Bioactivity of Juniperus communis essential oil and post-distillation waste: assessment of selective toxicity against food contaminants

Authors

  • Biljana Nikolić Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade http://orcid.org/0000-0003-1765-2454
  • Bojana Vasilijević Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade
  • Ana Ćirić Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade http://orcid.org/0000-0002-9478-5448
  • Dragana Mitić-Ćulafić Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade
  • Stefana Cvetković Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade
  • Ana Džamić Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade
  • Jelena Knežević-Vukčević Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade

Keywords:

Juniperus communis, essential oil, post-distillation waste, selective antimicrobial effect, adhesion-inhibition properties

Abstract

Paper description:

  • Juniperus communis is used as a common spice in food preparations. The essential oil (EO) of J. communis is widely used in alcoholic beverage and cosmetic industries. After distillation of EO, numerous biologically active compounds remain in post-distillation waste (PDW).
  • We monitored the antimicrobial effect of J. communis EO and PDW against food contaminants. The antimicrobial properties of J. communis PDW, including the in vitro potential to reduce L. monocytogenes adhesion to intestinal cells, are reported for the first time.
  • The obtained results provide new insight into the use of J. communis PDW as a potential natural antimicrobial food preservative.

Abstract: Previously chemically characterized Juniperus communis essential oil (EO) and post-distillation waste (PDW) were tested for cytotoxicity and antimicrobial activity against food contaminants. Microdilution assay showed that PDW induced moderate antifungal (minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values, ranging between 0.118-0.900 mg mL-1), and an antibacterial effect against Listeria monocytogenes (MIC and minimum bactericidal concentration (MBC) were 0.39 and 0.74 mg mL-1, respectively). Combinations of EO/PDW with selected antibiotics induced synergistic antilisterial activity in the checkerboard assay. The MTT assay determined that cytotoxicity against colon cancer cells was high for the EO but negligible for PDW (IC50 values were 0.087-0.106 and 1.450-6.840 mg mL-1, respectively). The selectivity indices indicated high selectivity of PDW against tested fungi and L. monocytogenes. In the adhesion-inhibition assay, PDW reduced in vitro adhesion of L. monocytogenes to colon cells (29-62% of inhibition). In conclusion, PDW exhibited an antimicrobial effect against important food spoilage and poisoning fungi and L. monocytogenes, and also reduced in vitro adhesion of L. monocytogenes to colon cells. The results indicate that J. communis PDW could be considered as natural preservative against food spoilage and poisonous fungi, and as an adjuvant to conventional therapy of listeriosis.

https://doi.org/10.2298/ABS181217005N

Received: December 17, 2018; Revised: January 31, 2019; Accepted: February 1, 2019; Published online: February 21, 2019

How to cite this article: Nikolić B, Vasilijević B, Ćirić A, Mitić-Ćulafić D, Cvetković S, Džamić A, Knežević-Vukčević J. Bioactivity of Juniperus communis essential oil and post-distillation waste: Assessment of selective toxicity against food contaminants. Arch Biol Sci. 2019;71(2):235-44.

Downloads

Download data is not yet available.

References

Blair JM, Webber MA, Baylay AJ, Ogbolu DO, Piddock LJ. Molecular mechanisms of antibiotic resistance. Nat Rev Microbiol. 2015;13(1):42-51.

Wiederhold NP. Antifungal resistance: current trends and future strategies to combat. Infect Drug Resist. 2017;10:249-59.

Savoia D. Plant-derived antimicrobial compounds: alternatives to antibiotics. Future microbial. 2012;7(8):979-90.

Adams RP. Junipers of the world: the genus Juniperus. 4th ed. Waco, USA: Trafford Publishing; 2014. 422 p.

Khan M, Khan AU, Gilani AH. Pharmacological explanation for the medicinal use of Juniperus excelsa in hyperactive gastrointestinal and respiratory disorders. J Nat Med. 2012;66(2):292-301.

Leporatti ML, Ivancheva S. Preliminary comparative analysis of medicinal plants used in traditional medicine of Bulgaria and Italy. J Ethnopharmacol. 2003;87(2-3):123-42.

Elmastaş M, Gülçin İ, Beydemir Ş, İrfan Küfrevioğlu Ö, Aboul‐Enein HY. A study on the in vitro antioxidant activity of juniper (Juniperus communis L.) fruit extracts. Anal Lett. 2006;39(1):47-65.

Kurti L, Jovanova B, Kelmendi A, Hamidi M, Kadifkova-Panovska T, Kulevanova S. Antioxidant activity of Macedonian Juniper (Juniperus communis L.) fruit extracts. Toxicol Lett. 2015;238(2):S89.

Orhan N, Aslan M, Demirci B, Ergun F. A bioactivity guided study on the antidiabetic activity of Juniperus oxycedrus subsp. oxycedrus L. leaves. J Ethnopharmacol. 2012;140(2):409-15.

Zheljazkov VD, Semerdjieva IB, Dincheva I, Kacaniova M, Astatkie T, Radoukova T, Schlegel V. Antimicrobial and antioxidant activity of Juniper galbuli essential oil constituents eluted at different times. Ind Crops Prod. 2017;109:529-37.

Golebiowski M, Paszkiewicz M, Halinski L, Malinski E, Stepnowski P. Chemical composition of commercially available essential oils from Eucalyptus, Pine, Ylang, and Juniper. Chem Nat Compd. 2009;45(2):278-9.

Ciesla WM. Non-wood forest products from conifers. 1st ed. Rome: Food and Agriculture Organization of the United Nations; 1998. 124 p.

Lesjak MM, Beara IN, Orčić DZ, Ristić JD, Anačkov GT, Božin BN, Mimica-Dukić NM. Chemical characterisation and biological effects of Juniperus foetidissima Willd. 1806. LWT-Food Sci Technol. 2013;53(2):530-9.

Uríčková V, Sádecká J, Májek P. Classification of Slovak juniper-flavoured spirit drinks. J Food Nutr Res. 2015;54(4):298-307.

Brindza J, Toth D, Ostrovsky R, Kucelova L. Traditional Foods in Slovakia. In: Kristbergsson K, Oliveira J, editors. Traditional Foods. General and Consumer Aspects. New York, USA: Springer; 2016. p. 71-84.

Nybe EV, Mini Raj N, Peter KV. Spices. 1st ed. New Delhi: New India Publishing Agency; 2007. 316 p. (Horticulture science series; vol. 5).

Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010;4(8):118-26.

Wilson BG, Bahna SL. Adverse reactions to food additives. Ann Allergy Asthma Immunol. 2005;95(6):499-507.

Si W, Gong J, Tsao R, Zhou T, Yu H, Poppe C, Johnson R, Du Z. Antimicrobial activity of essential oils and structurally related synthetic food additives towards selected pathogenic and beneficial gut bacteria. J Appl Microbiol. 2006;100(2):296-305.

Gavarić N, Kovač J, Kretschmer N, Kladar N, Možina SS, Bucar F, Bauer R, Božin B. Natural Products as Antibacterial Agents — Antibacterial Potential and Safety of Post-distillation and Waste Material from Thymus vulgaris L., Lamiaceae. In: Bobbarala V, editor. Concepts, Immunology and Microbiology: Compounds and the Alternatives of Antibacterials. Rijeka, Croatia: InTech; 2015. p. 123-52.

Vasilijević B, Knežević-Vukčević J, Mitić-Ćulafić D, Orčić D, Francišković M, Srdic-Rajic T, Jovanović M, Nikolić B. Chemical characterization, antioxidant, genotoxic and in vitro cytotoxic activity assessment of Juniperus communis var. saxatilis. Food Chem Toxicol. 2018;112:118-25.

Sarker SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods, 2007;42:321-4.

Džamić AM, Nikolić BJ, Giweli AA, Mitić‐Ćulafić DS, Soković MD, Ristić MS, Knežević-Vukčević JB, Marin PD. Libyan Thymus capitatus essential oil: antioxidant, antimicrobial, cytotoxic and colon pathogen adhesion‐inhibition properties. J Appl Microbiol. 2015;119(2):389-99.

Mulyaningsih S, Sporer F, Zimmermann S, Reichling J, Wink M. Synergistic properties of the terpenoids aromadendrene and 1, 8-cineole from the essential oil of Eucalyptus globulus against antibiotic-susceptible and antibiotic-resistant pathogens. Phytomedicine. 2010;17(13):1061-6.

Nunes BC, Martins MM, Chang R, Morais SA, Nascimento EA, de Oliveira A, Cunha LCS, da Silva CV, Teixeira TL, Ambrósio MALV, Martins CHG, de Aquino FJT. Antimicrobial activity, cytotoxicity and selectivity index of Banisteriopsis laevifolia (A. Juss.) B. Gates leaves. Ind Crops Prod. 2016;92:277-89.

Cavaleiro C, Pinto E, Gonçalves MJ, Salgueiro L. Antifungal activity of Juniperus essential oils against dermatophyte, Aspergillus and Candida strains. J Appl Microbiol. 2006;100(6):1333-8.

Taviano MF, Marino A, Trovato A, Bellinghieri V, Melchini A, Dugo P, Cacciola F, Donato P, Mondello L, Güvenç A, De Pasquale R, Miceli N. Juniperus oxycedrus L. subsp. oxycedrus and Juniperus oxycedrus L. subsp. macrocarpa (Sibth. & Sm.) Ball.“berries” from Turkey: Comparative evaluation of phenolic profile, antioxidant, cytotoxic and antimicrobial activities. Food Chem Toxicol. 2013;58:22-9.

Glišić SB, Milojević SŽ, Dimitrijević SI, Orlović AM, Skala DU. Antimicrobial activity of the essential oil and different fractions of Juniperus communis L. and a comparison with some commercial antibiotics. J Serb Chem Soc. 2007;72(4):311-20.

Cabral C, Francisco V, Cavaleiro C, Gonçalves MJ, Cruz MT, Sales F, Batista MT, Salgueiro L. Essential oil of Juniperus communis subsp. alpina (Suter) Čelak needles: chemical composition, antifungal activity and cytotoxicity. Phytother Res. 2012;26(9):1352-7.

Fierascu I, Ungureanu C, Avramescu SM, Cimpeanu C, Georgescu MI, Fierascu RC, Ortan A, Sutan AN, Anuta V, Zanfirescu A, Dinu-Pirvu CE, Valescu BS. Genoprotective, antioxidant, antifungal and anti-inflammatory evaluation of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills. BMC Complement Altern Med. 2018;18(1):3.

Holetz FB, Pessini GL, Sanches NR, Cortez DAG, Nakamura CV, Dias Filho BP. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz. 2002;97(7):1027-31.

Adeyeye SA. Fungal mycotoxins in foods: A review. Cogent Food Agric. 2016;2:1213127.

Ashiq S. Natural occurrence of mycotoxins in food and feed: Pakistan perspective. Compr Rev Food Sci Food Saf. 2015;14(2):159-75

Ahmed D, Eide PW, Eilertsen IA, Danielsen SA, Eknaes M, Hektoen M, Lind GE, Lothe RA. Epigenetic and genetic features of 24 colon cancer cell lines. Oncogenesis. 2013;2(9):e71.

Filipowicz N, Kamiński M, Kurlenda J, Asztemborska M, Ochocka JR. Antibacterial and antifungal activity of juniper berry oil and its selected components. Phytother Res. 2003;17(3):227-31.

Griffin SG, Wyllie SG, Markham JL, Leach DN. The role of structure and molecular properties of terpenoids in determining their antimicrobial activity. Flavour Frag J. 1999;14(5):322-32.

Daglia M. Polyphenols as antimicrobial agents. Curr Opin Biotechnol. 2012;23(2):174-81.

Milenković M, Stošović J, Slavkovska V. Synergy between Essential Oils of Calamintha Mill. Species (Lamiaceae) and Antibiotics. Nat Prod Commun. 2018;13(3):371-4.

Cunha BA. Antibiotic side effects. Med Clin North Am. 2001;85(1):149-85.

Lee JH, Shim JS, Chung MS, Lim ST, Kim KH. In vitro anti-adhesive activity of green tea extract against pathogen adhesion. Phytother Res. 2009;23(4):460-6.

Klančnik A, Zorko Š, Toplak N, Kovač M, Bucar F, Jeršek B, Smole Možina S. Antiadhesion activity of juniper (Juniperus communis L.) preparations against Campylobacter jejuni evaluated with PCR-based methods. Phytother Res. 2017; 32(3):542-50.

Downloads

Published

2019-06-04

How to Cite

1.
Nikolić B, Vasilijević B, Ćirić A, Mitić-Ćulafić D, Cvetković S, Džamić A, Knežević-Vukčević J. Bioactivity of Juniperus communis essential oil and post-distillation waste: assessment of selective toxicity against food contaminants. Arch Biol Sci [Internet]. 2019Jun.4 [cited 2024Dec.26];71(2):235-44. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/3760

Issue

Vol. 71 No. 2 (2019)

Section

Articles

License

Authors grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to share the work with an acknowledgment of the work’s authorship and initial publication in this journal.

Most read articles by the same author(s)