Biology, morphology, and phylogeny of some strains of the Pleurotus eryngii species complex
DOI:
https://doi.org/10.2298/ABS220524026BKeywords:
Pleurotus eryngii var. ferulae, P. tuoliensis, mycelium, pure culture, phylogenetic identificationAbstract
Paper description:
- Preserving valuable edible and medicinal mushrooms is critical. An effective method is to maintain them in culture collections in vitro with correct strain identification and verification.
- Strains from the Pleurotus eryngii species complex were studied using molecular-genetic, cultural-morphological and physiological methods.
- The manuscript contains new data on growth physiology and original scanning electron micrographs of Pleurotus tuolescens and eryngii var. ferula microstructures.
- A critical comparative analysis of DNA sequences entered in the GenBank as nebrodensis was made. We show that physiological and morphological characteristics can serve as additional criteria for the verification of pure mushroom cultures.
Abstract: Effective methods of preserving the gene pool of valuable edible and medicinal mushrooms are to maintain them in in vitro culture collections and to correctly identify and verify the preserved strains. It is necessary to consider not only the results of molecular genetic studies but also cultural, morphological and physiological characteristics as additional criteria. This article presents data on the colony and mycelial morphology, growth characteristics and temperature tolerance, and phylogenetical placement of four strains of edible and medicinal mushroom from the P. eryngii species complex received into the IBK Mushroom Collection as P. nebrodensis strains. All the studied strains are mesophiles with the fastest growth rate of 11.0 mm/day at 26°С and a lethal temperature of 40°С. In addition to common anastomoses, mycelial strands and clamp connections, the vegetative mycelium of the studied strains formed single colorless round excretory cells on the lateral hyphal ramifications. All cultures were able to form primordia and fruit bodies on agar media. Phylogenetic analysis suggests that all four strains do not belong to P. nebrodensis, but two of them, IBK 1947, 2035 are P. eryngii var. ferulae, and two strains, IBK 1855 and 1927, belong to P. tuoliensis (P. eryngii var. tuoliensis).
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Copyright (c) 2022 Nina Bisko , Margarita Lomberg, Oksana Mykchaylova, Nadiya Mytropolska, Vira Kutovenko , Andrii Gryganskyi
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