showed that at the individual level, all of the experimental parameters were significant for cell dry mass; significant results were obtained for antifungal activity pH, incubation period, rotatory speed and incubation temperature. The interactive effect of the incubation period, rotatory speed and incubation temperature was significant. Maximum cell dry mass (8.7 g/L) and inhibition zone (30.4 mm) were obtained at pH 6.4, gelatin 3.2%, incubation period 36.92 h, rotatory speed 163 rpm, and temperature 33.5°C. This study should help to formulate a more rational and cost-effective biological product both in terms of bacterial growth and antifungal activity.

https://doi.org/10.2298/ABS160421122S

Received: April 21, 2016; Revised: May 27, 2016; Accepted: June 17, 2016; Published online: November 7, 2016

How to cite: Shafi J, Mingshan J, Zhiqiu Q, Xiuwei L, Zumin G, Xinghai L, Yang Z, Peiwen Q, Hongzhe T, Wunan C, Kai W. Optimization of bacillus aerius strain JS-786 cell dry mass and its antifungal activity against botrytis cinerea using response surface methodology. Arch Biol Sci. 2017;69(3):469-80.

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References

Prins TW, Tudzynski P, von Tiedemann A, Tudzynski B, Have AT, Hansen ME, Tenberge K, van Kan JAL. Infection strategies of Botrytis cinerea and related necrotrophic pathogens. In: Kronstad JW, editor. Fungal Pathology. 1st ed. Dordrecht: Springer; 2000. p. 33-64.

Abro MA. Nitrogen fertilization of the host plant influences susceptibility, production and aggressiveness of Botrytis cinerea secondary inoculum and on the efficacy of biological control. Phytopathol. 2013;103(3);261-7.

Elad Y, Williamson B, Tudzynski PN, Delen N. Botrytis spp. and diseases they cause in agricultural systems an introduction. In: Elad Y, Williamson B, Tudzynski PN, Delen N, editors. Botrytis: Biology, pathology and control. 1st ed. Dordrecht: Springer; 2007. p. 1-8.

Zhao M Tan H. Boty-II, a novel LTR retrotransposon in Botrytis cinerea B05. 10 revealed by genomic sequence. Electron J Biotechnol. 2009;12(3):2-3.

Genoscope. Botrytis cinerea, estimated losses for vineyards in France amount to 15-40% of the harvest, depending on climatic conditions. [cited 2016 Feb 24]. In: Genoscope: a online site [Internet]. France: Centre National de Sequencage. 2008- . [about 3 p.] Available: http://www.genoscope.cns.fr/spip/Botrytis-cinerea-estimated-losses.

Zhu H, Huang CT, Mingshan J. Baseline sensitivity and control efficacy of pyrisoxazole against Botrytis cinerea. Eur J Plant Pathol. 2016;146(2):315-23.

Marin DH, Romero RA, Guzman MSutton TB. Black Sigatoka: an increasing threat to banana cultivation. Plant Dis. 2003;87(3):208-22.

Bailey K, Boyetchko S, Längle T. Social and economic drivers shaping the future of biological control: a Canadian perspective on the factors affecting the development and use of microbial biopesticides. Biol Control. 2010;52(3):221-9.

Raaijmakers JM, De Bruijn I, Nybroe OM. Natural functions of lipopeptides from Bacillus and Pseudomonas: more than surfactants and antibiotics. FEMS Microbiol Rev. 2010;34(6):1037-62.

Heins SD, Manker DC, Jimenez DR, McCoy RJ, Marrone PGOrjala JE, inventors; AgraQuest, Inc., assignee. Compositions and methods for controlling plant pests. United States patent US 6,103,228. 2000 Aug 15.

McSpadden G, Brain B. Ecology of Bacillus and Paenibacillus spp. in agricultural systems. Phytopathol. 2004;94(11):1252-8.

Swain M, Ray R. Biocontrol and other beneficial activities of Bacillus subtilis isolated from cowdung microflora. Microbiol Res. 2009;164(2):121-30.

Ajilogba CF, Babalola OOAhmad F. Antagonistic Effects of Bacillus Species in Biocontrol of Tomato Fusarium Wilt. Stud Ethno-Med. 2013;7(3):205-16.

Hynes RK, Boyetchko SM. Research initiatives in the art and science of biopesticide formulations. Soil Biol Biochem. 2006;38(4):845-9.

Schisler D, Slininger P, Behle RM. Formulation of Bacillus spp. for biological control of plant diseases. Phytopathol. 2004;94(11):1267-71.

Nunes CA. Biological control of postharvest diseases of fruit. Eur J Plant Pathol. 2012; 133(1):181-96.

Kennedy M, Krouse D. Strategies for improving fermentation medium performance: a review. J Ind Microbiol. 1999;23(6):456-75.

Grahovac J, Grahovac M, Dodić J, Bajić BJ. Optimization of cultivation medium for enhanced production of antifungal metabolites by Streptomyces hygroscopicus. Crop Protect. 2014;65:143-52.

Kong Y, Zou P, Miao L, Qi J, Song L, Zhu LXu X. Medium optimization for the production of anti-cyanobacterial substances by Streptomyces sp. HJC-D1 using response surface methodology. Environ Sci Pollut Res. 2014;21(9):5983-90.

Lin Y, Du D, Si C, Zhao Q, Li ZLi P. Potential biocontrol Bacillus sp. strains isolated by an improved method from vinegar waste compost exhibit antibiosis against fungal pathogens and promote growth of cucumbers. Biol Control. 2014;71:7-15.

Tamura K, Dudley J, Nei MKS. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24(8):1596-99.

Flores M, Ford EGJanda JM. Value of the O-nitrophenyl-beta-D-galactopyranoside test to differentiate among the aerobic actinomycetes. J Clin Microbiol. 1990;28(9):2142-4.

Atehortúa P, Álvarez HOrduz S. Modeling of growth and sporulation of Bacillus thuringiensis in an intermittent fed batch culture with total cell retention. Bioprocess Biosystems Eng. 2007;30(6):447-56.

Mosquera S, González-Jaramillo LM, Orduz EV. Multiple response optimization of Bacillus subtilis EA-CB0015 culture and identification of antifungal metabolites. Biocatal Agric Biotechnol. 2014;3(4):378-85.

Wang YH, Feng JT, Zhang QX. Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology. J Appl Microbiol. 2008;104(3):735-44.

Bonev B, Hooper JPJ. Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method. J Antimicrob Chemother. 2008;61(6):1295-301.

Oh BY. Pesticide Residues for Food Safety and Environment Protection. [cited 2016 Nov 11]. In: FFTC document database [internet] .Taiwan: Food & Fertilizer Technology Center. 2001. [about 12 p.] Avaiable from: http://www.fftc.agnet.org/htmlarea_file/library/20110715225622/eb495.pdf

Akhurst R. Antibiotic activity of Xenorhabdus spp., bacteria symbiotically associated with insect pathogenic nematodes of the families Heterorhabditidae and Steinernematidae. J Gen Microbiol. 1982;128(12):3061-5.

Chen G, Maxwell P, Dunyhy GWJ. Culture conditions for Xenorhabdus and Photorhabdus symbionts of entomopathogenic nematodes. Nematologica. 1996;42(1):124-30.

Wan AM, Chen XMHZ. Wheat stripe rust in China. Crop Pasture Sci. 2007;58(6):605-19.

Wang YH, Zhang X. Influence of agitation and aeration on growth and antibiotic production by Xenorhabdus nematophila. World J Microbiol Biotechnol. 2007;23(2):221-7.

Younis MA, Hezayen FF, Nour EMA, Shabeb MS. Optimization of cultivation medium and growth conditions for Bacillus subtilis KO strain isolated from sugar cane molasses. Am Eurasian J Agric Environ Sci. 2010;7(1):31-7.

Buckland B, Gbewonyo K, Hallada T, Kaplan LMP. Production of lovastatin, an inhibitor of cholesterol accumulation in humans. In: Demain AL, Somkuti GA, Hunter- Cevera JC, Rossmore HW, ediors. Novel microbial products for medicine and agriculture. Amsterdam: Elsevier; 1989. p. 161-9.

Yang X, Yang H, Jian HLZ. Effect of fermentation conditions on antibiotic production of Xenorhabdus nematophilus. Wei sheng wu xue tong bao. 2000;28(1):12-6.

Choma C, Clavel T, Dominguez H, Razafindramboa N, Soumille H, Nguyen-the CSchmitt P. Effect of temperature on growth characteristics of Bacillus cereus TZ415. Int J Food Microbiol. 2000;55(1):73-7.

Nedwell D. Effect of low temperature on microbial growth: lowered affinity for substrates limits growth at low temperature. FEMS Microbiol Ecol. 1999;30(2):101-11.

Nastro R, Di CA, Gesuele R, Trifuoggi M, Inglese MGM. Influence of temperature on the production of antibiotic molecules in Bacillus amyloliquefaciens strain HNA3. In: Méndez-Vilas A, editor. Science against microbial pathogens: communicating current research and technological advances. Badajoz: Formatex; 2011. p. 1307-10. (Microbiology Series; vol. 3).

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Published

2017-08-11

How to Cite

1.
Shafi J, Mingshan J, Zhiqiu Q, Xiuwei L, Zumin G, Xinghai L, Yang Z, Peiwen Q, Hongzhe T, Wunan C, Kai W. Optimization of Bacillus aerius strain JS-786 cell dry mass and its antifungal activity against Botrytis cinerea using response surface methodology. Arch Biol Sci [Internet]. 2017Aug.11 [cited 2024Nov.21];69(3):469-80. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/479

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Vol. 69 No. 3 (2017)

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