Seed priming and exogenous application of citric acid enhance seedling growth and photosynthetic pigments and mitigate oxidative damage of soybean (Glycine max) under salt stress

Authors

  • Shahin Imran 1. Department of Agronomy, Khulna Agricultural University, Khulna-9100, Bangladesh; 2. Institute of Plant Science and Resources, Okayama University, 2-20-1, Chuo, Kurashiki 710-0046, Japan https://orcid.org/0000-0002-7423-9984
  • Md. Asif Mahamud Department of Agricultural Chemistry, Khulna Agricultural University, Khulna-9100, Bangladesh
  • Newton Chandra Paul Department of Agronomy, Khulna Agricultural University, Khulna-9100, Bangladesh
  • Jotirmoy Chakrobortty Department of Soil Science, Khulna Agricultural University, Khulna-9100, Bangladesh
  • Prosenjit Sarker Department of Crop Botany, Khulna Agricultural University, Khulna-9100, Bangladesh
  • Shipan Paul Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Md. Tahjib-Ul-Arif Department of Biochemistry and Molecular Biology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Mohammad Saidur Rhaman 1. Department of Seed Science and Technology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh; 2. Peking University Institute of Advanced Agricultural Science, Shandong Laboratory of Advanced Agricultural Science at Weifang, Weifang 261000, Shandong, China

DOI:

https://doi.org/10.2298/ABS230804033I

Keywords:

Citric acid, germination, photosynthetic pigments, seed vigor index, salt stress

Abstract

Paper description:

  • Soybean is an important crop whose seed germination, seedling growth, and physiological and biochemical traits are hampered by salinity.
  • Petri dish and hydroponic pot experiments were conducted using BARI Soybean-6 variety to examine the salinity stress mitigating effect of citric acid.
  • Citric acid increased germination, growth, and photosynthetic pigments, and decreased H2O2 and malondialdehyde (MDA) contents caused by salinity stress.
  • The results of this study may be useful in reducing salinity stress-induced growth inhibition of different crops like soybeans on saline areas in coastal belts and maximizing their potential as agricultural lands.

Abstract: Seed priming and citric acid (CA) supplementation on germination and seedling growth of soybeans were investigated. Soybean seeds were primed with distilled water (control), 1 mM CA (CA1), or 2 mM CA (CA2) and then placed for germination in Petri dishes containing distilled water or 150 mM NaCl (SS), alone or in combination with 1 mM or 2 mM CA. Germinated seeds were placed in hydroponic pots using a similar treatment regimen to that specified for the Petri dishes to obtain seedling growth and biochemical parameters. Salt stress significantly lowered germination, growth traits, relative water content (RWC), and photosynthetic pigment. When soybean seeds were primed with CA under salt stress, the germination rate, final germination percentage, seed vigor index, and number of lateral roots significantly increased. Moreover, supplementation of CA significantly increased fresh and dry shoot and root weight, plant height, RWC, and photosynthetic pigments compared to salt-treated plants. The results also displayed that salt stress considerably increased hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents compared to control plants. Spraying of CA1 and CA2 significantly lowered the levels of H2O2 and MDA in salt-treated plants. Both hierarchical clustering and PCA revealed that the effects of salt stress and CA on germination, growth characteristics, photosynthetic pigments, H2O2, and MDA concentrations strongly interacted with one another. According to the findings, CA could be applied as a seed priming and exogenous agent to help soybeans grow more quickly when exposed to salt stress.

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References

Isayenkov SV, Maathuis FJ. Plant salinity stress: many unanswered questions remain. Front Plant Sci. 2019;10:80. https://doi.org/10.3389/fpls.2019.00080

Rhaman MS, Imran S, Karim MM, Chakrobortty J, Mahamud MA, Sarker P, Tahjib-Ul-Arif M, Robin AH, Ye W, Murata Y, Hasanuzzaman M. 5-aminolevulinic acid-mediated plant adaptive responses to abiotic stress. Plant Cell Rep. 2021;40:1451-69. https://doi.org/10.1007/s00299-021-02690-9

Rhaman MS, Kibria MG, Hoque A. Climate change and its adverse impacts on plant growth in South Asia: current status and upcoming challenges. Phyton. 2022;91(4):695-711. https://doi.org/10.32604/phyton.2022.018898

Rhaman MS, Tania SS, Imran S, Rauf F, Kibria MG, Ye W, Hasanuzzaman M, Murata Y. Seed priming with nanoparticles: An emerging technique for improving plant growth, development, and abiotic stress tolerance. J Soil Sci Plant Nutr. 2022;22(4):4047-62. https://doi.org/10.1007/s42729-022-01007-3

Maggio A, De Pascale S, Ruggiero C, Barbieri G. Physiological response of field-grown cabbage to salinity and drought stress. Eur J Agron. 2005;23(1):57-67. https://doi.org/10.1016/j.eja.2004.09.004

Rasheed F, Anjum NA, Masood A, Sofo A, Khan NA. The key roles of salicylic acid and sulfur in plant salinity stress tolerance. J Plant Growth Regul. 2020;30:1-4. https://doi.org/10.1007/s00344-020-10257-3

Shilev S. Plant-growth-promoting bacteria mitigating soil salinity stress in plants. Appl Sci. 2020;19;10(20):7326. https://doi.org/10.3390/app10207326

Iqbal MN, Rasheed R, Ashraf MY, Ashraf MA, Hussain I. Exogenously applied zinc and copper mitigate salinity effect in maize (Zea mays L.) by improving key physiological and biochemical attributes. Environ Sci Pollut Res. 2018;25:23883-96. https://doi.org/10.1007/s11356-018-2383-6

Betzen BM, Smart CM, Maricle KL, MariCle BR. Effects of increasing salinity on photosynthesis and plant water potential in Kansas salt marsh species. Trans Kans Acad Sci. 2019;122(1-2):49-58. https://doi.org/10.1660/062.122.0105

Cruz JL, Coelho EF, Coelho Filho MA, Santos AA. Salinity reduces nutrients absorption and efficiency of their utilization in cassava plants. Cienc Rural. 2018;48. https://doi.org/10.1590/0103-8478cr20180351

Petretto GL, Urgeghe PP, Massa D, Melito S. Effect of salinity (NaCl) on plant growth, nutrient content, and glucosinolate hydrolysis products trends in rocket genotypes. Plant Physiol Biochem. 2019;141:30-9. https://doi.org/10.1016/j.plaphy.2019.05.012

Nadeem M, Li J, Yahya M, Wang M, Ali A, Cheng A, Wang X, Ma C. Grain legumes and fear of salt stress: Focus on mechanisms and management strategies. Int J Mol Sci. 2019;20;799. https://doi.org/10.3390/ijms20040799

Miransari M. Abiotic and biotic stresses in soybean production. 1st ed. Pagano MC, Miransari M; 2016. The importance of soybean production worldwide; p. 1-26. Academic Press. https://doi.org/10.1016/B978-0-12-801536-0.00001-3

Sabagh AE, Hossain A, Islam MS, Barutçular C, Ratnasekera D, Kumar N, Meena RS, Gharib HS, Saneoka H, da Silva JA. Sustainable soybean production and abiotic stress management in saline environments: A critical review. Aust J Crop Sci. 2019;13(2):228-36. https://doi.org/10.21475/AJCS.19.13.02.P1285

Limba V, Dhillon BS, Singh H. Effect of seed priming and urea foliar application on the performance of soybean (Glycine max L. Merrill). J Pharmacogn Phytochem. 2020;9(6):1270-4.

Bustingorri C, Lavado RS. Soybean growth under stable versus peak salinity. Sci Agric. 2011;68:102-8. https://doi.org/10.1590/S0103-90162011000100015

Borges AA, Jiménez-Arias D, Expósito-Rodríguez M, Sandalio LM, Pérez JA. Priming crops against biotic and abiotic stresses: MSB as a tool for studying mechanisms. Front Plant Sci. 2014;5:642. https://doi.org/10.3389/fpls.2014.00642

El-Hawary MM, Nashed ME. Effect of foliar application by some antioxidants on growth and productivity of maize under saline soil conditions. J Plant Prod Sci. 2019;10(2):93-9. http://dx.doi.org/10.21608/jpp.2019.36238

Chakrobortty J, Imran S, Mahamud MA, Sarker P, Paul NC. Effect of citric acid (CA) priming and exogenous application on germination and early seedling growth of okra (Abelmoschus esculentus L.) plants under salinity stress condition. Arch Agric Environ Sci. 2022;7(3):318-26. https://doi.org/10.26832/24566632.2022.070303

Rhaman MS, Imran S, Rauf F, Khatun M, Baskin CC, Murata Y, Hasanuzzaman M. Seed priming with phytohormones: An effective approach for the mitigation of abiotic stress. Plants. 2020;10(1):37. https://doi.org/10.3390%2Fplants10010037

Soliman M, Elkelish A, Souad T, Alhaithloul H, Farooq M. Brassinosteroid seed priming with nitrogen supplementation improves salt tolerance in soybean. Physiol Mol Biol Plants. 2020;26;501-11. https://doi.org/10.1007/s12298-020-00765-7

Sheteiwy MS, Shao H, Qi W, Daly P, Sharma A, Shaghaleh H, Hamoud YA, El‐Esawi MA, Pan R, Wan Q, Lu H. Seed priming and foliar application with jasmonic acid enhance salinity stress tolerance of soybean (Glycine max L.) seedlings. J Sci Food Agric. 2021;101(5);2027-41. https://doi.org/10.1002/jsfa.10822

Tahjib-Ul-Arif M, Zahan MI, Karim MM, Imran S, Hunter CT, Islam MS, Mia MA, Hannan MA, Rhaman MS, Hossain MA, Brestic M. Citric acid-mediated abiotic stress tolerance in plants. Int J Mol Sci. 2021;22(13):7235. https://doi.org/10.3390/ijms22137235

Zanotti RF, Lopes JC, Motta LB, de Freitas AR, Mengarda LH. Tolerance induction to saline stress in papaya seeds treated with potassium nitrate and sildenafil citrate. Semin Cienc Agrar. 2013;1(34):3669-73. http://dx.doi.org/10.5433/1679-0359.2013v34n6Supl1p3669

Yadav RK, Saini PK, Pratap M, Tripathi SK. Techniques of seed priming in field crops. Int J Chem Stud. 2018;6(3):1588-94.

El-Beltagi HS, Ahmed SH, Namich AA, Abdel-Sattar RR. Effect of salicylic acid and potassium citrate on cotton plant under salt stress. Fresen Environ Bull. 2017;26(1A):1091-100.

Sun YL, Hong SK. Effects of citric acid as an important component of the responses to saline and alkaline stress in the halophyte Leymus chinensis (Trin.). Plant Growth Regul. 2011;64:129-39. http://dx.doi.org/10.1007/s10725-010-9547-9

Ahmed S, Abdel-Razek M, Hafez W, Aziz AE. Environmental impacts of some organic extracts on sugar beet yield under saline-sodic soil conditions. J Soil Sci Agric Eng. 2017;8(12):821-7. https://dx.doi.org/10.21608/jssae.2017.38398

Abdellatif YM, Ibrahim MT. Non-enzymatic antioxidants potential in enhancing Hibiscus sabdariffa L. tolerance to oxidative stress. Int J Botany. 2018;14(1):43-58. https://doi.org/10.3923/ijb.2018.43.58

Ahmed AM, Talaat IM, Khalid KA. Citric acid affects Melissa officinalis L. essential oil under saline soil. Asian J Crop Sci. 2017;9(2):40-9. https://doi.org/10.3923/ajcs.2017.40.49

Noor J, Ullah A, Saleem MH, Tariq A, Ullah S, Waheed A, Okla MK, Al-Hashimi A, Chen Y, Ahmed Z, Ahmed I. 2022. Effect of Jasmonic Acid Foliar Spray on the Morpho-Physiological Mechanism of Salt Stress Tolerance in Two Soybean Varieties (Glycine max L.). Plants. 2022;11(5):651. https://doi.org/10.3390/plants11050651

Mangena P. Role of benzyladenine seed priming on growth and physiological and biochemical response of soybean plants grown under high salinity stress condition. Int J Agron. 2020;2020:8847098. https://doi.org/10.1155/2020/8847098

Alharbi BM, Elhakem AH, Alnusairi GS, Soliman MH, Hakeem KR, Hasan MM, Abdelhamid MT. Exogenous application of melatonin alleviates salt stress-induced decline in growth and photosynthesis in Glycine max (L.) seedlings by improving mineral uptake, antioxidant and glyoxalase system. Plant Soil Environ. 2021;67(4);208-20. https://doi.org/10.17221/659/2020-PSE

Ren H, Wang X, Zhang F, Zhao K, Liu X, Yuan R, Zhou C, Yu J, Du J, Zhang B, Wang J. Salicylic acid and pyraclostrobin can mitigate salinity stress and improve anti-oxidative enzyme activities, photosynthesis, and soybean production under saline–alkali regions. Land. 2023;12(7);1319. https://doi.org/10.3390/land12071319

Malekzadeh P. Influence of exogenous application of glycinebetaine on antioxidative system and growth of salt-stressed soybean seedlings (Glycine max L.). Physiol Mol Biol Plants. 2015;21;225-32. https://doi.org/10.1007/s12298-015-0292-4

Mostofa MG, Fujita M. Salicylic acid alleviates copper toxicity in rice (Oryza sativa L.) seedlings by up-regulating antioxidative and glyoxalase systems. Ecotoxicology. 2013;22:959-73. https://doi.org/10.1007/s10646-013-1073-x

Lichtenthaler HK. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymology. 1987;148;350-82. https://doi.org/10.1016/0076-6879(87)48036-1

Zhang Z, Huang R. Analysis of malondialdehyde, chlorophyll proline, soluble sugar, and glutathione content in Arabidopsis seedling. Bio-protoc. 2013;3(14):e817. https://doi.org/10.21769/BioProtoc.817

Alexieva V, Sergiev I, Mapelli S, Karanov E. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant Cell Environ. 2001;24(12):1337-44. https://doi.org/10.1046/j.1365-3040.2001.00778.x

Shaddad MA. Salt tolerance of crop plants. J Stress Physiol Biochem. 2010;6(3):64-90.

Akram S, Siddiqui MN, Hussain BN, Al Bari MA, Mostofa MG, Hossain MA, Tran LSP. Exogenous glutathione modulates salinity tolerance of soybean [Glycine max (L.) Merrill] at reproductive stage. J Plant Growth Regul. 2017 36;877-88. https://doi.org/10.1007/s00344-017-9691-9

Zhao S, Zhang Q, Liu M, Zhou H, Ma C, Wang P. Regulation of plant responses to salt stress. Int J Mol Sci. 2021;22(9):4609. https://doi.org/10.3390/ijms22094609

Ahanger MA, Agarwal RM. Salinity stress induced alterations in antioxidant metabolism and nitrogen assimilation in wheat (Triticum aestivum L) as influenced by potassium supplementation. Plant Physiol Biochem. 2017;115:449-60. https://doi.org/10.1016/j.plaphy.2017.04.017

Tania SS, Rhaman MS, Rauf F, Rahaman MM, Kabir MH, Hoque MA, Murata Y. Alleviation of Salt-Inhibited germination and seedling growth of kidney bean by seed priming and exogenous application of salicylic acid (SA) and hydrogen peroxide (H2O2). Seeds. 2022;1(2):87-98. https://doi.org/10.3390/seeds1020008

Li Y, Li H, Li Y, Zhang S. Improving water-use efficiency by decreasing stomatal conductance and transpiration rate to maintain higher ear photosynthetic rate in drought-resistant wheat. Crop J. 2017;5(3):231-39. https://doi.org/10.1016/j.cj.2017.01.001

Parvin K, Hasanuzzaman M, Bhuyan MB, Nahar K, Mohsin SM, Fujita M. Comparative physiological and biochemical changes in tomato (Solanum lycopersicum L.) under salt stress and recovery: role of antioxidant defense and glyoxalase systems. Antioxidants. 2019;8(9):350. https://doi.org/10.3390/antiox8090350

Xiu JI, Haoting CH, Yu SH, Longqiang BA, Leiping HO, Yi ZH. Effect of citric acid seed priming on the growth and physiological characteristics of tomato seedlings under low phosphorus stress. Chin J Eco-Agric. 2021;29(7):1159-70. http://dx.doi.org/10.13930/j.cnki.cjea.200953

Hamayun M, Khan SA, Khan AL, Shin JH, Ahmad B, Shin DH, Lee IJ. Exogenous gibberellic acid reprograms soybean to higher growth and salt stress tolerance. J Agric Food Chem. 2010 58(12);7226-32. https://doi.org/10.1021/jf101221t

Marschner H, editor. Marschner’s mineral nutrition of higher plants. Academic press; 2011. https://doi.org/10.1016/C2009-0-63043-9

Nahar K, Rhaman MS, Parvin K, Bardhan K, Marques DN, García-Caparrós P, Hasanuzzaman M. Arsenic-induced oxidative stress and antioxidant defense in plants. Stresses. 2022;2(2):179-209. https://doi.org/10.3390/stresses2020013

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Published

2023-12-13

How to Cite

1.
Imran S, Mahamud MA, Paul NC, Chakrobortty J, Sarker P, Paul S, Tahjib-Ul-Arif M, Rhaman MS. Seed priming and exogenous application of citric acid enhance seedling growth and photosynthetic pigments and mitigate oxidative damage of soybean (Glycine max) under salt stress. Arch Biol Sci [Internet]. 2023Dec.13 [cited 2024Apr.22];75(4):407-18. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/8958

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