Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption

Authors

  • Ivan Radić Institute of Pathological Physiology, Faculty of Medical Science, University of Priština, Anri Dinana bb, 38220 Kosovska Mitrovica https://orcid.org/0000-0002-0657-8829
  • Mirjana Mirić Institute of Pathological Physiology, Faculty of Medical Science, University of Priština, Anri Dinana bb, 38220 Kosovska Mitrovica https://orcid.org/0000-0002-1172-4624
  • Milica Mijović Institute of Pathology, Faculty of Medical Science, University of Priština, Anri Dinana bb, 38220 Kosovska Mitrovica https://orcid.org/0000-0003-1884-6066
  • Nikola Tatalović Department of Physiology, Institute for Biological Research "Siniša Stanković" – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade https://orcid.org/0000-0002-0745-8845
  • Miloš Mitić Department of Molecular Biology and Endocrinology, „VINČA" Institute of Nuclear Sciences – National Institute of thе Republic of Serbia, University of Belgrade, Mike Petrovića Alasa 12-14, 11351 Vinča, Belgrade https://orcid.org/0000-0002-3849-6561
  • Vojkan Nestorović Institute of Physiology, Faculty of Medical Science, University of Priština, Anri Dinana bb, 38220 Kosovska Mitrovica https://orcid.org/0000-0003-0751-8163
  • Miroslav Adžić Department of Molecular Biology and Endocrinology, „VINČA" Institute of Nuclear Sciences – National Institute of thе Republic of Serbia, University of Belgrade, Mike Petrovića Alasa 12-14, 11351 Vinča, Belgrade https://orcid.org/0000-0003-4234-7275
  • Duško P. Blagojević Department of Physiology, Institute for Biological Research "Siniša Stanković" – National Institute of the Republic of Serbia, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade https://orcid.org/0000-0001-6338-2833
  • Ljiljana Popović Institute of Pathological Physiology, Faculty of Medical Science, University of Priština, Anri Dinana bb, 38220 Kosovska Mitrovica https://orcid.org/0000-0003-1165-9022
  • Snežana Janićijević Hudomal Institute of Pharmacology and Toxicology, Faculty of Medical Science, University of Priština, Anri Dinana bb, 38220 Kosovska Mitrovica https://orcid.org/0000-0001-5517-8250

DOI:

https://doi.org/10.2298/ABS201205008R

Keywords:

chronic alcohol consumption, alcoholic liver disease, pumpkin seed oil, antioxidant enzymes, NF-κB

Abstract

Paper description:

  • Long-term alcohol consumption leads to alcoholic liver disease (ALD) that can be prevented by the application of pumpkin seed oil (PSO).
  • Male albino rats were given PSO (per os, 2 mL/kg b.w./day) with 12% ethanol in water ad libitum for 6 weeks.
  • PSO reduced or eliminated the histopathological changes induced in the liver by chronic concomitant ethanol consumption.
  • The protective effect of PSO in ALD correlated with elevated NF-κB; the protective effect was mediated by NF-κB.

Abstract: Pumpkin seed oil (PSO) possesses a protective potential against liver injury due to the presence of biologically active ingredients. Adult male albino rats were administrated PSO (per os, 2 mL/kg b.w./day) and a 12% ethanol solution in water, ad libitum, with an average intake of 8.14 g of ethanol/kg bw/day for 6 weeks. Congestion, hepatic central vein dilation, portal vein branch dilation, Kupffer cell hyperplasia, fatty liver changes, hepatocyte focal necrosis were observed after daily alcohol intake. All observed changes were reduced when PSO was ingested with ethanol. PSO intake itself induced discrete cellular edema, congestion and slight dilatation of the central and portal vain branches. Chronic ethanol intake elevated catalase (CAT) activity and glutathione reductase (GR) protein expression; concomitant PSO intake had no effect on CAT activity or GR protein expression. PSO intake decreased the activities of GR, glutathione-S-transferase (GST) and xanthine oxidase (XOD) in the liver, probably due to the ingestion of antioxidants. Intake of PSO and ethanol significantly decreased cytosolic superoxide dismutase (SOD1) and increased NF-κB protein expression compared to ethanol intake, suggesting that the protective effects of PSO were mediated by the NF-κB signaling pathway. Our results reveal a therapeutic potential of PSO in alcoholic liver disease.

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Published

2021-03-19

How to Cite

1.
Radić I, Mirić M, Mijović M, Tatalović N, Mitić M, Nestorović V, Adžić M, Blagojević DP, Popović L, Janićijević Hudomal S. Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption. Arch Biol Sci [Internet]. 2021Mar.19 [cited 2024Nov.22];73(1):123-3. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/6170

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Vol. 73 No. 1 (2021)

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