SUBCHRONIC EXPOSURE TO ACRYLAMIDE AFFECTS COLON MUCIN SECRETION IN JUVENILE WISTAR RATS

Authors

  • Ivana Koledin Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad
  • Renata Kovač Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad
  • Vesna Rajković Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad
  • Milica Matavulj Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Novi Sad

Abstract

Acrylamide (AA) is an important industrial chemical worldwide. AA also forms naturally in many high-carbohydrate foods (bread, French fries, coffee, etc.) when they are heated. Since AA is ubiquitous in the human diet, and more than one-third of the calories we take in each day come from foods with detectable levels of acrylamide, the aim of this study was to determine the effect of subchronic AA treatment on colon goblet cell mucin secretion. Male Wistar rats were gavaged with AA for 5 days a week for 21 days. The animals were divided into three groups that were gavaged with different AA concentrations (0, 25, 50 mg/kg/day). Colon samples were processed for histochemical (PAS-AB, HID-AB) and immunohistochemical (anti-rat MUC2 antibody) staining to visualize mucins in the goblet cells. AA treatment showed an alteration in mucin production and secretion in that the amount of all investigated mucin types dropped. More prominent changes were detected in the upper crypt part part where a decreased number of goblet cell was observed. AA treatment elicited a significant reduction in neutral mucins, while acidic mucins showed linearly decreasing trend with respect to AA doses. Also, a linear reduction of MUC2 mucins was noticed. Sulfomucins were absent in the colon lower crypt part in all experimental groups, while in the upper crypt part both sulfo- and sialomucins were significantly decreased. The results of our study point to changes in the synthesis, differentiation and distribution of mucins after AA treatment, which can have adverse effect on colorectal health.

 

Key words: acrylamide; colon; goblet cells; mucins; juvenile rats

 

Received: October 15, 2015; Revised: December 31, 2015; Accepted: January 6, 2016; Published online: June 16, 2016

Downloads

Download data is not yet available.

References

Tareke E, Rydberg P, Karlsson P, Eriksson S, Törnqvist M. Acrylamide: a cooking carcinogen? Chem Res Toxicol. 2000;13(6):517-22.

Smith CJ, Perfetti TA, Rumple MA, Rodgman A, Doolittle DJ. “IARC Group 2A Carcinogens” reported in cigarette mainstream smoke. Food Chem Toxicol. 2000;38(4):371-83.

Dybing E, Farmer PB, Andersen M, Fennell TR, Lalljie SPD, Müller DJG, Olin S, Petersen BJ, Schlatter J, Scholz G, Scimeca JA, Slimani N, Törnqvist M, Tuijtelaars S, Verger P. Human exposure and internal dose assessments of acrylamide in food. Food Chem Toxicol. 2005;43(3):365-410.

International Agency for Research on Cancer (IARC). Acrylamide. In: IARC. IARC monographs on the evaluation of carcinogenic risks to humans. Vol. 60, Some industrial chemicals. Lyon: International Agency for Research on Cancer; 1994. p. 389-433.

LoPachin R, Balaban C, Ross J. Acrylamide axonopathy revisited. Toxicol Appl Pharmacol. 2003;188(3):135-53.

Dearfield KL, Douglas GR, Ehling UH, Moore MM, Sega GA, Brusick DJ. Acrylamide: a review of its genotoxicity and an assessment of heritable genetic risk. Mutat Res Mol Mech Mutagen. 1995;330(1-2):71-99.

Hagmar L, Törnqvist M, Nordander C, Rosén I, Bruze M, Kautiainen A, Magnusson AL, Malmberg B, Aprea P, Granath F, Axmon A, LicMedSc. Health effects of occupational exposure to acrylamide using hemoglobin adducts as biomarkers of internal dose. Scand J Work Environ Health. 2001;27(4):219-26.

Linden SK, Sutton P, Karlsson NG, Korolik V, McGuckin MA. Mucin in the mucosal barrier to infection. Mucosal Immunol. 2008;1(3):183-97.

Iwai T, Ichikawa T, Kida M, Goso Y, Saegusa Z, Okayasu I, Saigenji K, Ishihara K. Vulnerable sites and changes in mucin in the rat small intestine after non-steroidal anti-inflammatory drugs administration. Dig Dis Sci. 2010;55(12):3369-76.

Kim YS, Ho SB. Intestinal goblet cells and mucins in health and disease: recent insights and progress. Curr Gastroent Rep. 2010;12(5):319-30.

Allen A, Hutton DA, Pearson JP. The MUC2 gene product: a human intestinal mucin. Int J Biochem Cell Biol. 1998;30(7):797-801.

Dharmani P, Srivastava V, Kissoon-Singh V, Chadee K. Role of intestinal mucins in innate host defense mechanisms against pathogens. J Innate Immun. 2009;1:123-35.

Kim J, Khan W. Goblet Cells and Mucins: Role in Innate Defense in Enteric Infections. Pathogens. 2013;2(1):55-70.

MacAdam A. The effects of gastro-intestinal mucus on drug absorption. Adv Drug Deliver Rev. 1993;11:201-20.

Deplancke B, Gaskins HR. Microbial modulation of innate defense: Goblet cells and the intestinal mucus layer. Am J Clin Nutr. 2001;73(6):1131S-1341S.

Perez-Vilar J, Mabolo R. Gel-forming mucins. Notions from in vitro studies. Histol Histopathol. 2007;22(4):455-64.

Filipe MI. Mucous secretion in rat colonic mucosa during carcinogenesis induced by dimethylhydrazine. A morphological and histochemical study. Br J Cancer. 1975;32(1):60-77.

Bansil R, Turner BS. Mucin structure, aggregation, physiological functions and biomedical applications. Curr Opin Colloid Interface Sci. 2006;11:164-70.

Schettgen T, Kütting B, Hornig M, Beckmann MW, Weiss T, Drexler H, Angerer J. Trans-placental exposure of neonates to acrylamide-a pilot study. Int Arch Occup Environ Health. 2004;77(3):213-6.

Sörgel F, Weissenbacher R, Kinzig-Schippers M, Hofmann A, Illauer M, Skott A, Landersdorfer C. Acrylamide: increased concentrations in homemade food and first evidence of its variable absorption from food, variable metabolism and placental and breast milk transfer in humans. Chemotherapy. 2002;48(6):267-74.

Fohgelberg P, Rosén J, Hellenäs KE, Abramsson-Zetterberg L. The acrylamide intake via some common baby food for children in Sweden during their first year of life-an improved method for analysis of acrylamide. Food Chem Toxicol. 2005;43(6):951-9.

Schabacker J, Schwend T, Wink M. Reduction of acrylamide uptake by dietary proteins in a Caco-2 gut model. J Agric Food Chem. 2004;52(12):4021-5.

Zödl B, Schmid D, Wassler G, Gundacker C, Leibetseder V, Thalhammer T, Ekmekcioglu C. Intestinal transport and metabolism of acrylamide. Toxicology. 2007;232(1-2):99-108.

El-Bohi KM, Moustafa GG, El Sharkawi NI, Sabik LME. Acrylamide-induced genotoxic, biochemical and pathological perturbations in male rats liver. J Am Sci. 2011;7:1092-6.

Rawi SM, Marie MAS, Fahmy SR, El-Abied SA. Hazardous effects of acrylamide on immature male and female rats. Afr J Pharm Pharmacol. 2012;6:1367-86.

Liévin-Le Moal V, Servin AL. The front line of enteric host defense against unwelcome intrusion of harmful microorganisms: Mucins, antimicrobial peptides, and Microbiota. Clin Microbiol Rev. 2006;19(2):315-37.

McGuckin MA, Eri R, Simms LA, Florin TH, Radford-Smith G. Intestinal barrier dysfunction in inflammatory bowel diseases. Inflamm Bowel Dis. 2009;15(1):100-13.

McCracken BA, Gaskins HR, Ruwe-Kaiser PJ, Klasing KC, Jewell DE. Diet-dependent and diet-independent metabolic responses underlie growth stasis of pigs at weaning. J Nutr. 1995;125(11):2838-45.

Sharma R, Schumacher U, Ronaasen V, Coates M. Rat intestinal mucosal responses to a microbial flora and different diets. Gut. 1995;36(2):209-14.

Satchithanandam S, Vargofcak-Apker M, Calvert RJ, Leeds AR, Cassidy MM. Alteration of gastrointestinal mucin by fiber feeding in rats. J Nutr. 1990;120(10):1179-84.

Greval RK, Mahmood A. Ethanol induced changes in glycosylation of mucins in rat intestine. Annals of gastroenterology. 2009;22(3):178-83.

Johansson MEV. The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria. Gut Microbes. 2010;1(1):51-4.

Johansson MEV, Hansson GC. Mucus and the Goblet cell. Dig Dis. 2013;31(0):305-9.

Van der Sluis M, De Koning BAE, De Bruijn ACJM, Velcich A, Meijerink JPP, Van Goudoever JB, Büller HA, Dekker J, van Seuningen I, Renes IB, Einerhand AWC. Muc2-Deficient Mice Spontaneously Develop Colitis, Indicating That MUC2 Is Critical for Colonic Protection. Gastroenterology. 2006;131(1):117-29.

Velcich A, Yang W, Heyer J, Fragale A, Nicholas C, Viani S, Kucherlapati R, Lipkin M, Yang K, Augenlicht L. Colorectal cancer in mice genetically deficient in the mucin Muc2. Science. 2002;295(5560):1726-9.

Lan A, Andriamihaja M, Blouin JM, Liu X, Descatoire V, de Maredsous CD, Davila AM, Walker F, Tomé D, Blachier F. High-protein diet differently modifies intestinal goblet cell characteristics and mucosal cytokine expression in ileum and colon. J Nutr Biochem. 2015;26(1):91-8.

Dobrowolski P, Huet P, Karlsson P, Eriksson S, Tomaszewska E, Gawron A, Pierzynowski SG. Potato fiber protects the small intestinal wall against the toxic influence of acrylamide. Nutrition. 2012;28(4):428-35.

Tomaszewska E, Dobrowolski P, Puzio I, Prost L, Kurlak P, Sawczuk P, Badzian B, Hulas-Stasiak M, Kostro K. Acrylamide-induced prenatal programming of intestine sructure in guinea pig. J Physiol Pharmacol. 2014;(14):107-15.

El-Mehi AE, El-Sherif NM. Influence of acrylamide on the gastric mucosa of adult albino rats and the possible protective role of rosemary. Tissue Cell. 2015;47(3):273-83.

Rodriguez-Ramiro I, Ramos S, Bravo L, Goya L, Martin MA. Procyanidin B2 and cocoa polyphenolic extract inhibit acrylamide-induced apoptosis in human Caco-2 cells by preventing oxidative stress and activation of JNK pathway. J Nutr Biochem. 2011;22(12):1186-94.

Chen W, Su H, Xu Z, Bao T, Zheng X. Protective effect of wild raspberry (Rubus hirsutus Thunb.) extract against acrylamide-induced oxidative damage is potentiated after simulated gastrointestinal digestion. Food Chem. 2016;196:943-52.

Corfield AP, Myerscough N, Longman R, Sylvester P, Arul S, Pignatelli M. Mucins and mucosal protection in the gastrointestinal tract: new prospects for mucins in the pathology of gastrointestinal disease. Gut. 2000;47(4):589-94.

Corfield AP, Myerscough N, Warren BF, Durdey P, Paraskeva CSR, Schauer R. Reduction of sialic acid O-acetylation in human colonic mucins in the adenoma-carcinoma sequence. Glycoconj J. 1999;16(6):307-17.

Yusuf S, Nok AJ, Ameh DA, Adelaiye AB, Balogun EO. Correlation of gastric mucosal damage with sialic acid profile in rats: effects of hydrochloric acid, pepsin and hypertonic saline. Cell Biochem Funct. 2005;23(5):339-45.

Kovac R, Rajkovic V, Koledin I, Matavulj M. Acrylamide alters glycogen content and enzyme activities in the liver of juvenile rat. Acta Histochem. 2015;117(8):712-17.

Downloads

Published

2016-09-05

How to Cite

1.
Koledin I, Kovač R, Rajković V, Matavulj M. SUBCHRONIC EXPOSURE TO ACRYLAMIDE AFFECTS COLON MUCIN SECRETION IN JUVENILE WISTAR RATS. Arch Biol Sci [Internet]. 2016Sep.5 [cited 2024Nov.21];68(3):641-9. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/983

Issue

Vol. 68 No. 3 (2016)

Section

Articles

License

Authors grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.