Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L.

Authors

  • Haote Han The Key Laboratory of Biomedical Engineering, Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027
  • Na Liu Shandong University of Traditional Chinese Medicine, Jinan 250355
  • Lin Zhang The Key Laboratory of Biomedical Engineering, Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027
  • Minghua Gong Shandong University of Traditional Chinese Medicine, Jinan 250355
  • Ming Cao Department of Pharmacology, Wenzhou Medical University, Wenzhou 325035
  • Baoguo Li Shandong University of Traditional Chinese Medicine, Jinan 250355
  • Sulaiman Kaisa The Xinjiang Uygur Autonomous Region National Institute of traditional Chinese Medicine, Urumchi 830092
  • Xiuying Yu The Key Laboratory of Biomedical Engineering, Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027
  • Jingkui Tian The Key Laboratory of Biomedical Engineering, Ministry of Education, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027

Keywords:

daucusol, cytoplasmic vacuolation, oxidative stress, endoplasmic reticulum stress, prostate cancer

Abstract

We investigated the antitumor activity of daucusol (DS) derived from Daucus carota L. in PC-3, A549 and HeLa cell lines by the MTT assay. Optical microscopy revealed that exposure of PC-3 cells to DS resulted in cytoplasmic vacuolation. Flow cytometry analysis of the phase of the cell cycle did not reveal a sub-G1 peak, and no caspase-dependent activation was observed after DS treatment. The levels of endoplasmic reticulum (ER) stress biomarkers, LC3B-II and ubiquitinated proteins were increased. It was also observed that oxidative stress played an important role in the activation of the cytoplasmic vacuolation-mediated cell-death pathway. In vivo, DS inhibited tumor growth in nude mice by 39.13% compared to the vehicle. Protein expression in the tumor tissue was consistent with their expression in vitro. Our findings indicate that DS induced cytoplasmic vacuolation-mediated death in PC-3 cells by triggering oxidative stress and suggest that targeting this pathway could serve as a novel therapeutic approach for prostate cancer.

https://doi.org/10.2298/ABS160902123H

Received: September 2, 2016; Revised: October 1, 2016; Accepted: October 28, 2016; Published online: November 9, 2016

How to cite: Han H, Liu N, Zhang L, Gong M, Cao M, Li B, Kaisa S, Yu X, Tian J. Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L. Arch Biol Sci. 2017;69(3):481-9.

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Published

2017-08-11

How to Cite

1.
Han H, Liu N, Zhang L, Gong M, Cao M, Li B, Kaisa S, Yu X, Tian J. Promotion of cytoplasmic vacuolation-mediated cell death of human prostate cancer PC-3 cells by oxidative stress induced by daucusol, a new guaiane-type sesquiterpenoid from Daucus carota L. Arch Biol Sci [Internet]. 2017Aug.11 [cited 2024Nov.21];69(3):481-9. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/960

Issue

Vol. 69 No. 3 (2017)

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Articles

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