Effect of hydric and light stress on biomass, nutrient uptake and enzymatic antioxidants of Argania spinosa seedlings

Authors

  • Abdouroihamane Hachemi Laboratory of Pharmacology, Neurobiology, Anthropobiology and Environment, Faculty of Sciences Semlalia, Cadi Ayyad University, 40000 Marrakesh
  • Ouswati Said Ali Laboratory of Pharmacology, Neurobiology, Anthropobiology and Environment, Faculty of Sciences Semlalia, Cadi Ayyad University, 40000 Marrakesh https://orcid.org/0000-0002-3798-8054
  • Tarik Belghazi Regional Forestry Research Center of Marrakesh, 40000 Marrakesh https://orcid.org/0000-0002-0436-4608
  • Abderrahman Lahrouni Laboratory of Fluid Mechanics and Energetics, Faculty of Sciences Semlalia, Cadi Ayyad University, 40000 Marrakesh https://orcid.org/0000-0002-2118-8570
  • Said El Mercht Regional Forestry Research Center of Marrakesh, 40000 Marrakesh
  • Chakib El Hassan Regional Forestry Research Center of Marrakesh, 40000 Marrakesh https://orcid.org/0000-0001-7063-5675
  • Said El Messoussi Laboratory of Pharmacology, Neurobiology, Anthropobiology and Environment, Faculty of Sciences Semlalia, Cadi Ayyad University, 40000 Marrakesh https://orcid.org/0000-0002-9557-2196

DOI:

https://doi.org/10.2298/ABS201220010H

Keywords:

Argania spinosa, water stress, sunlight, macroelement uptake, shading

Abstract

Paper description:

  • In semi-arid ecosystems the success of a plantation of forest species such as Argania spinosa depends on the morphological and physiological qualities and the resistance capacity of seedlings to transplantation stress.
  • The interactive effects of acclimatization of spinosa to water and light stress on biomass, nutrient uptake and antioxidant enzyme activities were evaluated in the nursery.
  • Argan tree seedlings grown under 60% of sunlight showed a significant increase in total biomass, nutrient absorption and decreased enzyme activity.
  • This indicates an ability to mitigate the adverse effects of water stress and effectively prevent stress caused by excess light.

Abstract: Sunlight and water are factors that affect seedling development. However, the effects of acclimatization of seedlings to sunlight and water stress remain poorly understood. This study aimed to examine the interactive effects of acclimatization to light and water stress on argan tree (Argania spinosa) seedlings in nurseries. An experiment was conducted with 504 seedlings using two sunlight treatments (L1 and L2, i.e. 100% and 60% of full sunlight, respectively), and three watering treatments (well-watered, moderate stress and severe stress, i.e. 100%, 50% and 25% of field capacity, respectively). According to our results, water stress treatments caused a reduction in total biomass accumulation, nitrogen and phosphorus uptake. Water stress significantly increased other macroelements, H2O2 and MDA levels and antioxidant enzyme activities compared to well-watered seedlings. Seedlings grown under moderate shade (L2) showed higher macroelement uptake, which probably contributed to the increase in total biomass in all water treatments. The highest membrane stability index (MSI) values, H2O2 and MDA levels and lowest antioxidant enzyme activities were recorded in acclimatized argan seedlings under moderate shading (L2). These results suggest that moderate shade can effectively prevent stress caused by light excess and can also mitigate the harmful effects of water stress on A. spinosa seedlings.

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References

Defaa C, Elantry S, El Alami SL, Achour A, El Mousadik A, Msanda F. Effects of Tree Shelters on the Survival and Growth of Argania spinosa Seedlings in Mediterranean Arid Environment. Int J Ecol. 2015;2015:124075.

Zahidi A, Bani-Aameur F, El Mousadik A. Growth variability in Argania spinosa seedlings subjected to different levels of drought stress. J Hortic For. 2013;5(11):204‑17.

Struve DK, Ferrini F, Fini A, Pennati L. Relative growth and water use of seedlings from three Italian Quercus species. Arboric Urban For. 2009;35(3):113‑21.

Yang Y, Liu Q, Han C, Qiao YZ, Yao XQ, Yin HJ. Influence of water stress and low irradiance on morphological and physiological characteristics of Picea asperata seedlings. Photosynthetica. 2007;45(4):613‑9.

Bista DR, Heckathorn SA, Jayawardena DM, Mishra S, Boldt JK. Effects of drought on nutrient uptake and the levels of nutrient-uptake proteins in roots of drought-sensitive and -tolerant grasses. Plants. 2018;7(2): 1-16.

He M, Dijkstra FA. Drought effect on plant nitrogen and phosphorus: A meta-analysis. New Phytol. 2014;204(4):924‑31.

Chakhchar A, Wahbi S, Lamaouia M, Ferradous A, El Mousadik A, Ibnsouda-Koraichie S, Filali-Maltouf A, El Modafar C. Physiological and biochemical traits of drought tolerance in Argania spinosa. J Plant Interact. 2015;10(1):252‑61.

Smirnoff N. The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol. 1993;125(1):27‑58.

Lin J, Zhang R, Hu Y, Song Y, Hänninen H, Wu J. Interactive effects of drought and shading on Torreya grandis seedlings: physiological and growth responses. Trees Struct Funct. 2019;33(3):951‑61.

De Wit M, Galvão VC, Fankhauser C. Light-Mediated Hormonal Regulation of Plant Growth and Development. Annu Rev Plant Biol. 2016;67(February):513‑37.

Escobar-Bravo R, Klinkhamer PGL, Leiss KA. Interactive effects of UV-B light with abiotic factors on plant growth and chemistry, and their consequences for defense against arthropod herbivores. Front Plant Sci. 2017;8:1‑14.

Long SP, Humphries S. Photoinhibition of Photosynthesis in nature. Annu Rev Plant Physiol Plant Mol Biol. 1994;45:633‑62.

Azevedo GFC, Marenco RA. Growth and physiological changes in saplings of Minquartia guianensis and Swietenia macrophylla during acclimation to full sunlight. Photosynthetica. 2012;50(1):86‑94.

Quero JL, Villar R, Marañón T, Zamora R. Interactions of drought and shade effects on seedlings of four Quercus species: physiological and structural leaf responses. New Phytol. 2006;170:819‑34.

Chakhchar A, Lamaoui M, Wahbi S, Ferradous A, El Mousadik A, Ibnsouda-Koraichi S, Filali-Maltouf A, El Modafar C. Leaf water status, osmoregulation and secondary metabolism as a model for depicting drought tolerance in Argania spinosa. Acta Physiol Plant. 2015;37(4):1‑16.

Chakhchar A, Lamaoui M, Aissam S, Ferradous A, Wahbi S, El Mousadik A, Ibnsouda-Koraichi S, Filali-Maltouf A, El Modafar C. Electrolyte ions and glutathione enzymes as stress markers in Argania spinosa subjected to drought stress and recovery. Afr J Biotechnol. 2017;16(1):10‑21.

Kalra YP, Maynard DG. Méthodes d’analyse des sols forestiers et des tissus végétaux [Internet]. Edmonton (Alberta) : Forêts Canada, Région du Nord-Ouest ; Centre de Foresterie du Nord ; Rapport d’information NOR-X-319F ; 1992 [cited 2020 Dec 20]. 129 p. Available from: https://cfs.nrcan.gc.ca/publications?id=11844

Chakhchar A. Mécanismes physiologiques et biochimiques de la tolérance de l’arganier au stress hydrique. [dissertation]. [Morocco]: Université Cadi Ayyad, Faculté des Sciences Semlalia Marrakech; 2015. 210 p.

Velikova V, Yordanov I, Edreva A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants protective role of exogenous polyamines. Plant Sci. 2000;151(1):59‑66.

Hernández JA, Almansa MS. Short-term effects of salt stress on antioxidant systems and leaf water relations of pea leaves. Physiol Plant. 2002;115(2):251‑7.

Bradford MM. A Rapid and Sensitive Method for the Quantitation Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Anal Biochem. 1976;72:248‑54.

Beauchamp C, Fridovich I. Superoxide Dismutase: Improved Assays and an Assay Applicable to Acrylamide Gels. Anal Biochem. 1971;44:276‑87.

Zhang S, Lu S, Xu X, Korpelainen H, Li C. Changes in antioxidant enzyme activities and isozyme profiles in leaves of male and female Populus cathayana infected with Melampsora larici-populina. Tree Physiol. 2010;30(1):116‑28.

Moore BM, Flurkey WH. Sodium dodecyl sulfate activation of a plant polyphenol oxidase. Effect of sodium dodecyl sulfate on enzymatic and physical characteristics of purified broad bean polyphenoloxidase. J Biol Chem. 1990;265(9):4982‑8.

Gardiner ES, Hodges JD. Growth and biomass distribution of cherrybark oak (Quercus pagoda Raf.) seedlings as influenced by light availability. For Ecol Manage. 1998;108(1‑2):127‑34.

Hu Y, Burucs Z, von Tucher S, Schmidhalter U. Short-term effects of drought and salinity on mineral nutrient distribution along growing leaves of maize seedlings. Environ Exp Bot. 2007;60(2):268‑75.

Ahanger MA, Morad-Talab N, Abd-Allah EF, Ahmad P, Hajiboland R. Plant growth under drought stress: Significance of mineral nutrients. Water Stress and Crop Plants: A Sustainable Approach. 2016;2‑2:649‑68.

Tanguilig VC, Yambao EB, O’toole JC, De Datta SK. Water stress effects on leaf elongation, leaf water potential, transpiration, and nutrient uptake of rice, maize, and soybean. Plant Soil. 1987;103(2):155‑68.

Kirnak H, Tas I, Kaya C, Higgs D. Effects of deficit irrigation on growth, yield, and fruit quality of eggplant under semi-arid conditions. Aust J Agric Res. 2002;53(12):1367‑73.

Sánchez-Rodríguez E, del Mar Rubio-Wilhelmi M, Cervilla LM, Blasco B, Rios JJ, Leyva R, Romero L, Ruiz JM. Study of the ionome and uptake fluxes in cherry tomato plants under moderate water stress conditions. Plant Soil. 2010;335(1):339‑47.

Patakas A, Nikolaou N, Zioziou E, Radoglou K, Noitsakis B. The role of organic solute and ion accumulation in osmotic adjustment in drought-stressed grapevines. Plant Sci. 2002;163(2):361‑7.

Yuan yuan M, Wei yi S, Zi hui L, Hong mei Z, Xiu lin G, Hong bo S, Fu tai N. The dynamic changing of Ca2+ cellular localization in maize leaflets under drought stress. Comptes Rendus Biol. 2009;332(4):351‑62.

Wilkinson SR, Welch RM, Mayland HF, Grunes DL. Magnesium in plants: uptake, distribution, function, and utilization by man and animals. In: Sigel H, Sigel A, editors. Metal Ions in Biological Systems. New York, Basel: Marcel Dekker; 1990. p. 33‑56. (Compendium on Magnesium and Its Role in Biology, Nutrition, and Physiology; Vol. 26).

Talbi S, Romero-Puertas MC, Hernández A, Terrón L, Ferchichi A, Sandalio LM. Drought tolerance in a Saharian plant Oudneya africana: Role of antioxidant defences. Environ Exp Bot. 2015;111:114‑26.

Shi H, Ye T, Song B, Qi X, Chan Z. Comparative physiological and metabolomic responses of four Brachypodium distachyon varieties contrasting in drought stress resistance. Acta Physiol Plant. 2015;37(6):1‑12.

Foyer CH, Noctor G. Redox homeostasis and antioxidant signaling: A metabolic interface between stress perception and physiological responses. Plant Cell. 2005;17(7):1866‑75.

Khajeeyan R, Salehi A, Dehnavi MM, Farajee H, Kohanmoo MA. Physiological and yield responses of Aloe vera plant to biofertilizers under different irrigation regimes. Agric Water Manage. 2019;225:105768.

Ma X, Song L, Yu W, Hu Y, Liu Y, Wu J, Ying Y. Growth, physiological, and biochemical responses of Camptotheca acuminata seedlings to different light environments. Front Plant Sci. 2015;6(MAY):1‑12.

Jafarnia S, Akbarinia M, Hosseinpour B, Modarres Sanavi SA, Salami SA. Effect of drought stress on some growth, morphological, physiological, and biochemical parameters of two different populations of Quercus brantii. IForest. 2018;11(2):212‑20.

Feng L, Raza MA, Li Z, Chen Y, Khalid MH Bin, Du J, Liu W, Wu X, Song C, Yu L, Zhang Z, Yuan S, Yang W, Yang F. The influence of light intensity and leaf movement on photosynthesis characteristics and carbon balance of Soybean. Front Plant Sci. 2019;9:1952.

Santos C, Fragoeiro S, Phillips A. Physiological response of grapevine cultivars and a rootstock to infection with Phaeoacremonium and Phaeomoniella isolates: An in vitro approach using plants and calluses. Sci Hortic. 2005;103(2):187‑98.

Shanazari M, Golkar P, Mirmohammady Maibody AM. Effects of drought stress on some agronomic and bio-physiological traits of Triticum aestivum, Triticale, and Tritipyrum genotypes. Arch Agron Soil Sci. 2018;64(14):2005‑18.

Sayfzadeh S, Habibi D, Taleghani DF, Kashani A, Vazan S, Qaen SHS, Khodaei AH, Boojar MMA, Rashidi M. Response of antioxidant enzyme activities and root yield in sugar beet to drought stress. Int J Agric Biol. 2011;13(3):357‑62.

Gill SS, Tuteja N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem. 2010;48(12):909‑30.

Rivero RM, Ruiz JM, Garcı PC, Lo LR, Sa E, Romero L. Resistance to cold and heat stress: accumulation of phenolic compounds in tomato and watermelon plants. Plant Sci. 2001;160:315‑21.

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Published

2021-03-19

How to Cite

1.
Hachemi A, Said Ali O, Belghazi T, Lahrouni A, El Mercht S, El Hassan C, El Messoussi S. Effect of hydric and light stress on biomass, nutrient uptake and enzymatic antioxidants of Argania spinosa seedlings. Arch Biol Sci [Internet]. 2021Mar.19 [cited 2024Nov.22];73(1):145-53. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/6082

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Vol. 73 No. 1 (2021)

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