Ascochyta blight (Ascochyta fabae) of faba bean (Vicia faba L.): phenotypic and molecular characterization, pathogenicity and in vitro biological control by Bacillus spp. and Pseudomonas spp.

Authors

  • Bouchra Oguiba Laboratory of Applied Microbiology, Department of Biology, Faculty of Natural and Life Sciences, University of Oran 1 Ahmed Ben Bella, BP1524, El M’naouer-31000 Oran, Algeria https://orcid.org/0000-0001-7252-3183
  • Noureddine Karkachi Laboratory of Applied Microbiology, Department of Biology, Faculty of Natural and Life Sciences, University of Oran 1 Ahmed Ben Bella, BP1524, El M’naouer-31000 Oran, Algeria https://orcid.org/0000-0002-3162-5279
  • Francisca Suárez-Estrella Department of Biology and Geology, University of Almería, Agrifood Campus of International Excellence (ceiA3), Center for Research in Mediterranean Intensive Agrosystems and Agri-Food Biotechnology (CIAIMBITAL), 04120 Almería, Spain https://orcid.org/0000-0003-2549-947X
  • Sadika Haouhach Department of Biotechnology, University of Science and Technology of Oran Mohamed Boudiaf, 31000 Oran, Algeria https://orcid.org/0000-0001-6419-0737
  • Mebrouk Kihal Laboratory of Applied Microbiology, Department of Biology, Faculty of Natural and Life Sciences, University of Oran 1 Ahmed Ben Bella, BP1524, El M’naouer-31000 Oran, Algeria https://orcid.org/0000-0003-2901-373X
  • María J. López Department of Biology and Geology, University of Almería, Agrifood Campus of International Excellence (ceiA3), Center for Research in Mediterranean Intensive Agrosystems and Agri-Food Biotechnology (CIAIMBITAL), 04120 Almería, Spain https://orcid.org/0000-0002-3153-3227

DOI:

https://doi.org/10.2298/ABS230203009O

Keywords:

Ascochyta blight, Ascochyta fabae, biological control, faba bean, pathogenicity, Vicia faba L.

Abstract

Paper description:

  • Ascochyta blight (Ascochyta fabae) is a major biotic constraint for faba bean (Vicia faba ) production worldwide. No information is available in the literature on local isolates from Algeria, and no studies on its biological control exist.
  • 14 strains of fabae were isolated and identified by morpho-molecular characterization and the pathogenicity assay. Isolates were separated into three groups according to their level of aggressiveness.
  • Bacillus siamensis B8 and Bacillus mojavensis B31 were the most effective against all isolates. fabae aggressiveness was negatively correlated with susceptibility to biocontrol strains.
  • These findings have important implications for epidemiological studies and disease management.

Abstract: Ascochyta blight is a major biotic constraint of faba bean (Vicia faba L.) production and productivity worldwide caused by Ascochyta fabae. No studies have been performed in Algeria to identify A. fabae isolates or to assess their pathogenicity, and therefore information on local isolates is insufficient. Herein, 14 isolates of A. fabae were obtained from faba bean samples and identified based on morphological traits and phylogenetic analysis of internal transcribed spacer sequences. All generated sequences have been deposited in GenBank and assigned accession numbers. Pathogenicity tests on faba bean plants revealed that all isolates produced necrotic lesions on aerial parts with variable intensity, classifying them as weakly, moderately, and highly aggressive. The in vitro antifungal activity of Bacillus and Pseudomonas strains against A. fabae isolates showed that Bacillus siamensis B8 and Bacillus mojavensis B31 had the highest suppressive potential against all isolates. Moreover, a negative correlation was found between the aggressiveness of A. fabae isolates and their susceptibility to biocontrol strains. This is the first report on the identification, pathogenicity and in vitro biological control of A. fabae isolates in Algeria. B8 and B31 could be further developed as promising biocontrol agents for the control of the ascochyta blight of faba bean.

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2023-04-03

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Oguiba B, Karkachi N, Suárez-Estrella F, Haouhach S, Kihal M, López MJ. Ascochyta blight (Ascochyta fabae) of faba bean (Vicia faba L.): phenotypic and molecular characterization, pathogenicity and in vitro biological control by Bacillus spp. and Pseudomonas spp. Arch Biol Sci [Internet]. 2023Apr.3 [cited 2024Dec.22];75(1):103-17. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/8375

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Vol. 75 No. 1 (2023)

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Copyright (c) 2023 Bouchra OGUIBA, Noureddine KARKACHI, Francisca SUÁREZ-ESTRELLA , Sadika HAOUHACH, Mebrouk KIHAL, María J. LÓPEZ

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