Differential regulation of GS-GOGAT gene expression by plant growth regulators in Arabidopsis seedlings

Authors

  • Milan Dragićević Institute for Biological Research “Siniša Stanković”, Department for Plant Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade
  • Ana Simonović Institute for Biological Research “Siniša Stanković”, Department for Plant Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade
  • Milica Bogdanović Institute for Biological Research “Siniša Stanković”, Department for Plant Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade
  • Angelina Subotić Institute for Biological Research “Siniša Stanković”, Department for Plant Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade
  • Nabil Ghalawenji Institute for Biological Research “Siniša Stanković”, Department for Plant Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade
  • Ivana Dragićević Faculty of Biology, University of Belgrade, Studentski Trg 16, 11000 Belgrade
  • Slađana Todorović Institute for Biological Research “Siniša Stanković”, Department for Plant Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade

Abstract

Primary and secondary ammonium assimilation is catalyzed by the glutamine synthetase-glutamate synthase (GS-GOGAT) pathway in plants. The Arabidopsis genome contains five cytosolic GS1 genes (GLN1;1 – GLN1;5), one nuclear gene for chloroplastic GS2 isoform (GLN2), two Fd-GOGAT genes (GLU1 and GLU2) and a GLT1 gene coding for NADH-GOGAT. Even though the regulation of GS and GOGAT isoforms has been extensively studied in response to various environmental and metabolic cues in many plant species, little is known about the effects of phytohormones on their regulation. The objective of this study was to investigate the impact of representative plant growth regulators, kinetin (KIN), abscisic acid (ABA), gibberellic acid (GA3) and 2,4-dichlorophenoxyacetic acid (2,4-D), on the expression of A. thaliana GS and GOGAT genes. The obtained results indicate that GS and GOGAT genes are differentially regulated by growth regulators in shoots and roots. KIN and 2,4-D repressed GS and GOGAT expression in roots, with little effect on transcript levels in shoots. KIN affected all tested genes; 2,4-D was apparently more selective and less potent. ABA induced the expression of GLN1;1 and GLU2 in whole seedlings, while GA3 enhanced the expression of all tested genes in shoots, except GLU2. The observed expression patterns are discussed in relation to physiological roles of investigated plant growth regulators and N-assimilating enzymes.

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Published

2016-06-27

How to Cite

1.
Dragićević M, Simonović A, Bogdanović M, Subotić A, Ghalawenji N, Dragićević I, Todorović S. Differential regulation of GS-GOGAT gene expression by plant growth regulators in Arabidopsis seedlings. Arch Biol Sci [Internet]. 2016Jun.27 [cited 2024Dec.22];68(2):399-404. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/782

Issue

Vol. 68 No. 2 (2016)

Section

Articles

License

Authors grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.

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