Headspace volatiles isolated from twigs of Picea omorika from Serbia

Biljana Nikolić; Jovana Ljujić; Srđan Bojović; Zorica Mitić; Nemanja Rajčević; Vele Tešević; Petar D. Marin

Authors

Biljana Nikolić Department of Genetics, Plant Breeding, Seed and Nursery Production, Institute of Forestry, Kneza Višeslava 3, 11000 Belgrade http://orcid.org/0000-0002-2436-8294
Jovana Ljujić Faculty of Chemistry, University of Belgrade, Studentski trg 16, 11000 Belgrade http://orcid.org/0000-0002-0215-0155
Srđan Bojović Department of Ecology, Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, Department of Ecology, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade http://orcid.org/0000-0002-5795-314X
Zorica Mitić Faculty of Sciences and Mathematics, Department of Biology and Ecology, University of Niš, Višegradska 33, 18000 Niš http://orcid.org/0000-0001-9628-3882
Nemanja Rajčević Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade http://orcid.org/0000-0003-2260-1205
Vele Tešević Faculty of Chemistry, University of Belgrade, Studentski trg 16, 11000 Belgrade http://orcid.org/0000-0001-7060-9055
Petar D. Marin Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade http://orcid.org/0000-0002-9460-1012

Keywords:

Picea omorika, headspace method, PCA, cluster, volatile compounds

Abstract

Paper description:

The headspace method (HS) enables us to study the composition of volatile compounds by using only 0.1 g of plant material and without creating artefacts during isolation.
Volatiles stored in twigs are separated from needle volatiles and thus add new information to chemophenetic studies.
Volatiles of 100 individuals of P. omorika collected in natural populations from seven localities were extracted for the first time using by HS. The composition was analysed statistically to assess variability and population differentiation.
The dominant compounds were α-pinene (29.5 %), β-pinene (25.7%), and myrcene (13.0%), representing 68.2% of the HS volatiles on average.

Abstract: The variability of volatiles isolated from twigs by the static headspace (HS) method in seven natural populations of Picea omorika from Serbia was investigated for the first time. In the overall chemical profile, monoterpenes strongly dominated hydrocarbons as the most volatile compounds (95.7%). The dominant compounds were α-pinene (29.5 %), β-pinene (25.7%) and myrcene (13.0%), totaling 68.2% of the volatiles on average. The following nine volatiles were found to be present in medium-to-high amounts (0.5-10%): tricyclene, camphene, α-phellandrene, δ-3-carene, p-cymene, β-phellandrene, terpinolene, (E)-caryophyllene, and germacrene D. Out of the 78 volatiles detected, the six most abundant (α-pinene, β-pinene, myrcene, δ-3-carene, β-phellandrene and camphene) were selected for principle component analysis (PCA) and cluster analyses (CA). PCA revealed a high degree of similarity between populations, while CA showed a degree of separation of two populations from the others. The presented results are in agreement with previous phytochemical and molecular analyses of this species that confirm high variability in both specialized metabolites and genetic markers.

https://doi.org/10.2298/ABS200511038N

Received: May 11, 2020; Revised: August 19, 2020; Accepted: August 24, 2020; Published online: August 27, 2020

How to cite this article: Nikolić B, Ljujić J, Bojović S, Mitić Z, Rajčević N, Tešević V, Marin PD. Headspace volatiles isolated from twigs of Picea omorika from Serbia. Arch Biol Sci. 2020;72(3):445-52.

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Headspace volatiles isolated from twigs of Picea omorika from Serbia

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