Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro

Authors

  • Tatjana Ćosić Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-2715-0944
  • Jelena Savić Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-8385-7549
  • Martin Raspor Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-7014-9818
  • Aleksandar Cingel Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-8869-0020
  • Nabil Ghalawnji Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade
  • Branka Vinterhalter Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0001-8502-7285
  • Slavica Ninković Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-9394-3854

Keywords:

kohlrabi tissue culture, sugars, plant growth regulators, morphogenesis, de novo shoot formation

Abstract

Paper description:

  • Kohlrabi (Brassica oleracea var. gongylodes) with its edible stem tuber formed at the base of plant stem, presents a valuable source of nutrients.
  • The effects of plant growth regulators and different concentrations of various sugars on kohlrabi in vitro development and tuber formation were studied.
  • Growth and development of treated kohlrabi seedlings was highly affected in a distinctive manner with altered morphological traits. Plant growth regulators stimulated callus formation and de novo organogenesis.
  • Further investigation of the underlying mechanisms is necessary for understanding stem swelling in kohlrabi and involvement of the carbon source, plant hormones and/or other factors.


Abstract: Kohlrabi (Brassica oleracea var. gongylodes), with its edible stem tuber formed at the base of the plant stem, presents a valuable source of nutrients. The potential effects of plant growth regulators (PGRs), as well as various concentrations of different sugars on the in vitro development of kohlrabi were studied. Ten-day-old kohlrabi seedlings were cultivated in vitro for 5 weeks at 18±2°C on half-strength MS media containing different concentrations of carbon source such as sucrose, fructose, glucose, xylose and mannitol, combined with or without specific plant growth regulators (N6-benzyladenine (BA), gibberellic acid (GA3), 2,3,5-triiodobenzoic acid (TIBA)). Results showed no tuber formation in all treatments, but growth and development of treated kohlrabi seedlings was significantly affected in a distinctive manner, with a variety of morphological traits being altered in comparison to matching controls.

https://doi.org/10.2298/ABS200622029C

Received: June 22, 2020; Accepted: July 1, 2020; Published online: July 14, 2020

How to cite this article: Ćosić T, Savić J, Raspor M, Cingel A, Ghalawnji N, Vinterhalter B, Ninković S. Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro. Arch Biol Sci. 2020;72(3):349-57.

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Author Biographies

Jelena Savić, Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade


 

 

Martin Raspor, Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade


 

 

 

Aleksandar Cingel, Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade


 

 

Nabil Ghalawnji, Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade


 

Branka Vinterhalter, Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade


 

 

Slavica Ninković, Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade


 

 

 

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Published

2020-10-19

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1.
Ćosić T, Savić J, Raspor M, Cingel A, Ghalawnji N, Vinterhalter B, Ninković S. Effects of different types of sugars and plant growth regulators on kohlrabi seedling growth and development in vitro. Arch Biol Sci [Internet]. 2020Oct.19 [cited 2024Dec.27];72(3):349-57. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/5530

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Vol. 72 No. 3 (2020)

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