Analgesic and antiinflammatory activities of the aqueous root extract of Algerian Bunium incrassatum
DOI:
https://doi.org/10.2298/ABS231101042AKeywords:
Bunium incrassatum, polyphenols, Antioxidant, Analgesic, Anti-inflammatoryAbstract
Paper description:
- Bunium incrassatum, commonly known as Black Cumin, is a plant that has been traditionally used in various cultures for culinary and medicinal purposes.
- The in vitro antioxidant and in vivo analgesic and anti-inflammatory activities of the aqueous extract from the roots of B. incrassatum were examined in mice.
- The aqueous extract of B. incrassatum exhibited in vivo anti-inflammatory activity in xylene- and Croton oil-induced ear edema, carrageenan-induced paw edema, and analgesic activity in acetic acid writhing and formalin paw-licking experimental models.
- B. incrassatum can be an effective source of compounds with antioxidant, anti-inflammatory, and analgesic effects.
Abstract: The present study was designed to evaluate the antioxidant, analgesic, and antiinflammatory activities of Bunium incrassatum aqueous extract (BIAE) from roots. Tests of radical scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and induced erythrocyte hemolysis using 2,2′-azo bis (2-amidinopropane) dihydrochloride (AAPH) were used for antioxidant activity evaluation. The antiinflammatory activity was tested in Croton oil- and xylene-induced ear edema and carrageenan-induced paw edema. The antinociceptive effect was tested with the pain model induced by formalin and acetic acid-induced writhing response. The results revealed that BIAE exhibited a strong protective effect against AAPH-induced hemolysis of erythrocytes. In contrast, in the DPPH test, BIAE showed moderate activity (IC50: 1.07±0.078 mg/mL) compared to BHT. In the antiinflammatory test, oral administration of BIAE (100, 300, and 600 mg/kg) significantly reduced the edema in the three models used. In antinociceptive experiments, the pretreatment with BIAE produced important analgesic activity. Additionally, the pretreatment of mice with BIAE significantly reduced the paw-licking time in the second phase. The results of this study revealed the antioxidant, analgesic, and antiinflammatory potential of BIAE, and demonstrated the importance of B. incrassatum as a source of compounds for therapeutic uses.
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References
McGill MR, Jaeschke H. Oxidant stress, antioxidant defense, and liver injury. In: Kaplowitz N, DeLeve LD, editors. Drug-Induced Liver Dis. Academic Press; 2013. p. 71-84.
https://doi.org/10.1016/B978-0-12-387817-5.00004-2
Nasri M, Bedjou F, Porras D, Martínez-Flórez S. Antioxidant, anti-inflammatory, and analgesic activities of Citrus reticulata Blanco leaves extracts: An in vivo and in vitro study. Phytothérapie. 2018;16(S1):S130-42.
https://doi.org/10.3166/phyto-2019-0141
Majid M, Khan MR, Shah NA, Haq IU, Farooq MA, Ullah S, Sharif A, Zahra Z, Younis T, Sajid M. Studies on phytochemical, antioxidant, anti-inflammatory and analgesic activities of Euphorbia dracunculoides. BMC Complement Altern Med. 2015;15:349.
http://doi: 10.1186/s12906-015-0868-0
Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, Li Y, Wang X, Zhao L. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2018;9(6):7204-18.
https://doi.org/10.18632/oncotarget.23208
Alam B, Akter F, Parvin N, Pia RS, Akter S, Chowdhury J, Sifath-E-Jahan K, Haque E. Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves. Avicenna J Phytomed. 2013;3(2):112-25.
Ghlichloo I. Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) [Internet]. StatPearls; [updated 2023 May 1; cited 2023 Nov 1]. Available from: https://www.statpearls.com/point-of-care/25968
Berroukeche F, Attoui N, Toul F, Ziane M, Soulimane MN, Merzouk H. Investigation of antioxidant and anti-hemolytic properties of Algerian Bunium incrassatum tubers and their effects as diet on histological and biochemical parameters of normal Wistar rats. Asian J Agric Biol. 2022(1):1-11.
https://doi.org/10.35495/ajab.2020.07.391
Taïbi K, Ait Abderrahim L, Helal F, Hadji K. Ethnopharmacological study of herbal remedies used for the management of thyroid disorders in Algeria. Saudi Pharm J. 2021;29:43-52.
https://doi.org/10.1016/j.jsps.2020.12.004
Bousetla A, Zellagui A, Derouiche K, Rhouati S. Chemical constituents of the roots of Algerian Bunium incrassatum and evaluation of its antimicrobial activity. Arab J Chem. 2015;8:313-16.
https://doi.org/10.1016/j.arabjc.2011.01.022
Toul F, Djendar A, Seladji M, Berroukeche F. Algerian Bunium incrassatum seeds: effects of extraction solvent polarity on phenolic profile and antioxidant activity. JOTCSA. 2022;9(2):415-22.
https://doi.org/10.18596/jotcsa.1058060
Hajhashemi V, Sajjadi SE, Zomorodkia M. Antinociceptive and anti-inflammatory activities of Bunium persicum essential oil, hydroalcoholic and polyphenolic extracts in animal models. Pharm Biol. 2011, 49(2):146-51.
https://doi.org/10.3109/13880209.2010.504966
Ndebia EJ, Kamgang R, Nkeh-ChungagAnye NB. Analgesic and anti-inflammatory properties of aqueous extract from leaves of Solanum torvum (Solanaceae) Afr. J. Trad. CAM. 2007;4(2):240-4.
Amraoui N , Laroui H, Charef N, Baghiani A, Arrar L. Antioxidant, anti-hemolytic and anti-inflammatory activities of aqueous extract of aerial parts of Rosmarinus Tournefortii. Annal RSCB. 2022;26(1):2912-23.
Krache I, Boussoualim N, Ouhida S, Amraoui N, Baghiani A, Arrar L. Acute and chronic effects of methanolic extract of Teucrium polium on blood parameters and histopathology of liver and kidney in female rats. AJRIMPS. 2017;2(3):1-11.
https://doi.org/10.9734/AJRIMPS/2017/38234
Amraoui N, Mayouf N, Charef N, Baghiani A, Arrar L. Antioxidant, anti-inflammatory and anti-arthritic activities of methanol extract of Tamus communis L. roots. Trop J Pharm Res. 2019;18(7):1499-506.
https://doi.org/10.4314/tjpr.v18i7.20
Guemmaz T, Zerargui F, Boumerfeg S, Arrar L, Aouachria S, Khennouf S, Charef N, Baghiani A. Anti-hemolytic, anti-lipid peroxidation, antioxidant properties and acute toxicity of Xanthium strumarium leaves extracts. Ann Res Rev Biol. 2018; 24(3):1-12.
https://doi.org/10.9734/ARRB/2018/40024
OECD. Guidelines for the testing of chemicals. Acute oral toxicity: Up and down procedure, 425. 2008.
Cheniti W, Amraoui N, Roumili I, Abdelouhab K, Charef N, Baghiani A, Arrar L. Anti-inflammatory effects of different parts of Algerian caper (Capparis spinosa) on animal models. South Asian J Exp Biol. 2022;12(5): 661-70.
https://doi.org/10.38150/sajeb.12(5).p661-670
Hisamuddin N, Shaik Mossadeq WM, Sulaiman MR, Abas F, Leong SW, Kamarudin N, Ong HM, Ahmad Azmi AF, Ayumi RR, Talib M. Anti-edematogenic and anti-granuloma activity of a synthetic curcuminoid analog, 5-(3,4-Dihydroxyphenyl)-3-hydroxy-1-(2-hydroxyphenyl) penta-2,4-dien-1-one, in mouse models of inflammation. Molecules. 2019; 24(14):2614.
https://doi.org/10.3390/molecules24142614
Yimer T, Birru EM, Adugna M, Geta M, Emiru YK. Evaluation of analgesic and antiinflammatory activities of 80% methanol root extract of Echinops kebericho M. (Asteraceae). J Inflamm Res. 2020;13:647-58.
https://doi.org/10.2147/JIR.S267154.eCollection 2020
Mustaffa F, Indurkar J, Ismail S, Mordi MN, Ramanathan S, Mansor SM. Analgesic activity, toxicity study and phytochemical screening of standardized Cinnomomum iners leaves methanolic extract. Pharmacog Res. 2010; 2(2):76-81.
https://doi.org/10.4103/0974-8490.62952
Dar RA, Shahnawaz M, Ahanger MA, Majid IU. Exploring the diverse bioactive compounds from medicinal plants: A Review. J Phytopharmacol. 2023;12(3):189-95.
https://doi.org/10.31254/phyto.2023.12307
Ivanišová E, Kačániová M, Savitskaya TA, Grinshpan DD. Medicinal herbs: important source of bioactive compounds for food industry. In: Ahmed RS. Editor. Herbs and Spices - New Processing Technologies. London. IntechOpen; 2021.
https://doi.org/10.5772/intechopen.98819
Dehimi K, Djoudi Z, Boulaoud A, Maadadi A, Dahamna S, Khennouf S. A contribution to the valorization of two medicinal plants: Atriplex halimus Sub. Sp. Schwein furthii and Bunium Incrassatum, growing in the region of M’sila (North-East Algeria). Indian J Nov Drug Deliv. 2020;12(4):208-16.
Bouterfas K, Mehdadi Z, Benmansour D, Khaled MB, Bouterfas M, Latreche A. Optimization of extraction conditions of some phenolic compounds from white horehound (Marrubium vulgare L.) Leaves. Inter J Org Chem. 2014;4:292-308.
https://doi.org/10.4236/ijoc.2014.45032
Ruiz-Ruiza JC, Matus-Bastoa AJ, Acereto-Escoffiéb P, Segura-Campos MR. Antioxidant and anti-inflammatory activities of phenolic compounds isolated from Melipona beecheii honey. Food Agric Immunol. 2017;28(6):1424-37.
https://doi.org/10.1080/09540105.2017.1347148
Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, Pandey RP, Chang C-M. Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa. Molecules. 2022; 27(4):1326.
https://doi.org/10.3390/molecules27041326
Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N. Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from western Nepal. Plants. 2019;8(4):96.
https://doi.org/10.3390/plants8040096
Kherbachi S, Kheniche M, Tacherfiout M. Antihemolytic activity of hydroalcoholic leaves and bark extracts from Rhamnus alaternus against AAPH induced hemolysis on human erythrocytes. Int J Plant Bas Pharm. 2022; 2(2):210-19.
https://doi.org/10.29228/ijpbp.3
Ramchoun M, Sellam K, Harnafi H, Alem C, Benlyas M, Khallouki F, Amrani S. Investigation of antioxidant and antihemolytic properties of Thymus satureioides collected from Tafilalet Region, south-east of Morocco. Asian Pac J Trop Biomed. 2015;5(2):93-100.
https://doi.org/10.1016/S2221-1691(15)30151-9
Zhang, J, Wang H, Wang T, Chong Y, Yu P, Lu C, Xue Y, Fu F, Zhang L. Anti-inflammatory activity of Yanshu spraying agent in animal models. Exp Ther Med. 2013; 5(1):73-76.
https://doi.org/10.3892/etm.2012.761
Lee CY, Park SH, Lim HY, Jang SG, Park KJ, Kim DS, Kim JH, Cho JY. In vivo anti-inflammatory effects of Prasiola japonica ethanol extract. J Funct Foods. 2021;80:104440.
https://doi.org/10.1016/j.jff.2021.104440
Khan H, Khan MA, Gul F, Hussain S, Ashraf N. Anti-inflammatory activity of Heliotropium strigosum in animal models. Toxicol Ind Health. 2013;31(12):1281-7.
Singsai K, Charoongchit P, Chaikaew W, Boonma N, Fhanjaksai P and Chaisatan K. Antilipoxygenase and anti-inflammatory activities of Streblus asper leaf extract on xylene-induced ear edema in mice. Adv Pharmacol Pharm Sci. 2020;2020:3176391.
https://doi.org/10.1155/2020/3176391
Foong CP, Hamid RA. Evaluation of anti-inflammatory activities of ethanolic extract of Annona muricata leaves. Rev Bras Farmacogn. 2012;22(6):1301-7.
Okokon JE, Okokon PJ, Dar Farooq A, Choudhary MI. Anti-inflammatory and antinociceptive activities of Homalium letestui. Pharm Biol. 2013;51(11):1459-66.
https://doi.org/10.3109/13880209.2013.799707
Maxia A, Frau MA, Falconieri D, Karchuli MS, Kasture S. Essential oil of Myrtus communis inhibits inflammation in rats by reducing serum IL-6 and TNF–α. Nat Prod Commun. 2011;6(10):1545-8.
Puspawati N M, Rita WS. Topical anti-inflammatory activity of n-hexane extract of santalum albumlinn leaves on rat ear oedema induced by croton oil. J PhysConf Ser. 2019;1321:022033.
https://doi.org/10.1088/1742-6596/1321/2/022033
Li LS, Chiroma SM, Hashim T, Adam SK, MohdMoklas MA, Yusuf Z, Rahman SA. Antioxidant and anti-inflammatory properties of Erythroxylum cuneatum alkaloid leaf extract. Heliyon. 2020;6(6):e04141.
https://doi.org/10.1016/j.heliyon.2020.e04141
Yilmazer N, Coskun C, Gurel-Gurevin E, Yaylim I, Eraltan EH, Ikitimur-Armutak EI. Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edema. Pol J Vet Sci. 2016;19(3):589-95.
https://doi.org/10.1515/pjvs-2016-0074
Zammel N, Saeed M, Bouali N, Elkahoui S, Alam JM, Rebai T, Kausar MA, Adnan M, Siddiqui AJ, Badraoui R. Antioxidant and anti-inflammatory effects of Zingiber officinale roscoe and Allium subhirsutum: in silico, biochemical and histological study. Foods. 2021;10(6):1383.
https://doi.org/10.3390/foods10061383
Park SH, SeongYH. Anti-nociceptive and anti-inflammatory effect of an ethanol extract mixture of Vitisa murensis, Aralia cordata, and Glycyrrhizae radix. J Biomed Transl Res. 2017;18(2):60-6.
https://doi.org/10.12729/jbtr.2017.18.2.060
Zeashan H, Amresh G, Rao CV, Singh S. Antinociceptive activity of Amaranthus spinosus in experimental animals. J Ethnopharmacol. 2009;122(3):492-6.
https://doi.org/10.1016/j.jep.2009.01.031
Karim N, Khan I, Khan W, Khan I, Khan A, Halim SA, Khan H, Hussain J, Al-Harrasi A. Anti-nociceptive and anti-inflammatory activities of Asparacosin A involve selective cyclooxygenase 2 and inflammatory cytokines inhibition: an in-vitro, in-vivo, and in-silico approach. Front Immunol. 2019;10:581.
He X, Yang Y, Yuan X, Sun Y, Li Y. Anti-nociceptive and anti-inflammatory activities of the ethyl acetate extract of Belam candachinensis (L.) redouté in raw 264.7 cells in vitro and mouse model in vivo. J Pain Res. 2022;15:1221-32.
https://doi.org/10.2147/JPR.S353357
Chang CW, Chang WT, Liao JC, Chiu YJ, Hsieh MT, Peng WH, Lin YC. Analgesic and anti-inflammatory activities of methanol extract of Cissus repens in mice. Evid Based Complement Alternat Med. 2012;2012:135379.
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