Altered metabolic pathways in classic and hypervirulent Klebsiella pneumoniae isolates revealed by proteomics analysis

Authors

  • Hui Yu 1. School of Basic Medicine, Baotou Medical College, Baotou 014060, China; 2. Inner Mongolia Key Laboratory of Disease-Related Biomarkers, Baotou Medical College, Baotou 014060, China
  • Lixia Zhang The First Affiliated Hospital, Baotou Medical College, Baotou 014010, China
  • Rina Su The Second Affiliated Hospital, Baotou Medical College, Baotou 014030, China
  • Hai Hu School of Basic Medicine, Baotou Medical College, Baotou 014060, China
  • Zhanli Wang Inner Mongolia Key Laboratory of Disease-Related Biomarkers, Baotou Medical College, Baotou 014060, China

DOI:

https://doi.org/10.2298/ABS220613022Y

Keywords:

Klebsiella pneumoniae, Virulence, Proteomics, Metabolic pathway

Abstract

Paper description:

  • Differences between the virulence and pathogenic characteristics of hypervirulent Klebsiella pneumoniae and classical pneumoniae are poorly understood.
  • Using comparative proteomics we explored the correlation between the expression characteristics of proteins and hypervirulence.
  • Of 451 significantly differentially expressed proteins, 185 were significantly upregulated and 266 were significantly downregulated. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that the differentially expressed proteins were predominantly metabolism associated.
  • Our results contribute to a better understanding of the virulence and pathogenic characteristics of hypervirulent pneumoniae.

Abstract: Klebsiella pneumoniae is an opportunistic pathogen that causes a wide range of infections. The emergence and spread of hypervirulent K. pneumoniae (hvKp), which appears to be different from the classical K. pneumoniae (cKp) in several microbiological aspects, is an urgent global threat. However, the virulence characteristics of hvKp and its differences from cKp are poorly understood. This work aimed to investigate the correlation between the expression characteristics of proteins and hypervirulence, using proteomics. Our results revealed that 185 proteins were upregulated while 266 proteins were downregulated in hvKp isolates when compared with cKp isolates. The differentially expressed proteins were functionally categorized according to the Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. These proteins were predominantly metabolism associated, which indicates that changes in the metabolic pathways in hvKp isolates might in part contribute to hypervirulence.

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Published

2022-10-05

How to Cite

1.
Yu H, Zhang L, Su R, Hu H, Wang Z. Altered metabolic pathways in classic and hypervirulent Klebsiella pneumoniae isolates revealed by proteomics analysis. Arch Biol Sci [Internet]. 2022Oct.5 [cited 2024Dec.25];74(3):235-42. Available from: https://serbiosoc.org.rs/arch/index.php/abs/article/view/7846

Issue

Vol. 74 No. 3 (2022)

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Articles

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Copyright (c) 2022 Hui Yu, Lixia Zhang, Rina Su, Hai Hu, Zhanli Wang

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