Comparative 16s metagenomic analysis of prokaryotic diversity in freshwater and permanent snow-line glacial lakes in Türkiye

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DOI:

https://doi.org/10.2298/ABS240324016P

Keywords:

freshwater lakes, glacial lakes, 16s metagenomics, prokaryotic diversity, high altitude ecosystems

Abstract

Paper description:

  • Freshwater lakes support diverse bacterial communities vital for healthy ecosystems. Pollution and climate change can disrupt these communities.
  • This study employed metagenomics to compare the bacterial diversity in three Turkish lakes: Beyşehir, Eber, and Uludağ Buzlu.
  • Distinct bacterial compositions were observed. Beyşehir had the highest diversity with Planktophila and Limnohabitans Eber Lake was characterized by Limnohabitans, Flavobacterium, and Sphingomonas. Uludağ Buzlu Lake had low diversity with Parabacteroides and Akkermansia being the most common.
  • This study provides foundational data on bacterial communities in Turkish lakes, highlighting pollution and altitude effects. This facilitates freshwater ecosystem monitoring and broader environmental change research.

Abstract: Freshwater lakes are critical to healthy ecosystems, providing vital services like drinking water and recreation for surrounding communities. Microorganisms within these ecosystems play essential roles, driving biogeochemical cycles for elements like carbon, nitrogen, and sulfur. This study utilized a metagenomic approach to examine the prokaryotic communities of three freshwater lakes in Türkiye: the Eber and Beyşehir lakes, located at close altitudes (967 m and 1,115 m, respectively), which serve as primary water sources for nearby communities, and Lake Uludag Buzlu (2,390 m) that lies at the permanent snow border within the Uludag glacial lake system. Metagenomics allowed us to identify species, genetic structures, and the functional roles of microorganisms. Employing high-throughput next-generation sequencing (NGS) technology, we analyzed 16S ribosomal DNA (rDNA) sequences (V3-V4 regions) from the lake samples. EzBioCloud software facilitated the analysis of prokaryotic diversity obtained using Illumina NovaSeq technology. While Eber and Beyşehir lakes had similar diversity, Bacillota dominated in the higher-altitude Lake Uludag Buzlu. Genus-level analysis revealed Parabacteroides as the most prevalent in Lake Uludag Buzlu, contrasting with Limnohabitans dominance in Lake Eber; Lake Beyşehir exhibited co-dominance of Limnohabitans and Planktophila.

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Published

2024-07-10

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1.
Pat F, Fidan Pedük S, Akçay N, Kızıl Pat HK, Arıcan E. Comparative 16s metagenomic analysis of prokaryotic diversity in freshwater and permanent snow-line glacial lakes in Türkiye . Arch Biol Sci [Internet]. 2024Jul.10 [cited 2024Oct.30];76(2):233-4. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/9682

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Vol. 76 No. 2 (2024)

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Articles

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Copyright (c) 2024 Fahri Pat, Sultan Fidan Pedük, Neşe Akçay, Hatice Kübra Kızıl Pat, Ercan Arıcan

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