MiR-548ar-3p increases cigarette smoke extract-induced chronic obstructive pulmonary disease (COPD) injury through solute carrier family 17 member 9 (SLC17A9)
DOI:
https://doi.org/10.2298/ABS220201008ZKeywords:
chronic obstructive pulmonary disease (COPD), cigarette smoke extract (CSE), miR-548ar-3p, solute carrier family 17 member 9 (SLC17A9), high-throughput sequencing, human bronchial epithelial cellsAbstract
Paper description:
- The pathogenesis of chronic obstructive pulmonary disease (COPD) caused by smoking is unclear.
- miR-548ar-3p was selected by high-throughput sequencing of peripheral blood from smoking (COPD) and non-smoking (normal) individuals. A miR-548ar-3p mimic was transfected into human bronchial epithelial (HBE) cells and a COPD model was established by treating HBE cells with cigarette smoke extract (CSE). The effect of miR-548ar-3p on cell proliferation, apoptosis and SLC17A9 protein expression was examined.
- miR-548ar-3p was expressed at a lower level in COPD patients. It caused decreased cell viablity, promoted apoptosis and increased SLC17A9 protein expression.
- miR-548ar-3p may aggravate CSE-induced COPD injury through SLC17A9.
Abstract: This study investigated the effect of microRNA mir-548ar-3p on cigarette smoke extract (CSE)-induced chronic obstructive pulmonary disease (COPD). High-throughput sequencing was performed on peripheral blood from smoking COPD patients and non-smoking individuals with normal pulmonary function, and miR-548ar-3p RNA, possessing large differential expression was selected. Experimental groups were divided into control, experimental model (EM), EM+mimic miRNA, negative control (NC) and EM+miR-548ar-3p groups; an empty vector or miR-548ar-3p mimic was transfected into human bronchial epithelial (HBE) cells. A COPD model was established by treating HBE cells with CSE. Cell viability, apoptosis and solute carrier family 17 member 9 (SLC17A9) protein expression were examined by cell counting kit-8, flow cytometry and Western blotting, respectively. Cell viability in the EM+miR-548ar-3p group decreased significantly, and the apoptosis rate and SLC17A9 protein expression increased significantly compared with the control (P<0.05, all groups). In smoking COPD patients, interferon (IFN)-γ and interleukin (IL)-17α expression detected by ELISA was significantly higher than in normal individuals. miR-548ar-3p expression was significantly lower (P<0.05, all groups). These findings suggest that miR-548ar-3p was expressed at a lower level in COPD patients. miR-548ar-3p may increase the extent of CSE-induced COPD injury through SLC17A9
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Mirza S, Clay RD, Koslow MA, Scanlon PD. COPD Guidelines: A Review of the 2018 GOLD Report. Mayo Clin Proc. 2018;93(10):1488-502. https://doi.org/10.1016/j.mayocp.2018.05.026
Brusselle GG, Joos GF, Bracke KR. New insights into the immunology of chronic obstructive pulmonary disease. Lancet. 2011;378(9795):1015-26. https://doi.org/10.1016/S0140-6736(11)60988-4
Farland MZ, Peters CJ, Williams JD, Bielak KM, Heidel RE, Ray SM. β-Blocker use and incidence of chronic obstructive pulmonary disease exacerbations. Ann Pharmacother. 2013;47(5):651-6. https://doi.org/10.1345/aph.1R600
Gautam SS, O'Toole RF. Convergence in the epidemiology and pathogenesis of COPD and pneumonia. COPD. 2016;13(6):790-8. Wouters EF. Nutrition and metabolism in COPD. Chest. 2000;117(5 Suppl 1):274S-80S. https://doi.org/10.1080/15412555.2016.1191456
Wouters EF. Nutrition and metabolism in COPD. Chest. 2000;117(5 Suppl 1):274S-80S. https://doi.org/10.1378/chest.117.5_suppl_1.274S
Zeng Z, He S, Lu J, Liu C, Lin H, Xu C, Xie L, Sun S. MicroRNA-21 aggravates chronic obstructive pulmonary disease by promoting autophagy. Exp Lung Res. 2018;44(2):89-97. https://doi.org/10.1080/01902148.2018.1439548
Jeon HS, Lee YH, Lee SY, Jang JA, Choi YY, Yoo SS, Lee WK, Choi JE, Son JW, Kang YM, Park JY. A common polymorphism in pre-microRNA-146a is associated with lung cancer risk in a Korean population. Gene. 2014;534(1):66-71. https://doi.org/10.1016/j.gene.2013.10.014
Sessa R, Hata A. Role of microRNAs in lung development and pulmonary diseases. Pulm Circ. 2013;3(2):315-28. https://doi.org/10.4103/2045-8932.114758
Tejero R, Navarro A, Campayo M, Viñolas N, Marrades RM, Cordeiro A, Ruíz-Martínez M, Santasusagna S, Molins L, Ramirez J, Monzó M. miR-141 and miR-200c as markers of overall survival in early stage non-small cell lung cancer adenocarcinoma. PLoS One. 2014;9(7):e101899. https://doi.org/10.1371/journal.pone.0101899
Zhang JG, Wang JJ, Zhao F, Liu Q, Jiang K, Yang GH. MicroRNA-21 (miR-21) represses tumor suppressor PTEN and promotes growth and invasion in non-small cell lung cancer (NSCLC). Clin Chim Acta. 2010;411(11-12):846-52. https://doi.org/10.1016/j.cca.2010.02.074
Dinh TK, Fendler W, Chałubińska-Fendler J, Acharya SS, O'Leary C, Deraska PV, D'Andrea AD, Chowdhury D, Kozono D. Circulating miR-29a and miR-150 correlate with delivered dose during thoracic radiation therapy for non-small cell lung cancer. Radiat Oncol. 2016;11:61. https://doi.org/10.1186/s13014-016-0636-4
Berschneider B, Ellwanger DC, Baarsma HA, Thiel C, Shimbori C, White ES, Kolb M, Neth P, Königshoff M. miR-92a regulates TGF-β1-induced WISP1 expression in pulmonary fibrosis. Int J Biochem Cell Biol. 2014;53:432-41. https://doi.org/10.1016/j.biocel.2014.06.011
Yang S, Banerjee S, de Freitas A, Sanders YY, Ding Q, Matalon S, Thannickal VJ, Abraham E, Liu G. Participation of miR-200 in pulmonary fibrosis. Am J Pathol. 2012;180(2):484-93. https://doi.org/10.1016/j.ajpath.2011.10.005
He S, Li L, Sun S, Zeng Z, Lu J, Xie L. A novel murine chronic obstructive pulmonary disease model and the pathogenic role of microRNA-21. Front Physiol. 2018;9:503. https://doi.org/10.3389/fphys.2018.00503
Serban KA, Rezania S, Petrusca DN, Poirier C, Cao D, Justice MJ, Patel M, Tsvetkova I, Kamocki K, Mikosz A, Schweitzer KS, Jacobson S, Cardoso A, Carlesso N, Hubbard WC, Kechris K, Dragnea B, Berdyshev EV, McClintock J, Petrache I. Structural and functional characterization of endothelial microparticles released by cigarette smoke. Sci Rep. 2016;6:31596. https://doi.org/10.1038/srep31596
Fujita Y, Araya J, Ito S, Kobayashi K, Kosaka N, Yoshioka Y, Kadota T, Hara H, Kuwano K, Ochiya T. Suppression of autophagy by extracellular vesicles promotes myofibroblast differentiation in COPD pathogenesis. J Extracell Vesicles. 2015;4:28388. https://doi.org/10.3402/jev.v4.28388
Mizuno S, Bogaard HJ, Gomez-Arroyo J, Alhussaini A, Kraskauskas D, Cool CD, Voelkel NF. MicroRNA-199a-5p is associated with hypoxia-inducible factor-1α expression in lungs from patients with COPD. Chest. 2012;142(3):663-672. https://doi.org/10.1378/chest.11-2746
Akbas F, Coskunpinar E, Aynaci E, Oltulu YM, Yildiz P. Analysis of serum micro-RNAs as potential biomarker in chronic obstructive pulmonary disease. Exp Lung Res. 2012;38(6):286-94. https://doi.org/10.3109/01902148.2012.689088
Chen BB, Li ZH, Gao S. Circulating miR-146a/b correlates with inflammatory cytokines in COPD and could predict the risk of acute exacerbation COPD. Medicine (Baltimore). 2018;97(7):e9820. https://doi.org/10.1097/MD.0000000000009820
Mortaz E, Folkerts G, Nijkamp FP, Henricks PA. ATP and the pathogenesis of COPD. Eur J Pharmacol. 2010;638(1-3):1-4. https://doi.org/10.1016/j.ejphar.2010.04.019
Yang L, Chen Z, Xiong W, Ren H, Zhai E, Xu K, Yang H, Zhang Z, Ding L, He Y, Song X, Liu J. High expression of SLC17A9 correlates with poor prognosis in colorectal cancer. Hum Pathol. 2019;84:62-70. https://doi.org/10.1016/j.humpath.2018.09.002
Cui H, Li L, Wang W, Shen J, Yue Z, Zheng X, Zuo X, Liang B, Gao M, Fan X, Yin X, Shen C, Yang C, Zhang C, Zhang X, Sheng Y, Gao J, Zhu Z, Lin D, Zhang A, Wang Z, Liu S, Sun L, Yang S, Cui Y, Zhang X. Exome sequencing identifies SLC17A9 pathogenic gene in two Chinese pedigrees with disseminated superficial actinic porokeratosis. J Med Genet. 2014;51(10):699-704. https://doi.org/10.1136/jmedgenet-2014-102486
Miras-Portugal MT, Menéndez-Méndez A, Gómez-Villafuertes R, Ortega F, Delicado EG, Pérez-Sen R, Gualix J. Physiopathological role of the vesicular nucleotide transporter (VNUT) in the central nervous system: relevance of the vesicular nucleotide release as a potential therapeutic target. Front Cell Neurosci. 2019;13:224. https://doi.org/10.3389/fncel.2019.00224
Larsson M, Sawada K, Morland C, Hiasa M, Ormel L, Moriyama Y, Gundersen V. Functional and anatomical identification of a vesicular transporter mediating neuronal ATP release. Cereb Cortex. 2012;22(5):1203-14. https://doi.org/10.1093/cercor/bhr203
Takai E, Tsukimoto M, Harada H, Sawada K, Moriyama Y, Kojima S. Autocrine regulation of TGF-β1-induced cell migration by exocytosis of ATP and activation of P2 receptors in human lung cancer cells. J Cell Sci. 2012 Nov 1;125(Pt 21):5051-60. https://doi.org/10.1242/jcs.104976
Hanaoka M, Droma Y, Chen Y, Agatsuma T, Kitaguchi Y, Voelkel NF, Kubo K. Carbocisteine protects against emphysema induced by cigarette smoke extract in rats. Chest. 2011;139(5):1101-8. https://doi.org/10.1378/chest.10-0920
Kubysheva N, Boldina M, Eliseeva T, Soodaeva S, Klimanov I, Khaletskaya A, Bayrasheva V, Solovyev V, Villa-Vargas LA, Ramírez-Salinas MA, Salinas-Rosales M, Ovsyannikov DY, Batyrshin I. Relationship of serum levels of IL-17, IL-18, TNF-α, and lung function parameters in patients with COPD, asthma-COPD overlap, and bronchial asthma. Mediators Inflamm. 2020;2020:4652898. https://doi.org/10.1155/2020/4652898
Liang T, Guo L, Liu C. Genome-wide analysis of mir-548 gene family reveals evolutionary and functional implications. J Biomed Biotechnol. 2012;2012:679563. https://doi.org/10.1155/2012/679563
Ke H, Zhao L, Feng X, Xu H, Zou L, Yang Q, Su X, Peng L, Jiao B. NEAT1 is Required for Survival of Breast Cancer Cells Through FUS and miR-548. Gene Regul Syst Bio. 2016;10(Suppl 1):11-7. https://doi.org/10.4137/GRSB.S29414
Saffari M, Ghaderian SMH, Omrani MD, Afsharpad M, Shankaie K, Samadaian N. The association of miR-let 7b and miR-548 with PTEN in prostate cancer. Urol J. 2019;16(3):267-73. https://doi.org/10.22037/uj.v0i0.4564
Zhao G, Wang T, Huang QK, Pu M, Sun W, Zhang ZC, Ling R, Tao KS. MicroRNA-548a-5p promotes proliferation and inhibits apoptosis in hepatocellular carcinoma cells by targeting Tg737. World J Gastroenterol. 2016;22(23):5364-73. https://doi.org/10.3748/wjg.v22.i23.5364
Wang Z, Wu X, Hou X, Zhao W, Yang C, Wan W, Chen L. miR-548b-3p functions as a tumor suppressor in lung cancer. Lasers Med Sci. 2020 Jun;35(4):833-839. https://doi.org/10.1007/s10103-019-02865-7
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