Microbial enzymatic activities in soils of Vojvodina, Serbia: insights into the relationship with chemical soil properties
DOI:
https://doi.org/10.2298/ABS231025043KKeywords:
microbial enzymes, soil chemical properties, agricultural soil, soil quality, land useAbstract
Paper description:
- As a significant source of biodiversity, soil is under immense pressure due to indiscriminate exploitation. Microorganisms, a dominant biogenic component of the pedosphere, promptly respond to changes in the soil, which makes them excellent indicators of its quality. Appropriate microbial enzyme synthesis is usually the first response of the microbial community to changes in the environment.
- The relationship between microbial enzymes and the chemical properties of dominant soil types in Vojvodina, Serbia were examined.
- Results reveal correlations between specific enzymes and soil chemical properties.
- Findings point to the possibility of the application of enzymatic activities in agricultural soil quality monitoring.
Abstract: For an agricultural region such as the Vojvodina Province in northern Serbia, soil quality monitoring is very important. Enzymatic activities are proposed as good indicators as they respond to even the slightest changes in the soil. This study aimed to analyze the enzymatic activity levels across three different soil types in Vojvodina and to examine their connection to soil chemical properties and land use. All soil types (chernozem, vertisol, solonchak) were sampled at nine locations, each with 3 field plots. The activities of acid and alkaline phosphatase, β-glucosidase, dehydrogenase, and catalase were measured in samples, as well as the selected chemical properties. Results showed differences in enzymatic activity across different soil types and land use. The most active enzymes in vertisol were acid phosphatase and β-glucosidase; in solonchak, it was alkaline phosphatase; in chernozem, it was dehydrogenase. A high correlation between enzymatic activities and certain soil chemical properties (pH reaction, organic matter, organic carbon, total nitrogen) was also observed, underlining the existence of a relationship between different soil components. The highest determined correlation was between acid phosphatase and pH (r=-0.7), alkaline phosphatase and total nitrogen (r=0.7), and organic matter (r=0.72); the obtained correlations were found to be statistically significant.
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References
Kennedy AC, Smith KL. Soil microbial diversity and the sustainability of agricultural soils. Plant Soil. 1995;170:75-86.
Gougoulias C, Clark JM, Shaw LJ. The role of soil microbes in the global carbon cycle: tracking the below-ground microbial processing of plant-derived carbon for manipulating carbon dynamics in agricultural systems. J Sci Food Agric. 2014;94(12):2362-71.
Cardoso EJBN, Vasconcellos RLF, Bini D, Miyauchi MYH, dos Santos CA, Alves PRL, de Paula AM, Nakatani AS, Pereira J de M, Nogueira MA. Soil health: Looking for suitable indicators. What should be considered to assess the effects of use and management on soil health? Sci Agric. 2013;70(4):274-89.
Milić S, Vasin J, Ninkov J, Zeremski T, Brunet B, Sekulić P. Fertility of privately owned plowland used for field crop production in Vojvodina, Serbia. Ratar i Povrt. 2011;48:359-68.
Filip Z. International approach to assessing soil quality by ecologically-related biological parameters. Agric Ecosyst Environ. 2002;88:169-74.
Pulleman M, Creamer R, Hamer U, Helder J, Pelosi C, Pérès G, Rutgers M. Soil biodiversity, biological indicators and soil ecosystem services-an overview of European approaches. Curr Opin Environ Sustain. 2012;4:529-38.
Acosta-Martínez V, Cruz L, Sotomayor-Ramírez D, Pérez-Alegría L. Enzyme activities as affected by soil properties and land use in a tropical watershed. Appl Soil Ecol. 2007;35:35-45.
Yang L, Zhang Y, Li F. Soil Enzyme Activities and Soil Fertility Dynamics. In: Shrivastava A, editor. Advances in Citrus Nutrition. Dordrecht: Springer; 2012. p. 143-56.
Nannipieri P, Grego S, Ceccanti B. Ecological significance of the biological activity in the soil. In: Bollag JM, Stotzky G, editors. Soil Biochemistry. New York: Marcel Dekker; 1990. p. 293-355.
Nayak DR, Babu YJ, Adhya TK. Long-term application of compost influences microbial biomass and enzyme activities in a tropical Aeric Endoaquept planted to rice under flooded conditions. Soil Biol Biochem. 2007;39:1897-906.
Dick RP, Burns RG. A brief history of soil enzymology research. In: Dick RP, editor. Methods of Soil Enzymology. Madison, USA: Soil Science Society of America; 2015. p. 1-34.
Maharjan M, Sanaullah M, Razavi BS, Kuzyakov Y. Effect of land use and management practices on microbial biomass and enzyme activities in subtropical top-and sub-soils. Appl Soil Ecol. 2017;113:22-8.
Eivazi F, Tabatabai MA. Phosphatases in soils. Soil Biol Biochem. 1977;9:167-72.
Utobo EB, Tewari L. Soil enzymes as bioindicators of soil ecosystem status. Appl Ecol Environ Res. 2015;13(1):147-69.
Eivazi F, Tabatabai MA. Glucosidases and galactosidases in soils. Soil Biol Biochem. 1988;20(5):601-6.
Trevors JT. Dehydrogenase activity in soil: A comparison between the INT and TTC assay. Soil Biol Biochem. 1984;16(6):673-4.
Huang L, Hu W, Tao J, Liu Y, Kong Z, Wu L. Soil bacterial community structure and extracellular enzyme activities under different land use types in a long-term reclaimed wetland. J Soils Sediments. 2019;19:2543-57.
Yao X hua, Min H, Lü Z hua, Yuan H ping. Influence of acetamiprid on soil enzymatic activities and respiration. Eur J Soil Biol. 2006;42:120-6.
Trasar-Cepeda C, Gil-Sotres F, Leirós MC. Thermodynamic parameters of enzymes in grassland soils from Galicia, NW Spain. Soil Biol Biochem. 2007;39:311-9.
Živković B, Nejgebauer V, Tanasijević Đ, Miljković N, Stojković L, Drezgić P. Zemljišta Vojvodine. Novi Sad: Institut za poljoprivredna istraživanja; 1972.
Grujić G, Vasin J, Belić M. Soil salinisation in Vojvodina - the Republic of Serbia. Curr Opin Environ Sustain. 2021;50:149-58.
Zeremski T, Tomić N, Milić S, Vasin J, Schaetzl RJ, Milić D, Gavrilov MB, Živanov M, Ninkov J, Marković SB. Saline soils: A potentially significant geoheritage of the Vojvodina Region, Northern Serbia. Sustain. 2021;13.
Nejgebauer V, Živković B, Tanasijević Đ, Miljković N. Pedološka karta Vojvodine R 1:50.000, Institut za poljoprivredna istraživanja, Novi Sad. 1971.
IUSS Working Group WRB. World Reference Base for Soil Resources. International soil classification system for naming soils and creating legends for soil maps. 4th ed. International Union of Soil Sciences (IUSS), Vienna, Austria. 2022.
Bogdanović D, Lazić S, Belić M, Nešić L, Ćirić V, Čabilovski R. Uzorkovanje zemljišta za ispitivanje plodnosti. In: Manojlović M, editor. Uzorkovanje zemljišta i biljaka za agrohemijske i pedološke analize (priručnik). Univerzitet u Novom Sadu, Poljoprivredni fakultet; 2014.
SRPS ISO 11464. Kvalitet zemljišta - Prethodna obrada uzoraka za fizičko-hemijske analize. 2004;
ISO 10390. Soil quality - Determination of pH. 1994.
ISO 10693. Soil quality - Determination of carbonate content - Volumetric method. Netherlands: Springer Science & Business Media; 1995.
ISO 14235. Soil quality - Determination of organic carbon by sulfochromic oxidation. 1998.
AOAC 972.43. Microchemical determination of carbon, hydrogen, and nitrogen, automated method. 2000.
SRPS ISO 10694. Kvalitet zemljišta - Određivanje organskog i ukupnog ugljenika posle suvog sagorevanja (elementarna analiza). 2005.
Egner H, Riehm H, Domingo WR. Untersuchungen über die chemische Bodenanalyse als Grundlage für die Beurteilung des Nahrstoffzustandes der Boden, II: Chemische Extractionsmetoden zu Phosphorund Kaliumbestimmung. K Lantbrukshügskolans Ann. 1960;26:199-215.
Murphy J, Riley JP. A modified single solution method for the determination of phosphate in natural waters. Anal Chim Acta. 1962;27:31-6.
Thalman A. Zur Methodik der Bestimmung der Dehydrogenase Activitat in Boden Mittels Triphenyltetrazoliumhlorid (TTC). Landw Forsch. 1968;21:249-57.
Klein DA, Loh TC, Goulding RL. A rapid procedure to evaluate the dehydrogenase activity of soils low in organic matter. Soil Biol Biochem. 1971;3(4):385-7.
ISO 23753-1. Determination of dehydrogenase activity in soil Part 1: Method using triphenyltetrazolium (TTC). 2011.
García-Delgado C, D’Annibale A, Pesciaroli L, Yunta F, Crognale S, Petruccioli M, Eymar E. Implications of polluted soil biostimulation and bioaugmentation with spent mushroom substrate (Agaricus bisporus) on the microbial community and polycyclic aromatic hydrocarbons biodegradation. Sci Total Environ. 2015;508:20-8.
Tabatabai MA, Bremner JM. Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol Biochem. 1969;1(4):301-7.
Acosta-Martinez V, Cano A, Johnson J. Simultaneous determination of multiple soil enzyme activities for soil health-biogeochemical indices. Appl Soil Ecol. 2018;126:121-8.
Johnson JL, Temple KL. Some variables affecting the measurement of “catalase activity” in soil. Soil Sci Soc Am J. 1964;28(2):207-9.
Roberge MR. Methodology of enzymes determination and extraction. In: Burns RG, editor. Soil Enzymes. New York: Academic Press; 1978. p. 341-73.
RStudio Team. RStudio: integrated development environment fo R [Internet]. Boston: RStudion, PBC; 2021. Available from: http://www.rstudio.com/
Wickham H, François R, Henry L, Müller K, Vaughan D. dplyr: A grammar of data manipulation [Internet]. 2023. Available from: https://github.com/tidyverse/dplyr
Kuhn M, Jackson S, Cimentada J. corrr: Correlations in R [Internet]. 2022. Available from: https://github.com/tidymodels/corrr, https://corrr.tidymodels.org
Wei T, Simko V. R package “corrplot”: visualization of a correlation matrix [Internet]. Trends in Microbiology. 2017. Available from: https://github.com/taiyun/corrplot
Wickham H. ggplot2: Elegant graphics for data analysis [Internet]. 2nd ed. New York: Springer International Publishing; 2016. Available from: https://ggplot2.tidyverse.org
Pavlović P, Kostić N, Karadžić B, Mitrović M. The Soils of Serbia. Hartemink AE, editor. Madison, USA: Department of Soil Science, FD Hole Soils Laboratory, University of Wisconsin-Madison; 2017.
Milić S, Ninkov J, Zeremski T, Latković D, Šeremešić S, Radovanović V, Žarković B. Soil fertility and phosphorus fractions in a calcareous chernozem after a long-term field experiment. Geoderma. 2019;339:9-19.
Manojlović S. Sistem kontrole plodnosti zemljišta i upotrebe đubriva u Vojvodini - od naučnih istraživanja, preko razvojnih istraživanja do funkcionisanja u poljorivrednoj proizvodnji Vojvodine. In: Zbornik radova Pokrajinskog komiteta za nauku I informatiku, knjiga broj 18. 1986. p. 123-7.
Taylor JP, Wilson B, Mills MS, Burns RG. Comparison of microbial numbers and enzymatic activities in surface soils and subsoils using various techniques. Soil Biol Biochem. 2002;34:387-401.
Miljković N. Ameliorative Pedology. University of Novi Sad, Faculty of Agriculture, Water Management Department, Public Water Management Company “Vode Vojvodina”, Novi Sad. 2005.
Acosta-Martínez V, Tabatabai MA. Enzyme activities in a limed agricultural soil. Biol Fertil Soils. 2000;31:85-91.
Turner BL, Haygarth PM. Phosphatase activity in temperate pasture soils: Potential regulation of labile organic phosphorus turnover by phosphodiesterase activity. Sci Total Environ. 2005;344:27-36.
Aon MA, Colaneri AC. II. Temporal and spatial evolution of enzymatic activities and physico-chemical properties in an agricultural soil. Appl Soil Ecol. 2001;18:255-70.
Adetunji AT, Ncube B, Meyer AH, Mulidzi R, Lewu FB. Soil β-glucosidase activity, organic carbon and nutrients in plant tissue in response to cover crop species and management practices. South African J Plant Soil. 2020;37(3):202-10.
Tan X, Xie B, Wang J, He W, Wang X, Wei G. County-scale spatial distribution of soil enzyme activities and enzyme activity indices in agricultural land: Implications for soil quality assessment. Sci World J. 2014;2014.
Fernández-Calviño D, Soler-Rovira P, Polo A, Díaz-Raviña M, Arias-Estévez M, Plaza C. Enzyme activities in vineyard soils long-term treated with copper-based fungicides. Soil Biol Biochem. 2010;42:2119-27.
Moeskops B, Sukristiyonubowo, Buchan D, Sleutel S, Herawaty L, Husen E, Saraswati R, Setyorini D, De Neve S. Soil microbial communities and activities under intensive organic and conventional vegetable farming in West Java, Indonesia. Appl Soil Ecol. 2010;45:112-20.
Kong Y, Qu A, Feng E, Chen R, Yang X, Lai Y. Seasonal Dynamics of Soil Enzymatic Activity under Different Land-Use Types in Rocky Mountainous Region of North China. Forests. 2023;14(3).
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Copyright (c) 2023 Ana Kuzmanović, Dragana Tamindžija, Jordana Ninkov, Jovica Vasin, Simonida Đurić, Stanko Milić, Dragan Radnović
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